Chemiluminescent detection of apoptotic DNA: A qualitative and quantitative method

BioTechniques

Volumn 21, Issue 2, 1996, Pages 214-216

  Phelouzat, Marie Anne ^a,b   Laforge, Térèse ^a   Quadri, Rafael A ^a   Arbogast, Agnès ^a   Proust, Jacques J ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b GENEVA UNIVERSITY HOSPITALS   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

APOPTOSIS; ARTICLE; CHEMOLUMINESCENCE; DNA CONTENT; DNA DETERMINATION; FRACTIONATION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; QUALITATIVE DIAGNOSIS; QUANTITATIVE DIAGNOSIS;

EID: 0029765539     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212bm09     Document Type: Article

References (7)

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2. Facchinetti, A., L. Tessarollo, M. Mazzochi, R. Kingston, D. Collavo and G. Biasi. 1991. An improved method for the detection of DNA fragmentation. J. Immunol. Methods 136:125-131.
3. Gavrieli, Y., Y. Sherman and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear fragmentation. J. Cell Biol. 119:493-501.
4. Gold, R., M. Schmied, G. Giegerich, H. Breitschopf, H.P. Hartung, K.V. Toyka and H. Lassmann. 1994. Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques. Lab. Invest. 71:219-225.
5. Gold, R., M. Schmied, G. Rothe, H. Zichler, H. Breitschopf, H. Wekerle and H. Lassmann. 1993. Detection of DNA fragmentation in apotosis: application of in situ nick translation to cell culture systems and tissue sections. J. Histochem. Cytochem. 41:1023-1030.
6. Goldman, D. and C.R. Merril. 1982. Silver staining of DNA in polyacrylamide gels: linearity and effect of fragment size. Electrophoresis 3:24-26.
7. Tilly, J.L. and A.J.W. Hsueh. 1993. Microscale autoradiographic method for the qualitative and quantitative analysis of apoptotic DNA fragmentation. J. Cell. Physiol. 154:519-526.