Simultaneous selection and cloning of transfected eukaryotic cells

BioTechniques

Volumn 21, Issue 4, 1996, Pages 622-624

  Rubio, Nuria ^a   Blanco, Jeronimo ^a,b  

^a Institut de Recerca Oncologica   (Spain)

^b CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL SELECTION; CELL STRAIN 3T3; CONTROLLED STUDY; DNA TRANSFECTION; MOLECULAR CLONING; MOUSE; NONHUMAN;

ANIMALIA; EUKARYOTA;

EID: 0029762959     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214bm11     Document Type: Article

References (5)

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.A. Smith, J.G. Siedman and K. Struhl. 1995. Stable transfer of genes into mammalian cells, p. 9.5.1-9.5.6. Current Protocols in Molecular Biology, Vol. 1. Greene Publishing Associates and John Wiley and Sons, New York.
2. Felgner, P.L., T.R. Gadek, M. Holm, R. Roman, H.W. Chan, M. Wenz, J.P. Northrop, G.M. Ringold and M. Danielsen. 1987. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA 84:7413-7417.
3. Graham, F.L. and A.J. van der Eb. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456-467.
4. Promega Corporation. Luciferase Assay System, Technical Bulletin, No. TB101. 1993. Madison, WI.
5. Wigler, M., A. Pellicer, S. Silvertein and R. Axel. 1978. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell 14:725.