Purification, partial characterization, and localization of Sak57, an acidic intermediate filament keratin present in rat spermatocytes, spermatids, and sperm

Molecular Reproduction and Development

Volumn 44, Issue 3, 1996, Pages 382-394

  Kierszenbaum, Abraham L ^a   Rivkin, Eugene ^a   Fefer Sadler, Sandra ^a   Mertz, James R ^a   Tres, Laura L ^a  

^a CITY UNIVERSITY OF NEW YORK MEDICAL SCHOOL   (United States)

Author keywords

Chromatofocusing; Confocal microscopy; Outer dense fibers; Phosphorylation

Indexed keywords

KERATIN;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; RAT; SPERM; SPERMATID; SPERMATOCYTE;

AMINO ACID SEQUENCE; ANIMALS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; KERATINS; MALE; MICE; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; PROTEIN PRECURSORS; RABBITS; RATS; RATS, SPRAGUE-DAWLEY; SEQUENCE HOMOLOGY, AMINO ACID; SPERMATIDS; SPERMATOCYTES; SPERMATOZOA; TESTIS;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0029767394     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199607)44:3<382::AID-MRD12>3.0.CO;2-2     Document Type: Article

References (40)

1. Abdullah M, Tres LL, Ueda H, Hu P-C, Kierszenbaum AL (1988): Antigenic homology between rat sperm tail polypeptides and Sertoli cell secretory proteins. Mol Cell Biochem 81:165-176.
2. Burfeind P, Hoyer-Fender S (1991): Sequence and developmental expression of a mRNA encoding a putative protein of rat sperm outer dense fibers. Dev Biol 148:195-204.
3. Calvin HI (1979): Electrophoretic evidence for the identity of the major zinc-binding polypeptides in the rat sperm tail. Biol Reprod 21: 873-882.
4. Calvin HI, Bedford JM (1971): Formation of disulphide bonds in the nucleus and accessory structures of mammalian spermatozoa during epididymal maturation. J Reprod Fert Suppl 13:65-75.
5. Calvin HI, Hwang FH-F, Wohlrab H (1975): Localization of zinc in a dense fiber-connecting piece fraction of rat sperm tails analogous chemically to hair keratin. Biol Reprod 13:228-239.
6. Fernandez J, DeMott M, Atherton D, Mische SM (1992): Internal protein sequence analysis: Enzymatic digestion for less than 10 μg of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal Biochem 201:255-264.
7. Franke WW, Grand C, Schmid E (1979): Intermediate-sized filaments present on Sertoli cells are of the vimentin type. Eur J Cell Biol 19:269-275.
8. Hecht NB, Distel JR, Yelick PC, Tanhauser SM, Driscoll CE, Goldberg E, Tung KSK (1988): Localization of a highly divergent mammalian testicular α-tubulin that is not detectable in brain. Mol Cell Biol 8:996-1000.
9. Irons MJ, Clermont Y (1982a): Kinetics of fibrous sheath formation in the rat spermatid. Am J Anat 165:121-130.
10. Irons MJ, Clermont Y (1982b): Formation of the outer dense fibers during spermiogenesis in the rat. Anat Red 202:463-471.
11. Kierszenbaum AL, Crowell JA, Shabanowitz RB, Smith EP, Spruill WA, Tres LL (1986): A monoclonal antibody recognizes a form of intermediate filament protein in rat Sertoli cells that is not present in seminiferous peritubular cells. Biol Reprod 35:227-238.
12. Kierszenbaum AL, Abdullah M, Ueda H, Tres LL (1988): Antibodies to rat sperm tail polypeptides recognize Sertoli cell secretory proteins. Mol Cell Biochem 85:171-179.
13. Kierszenbaum AL, Fefer-Sadler S, Mertz JR, Rivkin E, Tres LL (1994): Outer dense fibers of the rat sperm tail contain a protein with a heptamer amino acid sequence characteristic of keratin type II. Mol Biol Cell 5:97a.
14. Kierszenbaum AL, Ueda H, Tres LL (1991): Immunogold electron microscopic localization of antigenic sites in the outer dense fiber region of rat sperm tail obtained using antisera to two Sertoli cell secretory proteins. J Electron Microsc 21:261-268.
15. Kierszenbaum AL, Rivkin E, Mertz JR, Fefer-Sadler S, Tres LL (1995): Cytokeratins are present in spermatogenic cells. Acta Anat 152:300.
16. Kleene KC, Smith J, Bozorgzadeh A, Harris M, Hahn L, Karimpour I, Gerstel J (1990): Sequence and developmental expression of the mRNA encoding the seleno-protein of the sperm mitochondrial capsule in the mouse. Dev Biol 137:395-402.
17. Kuhn R, Schafer U, Schäfer M (1988): Cis-acting regions sufficient for spermatocyte-specific transcriptional and spermatid-specific translational control of Drosophila melanogaster gene mst(3)gl-9. EMBO 7:447-454.
