Time resolution of binding and membrane insertion of a mitochondrial signal peptide: Correlation with structural changes and evidence for cooperativity

Biochemistry

Volumn 35, Issue 33, 1996, Pages 10931-10937

  Golding, Caroline ^a   Senior, Simon ^b   Wilson, Michael T ^a   O'Shea, Paul ^a  

^a UNIVERSITY OF ESSEX   (United Kingdom)

^b Applied Photophysics Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME C OXIDASE;

ARTICLE; CIRCULAR DICHROISM; DISSOCIATION CONSTANT; FLUORESCENCE; MEMBRANE BINDING; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SPECTROMETRY; TEMPERATURE SENSITIVITY;

CELL MEMBRANE; CIRCULAR DICHROISM; ELECTROCHEMISTRY; ELECTRON TRANSPORT COMPLEX IV; ENZYME PRECURSORS; KINETICS; MITOCHONDRIA; PHOSPHOLIPIDS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN SORTING SIGNALS; SPECTROMETRY, FLUORESCENCE;

EID: 0029764221     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi960905i     Document Type: Article

References (18)

1. Blobel, G., & Dobberstein, D. (1975) Transfer of proteins across membranes, J. Cell Biol. 67, 835-862.
2. Chupin, V., Leenhouts, J. M., de Kroon, A., & de Kruijff, B. (1995) Cardiolipin modulates the secondary structure of the presequence peptide of cytochrome oxidase subunit IV, FEBS Lett. 373, 239-244.
3. DeGrado, W. F. (1993) Catalytic molten globules, Nature 365, 488-489.
4. Gilmore, R., & Blobel, G. (1982) Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants, Cell 42, 497-505.
5. Golding, C., & O'Shea, P. (1995) The interactions of signal sequences with membranes, Biochem. Soc. Symp. 23, 971-976.
6. Keibler, M., Becker, K., Pfanner, N., & Neupert, W. (1993) Mitochondrial protein import - specific recognition and membrane translocation of preproteins, J. Membr. Biol. 135, 191-207.
7. Leenhouts, J. M., Torok, Z., Chupin, V., & de Kniijff, B. (1995) A molecular model for the specific cardiolipin-presequence interactions, Biochem. Soc. Symp. 23, 968-971.
8. Maduke, M., & Roise, D. (1993) The import of a mitochondrial presequence into protein-free phospholipid vesicles, Science 260, 364-367.
9. Martoglio, M., Hofmann, M. W., Brunner, J., & Dobberstein, B. (1995) The protein-conducting channel in the membrane of the endoplasmic reticulum is open laterally towards the bilayer, Cell 81, 207-214.
10. Mayer, A., Nargang, F. E., Neupert, W., & Lill, R. (1995) MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19, EMBO J. 14, 4204-4211.
11. McLaughlin, S. (1989) The electrostatic properties of membranes, Ann. Rev. Biophys. Biophys. Chem. 18, 113-136.
12. Stuart, R. A., Cyr, D. M., & Neupert, W. (1994) HSP70 in mitochondrial biogenesis, Experientia 50, 1002-1011.
13. Tamm, L. (1991) Membrane insertion and lateral mobility of synthetic amphiphilic signal peptides in lipid model membranes, Biochim. Biophys. Acta 1071, 123-148.
14. von Heijne, G. (1986) Mitochondrial targeting sequences may form amphiphilic helices, EMBO. J. 5, 1335-1342.
15. Wall, J., Golding, C., van Veen, M., & O'Shea, P. (1995a) The use of fluoresceinphosphatidylethanolamine as a real-time probe of peptide-membrane interactions. Mol. Membr. Biol. 12, 183-192.
16. Wall, J., Ayoub, F., & O'Shea, P. (1995b) The interactions of macromolecules with the mammalian cell surface, J. Cell Sci. 108, 2673-2682.
17. Watts, A. (1995) Biophysics of the membrane interface, Biochem. Soc. Symp. 23, 959-965.
18. White, S. H., & Wimley, W. C. (1994) Peptides in lipid bilayers-structural and thermodynamic basis for partitioning and folding, Curr. Opin. Struct. Biol. 4, 79-86.