High-throughput asymmetric-PCR SSCP analysis using well-controlled temperature conditions

BioTechniques

Volumn 21, Issue 4, 1996, Pages 710-716

  Kiyama, Masaharu ^a   Fujita, Takeshi ^a  

^a HITACHI LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ELECTRIC FIELD; GENE MUTATION; GENETIC POLYMORPHISM; HEAT TRANSFER; MATHEMATICAL COMPUTING; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SINGLE STRAND CONFORMATION POLYMORPHISM; TEMPERATURE SENSITIVE MUTANT;

EID: 0029764084     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214rr04     Document Type: Article

References (20)

1. Ainsworth, P.J., L.C. Surh and M.B. Coulter-Mackie. 1991. Diagnostic single strand conformational polymorphism (SSCP): a simplified non-radioisotopic method as applied to a Tay-Sachs B1 variant. Nucleic Acids Res. 19:405-406.
2. Carrington, M., T. Miller, M. White, B. Gerrard, C. Stewart, M. Dean and D. Mann. 1992. Typing of HLA-DQA1 and DQB1 using DNA single-strand conformation polymorphism. Hum. Immunol. 53:208-212.
3. Fujita, T. and M. Kiyama. 1995. Identification of DNA polymorphism by asymmetric-PCR SSCP. BioTechniques 19:532-534.
4. Gyllensten, U.B. and H.A. Erlich. 1988. Generation of single-strand DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc. Natl. Acad. Sci. USA 85:7652-7656.
5. Heiger, D.N., A.S. Cohen and B.L. Karger. 1990. Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields. J. Chromatogr. 516:33-48.
6. Holeman, J.P. Heat Transfer. Metric Editions. McGraw-Hill, New York. p. 27-42.
7. Hongyo, T., G.S. Buzard, R.J. Calvert and C.M. Weghost. 1993. 'Cold SSCP': a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses. Nucleic Acids Res. 21:3637-3642.
8. Iwahana, H., K. Yoshimoto, N. Mizusawa, E. Kudo and M. Itakura. 1994. Multiple fluorescence-based PCR-SSCP analysis. BioTechniques 16:296-305.
9. Kambara, H., T. Nishikawa, Y. Katayama and T. Yamaguchi. 1988. Optimization of parameters in a DNA sequencer using fluorescence detection. Bio/Technology 6:816-821.
10. Kuypers, A.W.H.M., P.M.W. Willems, P.C.M. Lissen, H.M.C. Wessels, C.H.M.M. de Bruijin, F.M. Everaerts and E.B.M. Mensink. 1993. Detection of point mutation in DNA using capillary electrophoresis in a polymer network. J. Chromatogr. 621:149-156.
11. Lee, H.H. 1994. An inexpensive cooling fan for SSCP gel electrophoresis. BioTechniques 16:790.
12. Luckey, J.A. and L.M. Smith. 1993. A model for the mobility of single-stranded DNA in capillary gel electrophoresis. Electrophoresis 14:492-501.
13. Mayer, P., G. Slater and G. Drouin. 1993. Exact behavior of single-stranded DNA electrophoretic mobilities in polyacrylamide gels. Appl. Theor. Electrophor. 3:147-155.
14. Mohabeer, A.J., A.L. Hiti and W.J. Martin. 1991. Non-radioactive single strand conformation polymorphism (SSCP) using the Pharmacia 'PhastSystem'. Nucleic Acids Res. 19:3154.
15. Mouradian, S., R.L. Brumley, Jr. and L.M. Smith. 1994. Separating field strength, temperature, and pulsing effects in pulsed field electrophoresis. Electrophoresis 15:1084-1090.
16. Nishikawa, T. and H. Kambara. 1991 Analysis of limiting factors of DNA band separation by a DNA sequencer using fluorescence detection. Electrophoresis 12:623-631.
17. Orita, M., Y. Suzuki, T. Sekiya and K. Hayashi. 1989. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874-877.
18. Oto, M., S. Miyake and Y. Yuasa. 1993. Optimization of nonradioisotopic single strand conformation polymorphism analysis with a conventional minislab gel electrophoresis apparatus. Anal. Biochem. 213:19-22.
19. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Vol. 2, p. 9.17-9.19.
20. Slater, G.W., J. Rousseau, J. Noolandi, C. Turmel and M. Lalande. 1988. Quantitative analysis of the three regimes of DNA electrophoresis in agarose gels. Biopolymers 27:509-524.