18. Kuhn R, Kuhn C, Borsch D, Glatzer KH, Schäfer U, Schäfer M (1991): A cluster of four genes selectively expressed in the male germ line Drosophila melanogaster. Mech Dev 35:143-151.
19. Lewis SA, Cowan NJ (1988): Complex regulation and functional versatility of mammalian α and β tubulin isotypes during the differentiation of testis and muscle cells. J Cell Biol 106:2023-2033.
20. Mertz JR, Banda PW, Kierszenbaum AL (1995): Rat sperm galactosyl receptor: Purification and identification by polyclonal antibodies raised against multiple antigen peptides. Mol Reprod Dev 41: 314-383.
21. Morales CR, Oko R, Clermont Y (1994): Molecular cloning and developmental expression of a mRNA encoding the 27 kDa outer dense fiber protein of rat spermatozoa. Mol Reprod Dev 37:229-240.
22. Neesen J, Bunemann H, Heinlei UAO (1994a): The Drosophila hydei gene Dhmst101(1) encodes a testis specific, repetitive, axoneme-associated protein with differential abundance in Y chromosomal deletion mutant flies. Dev Biol 162:414-425.
23. Neesen J, Padmanabhan S, Nunemann H (1994b): Tandemly arranged repeats of a novel highly charged 16-amino acid motif representing the major component of the sperm-tail specific axoneme-associated protein family Dhmst101 form extended α-helical rods within the extremely elongated spermatozoa of Drosophila hydei. Eur J Biochem 225:1089-1095.
24. O'Brien DA, Bellvé AR (1980): Protein constituents of the mouse spermatozoon. I. An electrophoretic characterization. Dev Biol 75:386-404.
25. Oko R (1988): Comparative analysis of proteins from the fibrous sheath and outer dense fibers of rat spermatozoa. Biol Reprod 39:169-182.
26. Oko R, Clermont Y (1988): Isolation, structure and protein composition of the perforatium of rat spermatozoa. Biol Reprod 39:673-687.
27. Oko R, Clermont Y (1989): Light microscopic immunocytochemical study of fibrous sheath and outer dense fiber formation in the rat spermatid. Anat Rec 225:46-55.
28. Olson GE, Hamilton DW, Fawcett DW (1976): Isolation and characterization of the fibrous sheath of rat epididymal spermatozoa. Biol Reprod 14:517-530.
29. Olson GE, Sammons DW (1980): Structural chemistry of outer dense fibers in rat sperm. Biol Reprod 22:319-322.
30. Schäfer U (1986) Genes for male specific transcripts in Drosophila melanogaster. Mol Gen Genet 202:219-225.
31. Slaughter GR, Meistrich ML, Means AR (1989): Expression of RNAs for calmodulin, actin and tubulins in rat testis cells. Biol Reprod 40:395-405.
32. Smith FF, Mertz JR, Krebs I, Tres LL, Chae C-B, Zakeri Z, Engelhardt J, Hoover D, Tenniswood M, Kierszenbaum AL (1992): Rat Sertoli and spermatogenic cells express a similar gene and its product is antigenically related to an outer dense fiber-associated protein. Mol Reprod Dev 33:363-372.
33. Steinert P, Zackroff R, Aynardi-Whitman M, Goldman RD (1982): Isolation and characterization of intermediate filaments. Methods Cell Biol 24:399-419.
34. Steinert PM, Parry DAD, Idler WW, Johnson LD, Steven AC, Roop DR (1985): Amino acid sequences of mouse and human epidermal type II keratins of Mr 67,000 provide a systematic basis for the structural and functional diversity of the end domains of keratin intermediate filament subunits. J Biol Chem 260:7142-7149.
35. Tam JP (1988): Synthetic peptide vaccine design: Synthesis and properties of a high-density multiple antigen peptide system. Proc Natl Acad Sci U S A 85:5409-5413.
36. Tres LL, Mertz JR, Fefer-Sadler S, Rivkin E, Kierszenbaum AL (1994): Rat spermatogenic cells contain a cytokeratin with a heptamer amino acid motif identical to keratin type II. Mol Biol Cell 5:296a.
37. Tres LL, Kierszenbaum AL (1996): Sak 57, an acidic keratin initially present in the spermatid manchette before becoming a component of paraaxonemal structures of the developing tail. Mol Reprod Dev (in press).
38. Van Der Horn FA, Tarnasky HE (1992): Factors involved in regulation of the RT7 promoter in a male germ cell-derived in vitro transcription system. Proc Natl Acad Sci U S A 89:703-707.
39. Vera JC, Brito M, Zuvic T, Burzio LO (1984): Polypeptide composition of rat sperm outer dense fibers. J Biol Chem 259:5970-5977.
40. Villasante AD, Wang D, Dobner P, Lewis SA, Cowan NJ (1986): Six mouse-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes. Mol Cell Biol 6:2409-2419.