Semi-multiplex PCR technique for screening of abundant transcripts during systematic sequencing of cDNA libraries

BioTechniques

Volumn 21, Issue 4, 1996, Pages 644-649

  Pacchioni, B ^a   Trevisan, S ^a   Gomirato, S ^a   Toppo, S ^a   Valle, G ^a   Lanfranchi, G ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

ANALYTIC METHOD; ARTICLE; DIAGNOSTIC APPROACH ROUTE; DNA LIBRARY; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; GENETIC TRANSFORMATION; LABORATORY; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

EID: 0029762491     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214st01     Document Type: Article

References (21)

1. Adams, M.D., M. Dubnick, A.R. Kerlavage, R. Moreno, J.M. Kelley, T.R. Utterback, J.W. Nagle and J.C. Venter. 1992. Sequence identification of 2,375 human brain genes. Nature 355:632-634.
2. Adams, M.D., J.M. Kelley, J.D. Gocayne, M. Dubnick, M.H. Polymeropoulos, H. Xiao, C.R. Merril, A. Wu, B. Olde, R. Moreno, A.R. Kerlavage, W.R. McCombie and J.C. Venter. 1991. Complementary DNA sequencing: expressed sequence tags and human genome project. Science 252:1651-1656.
3. Adams, M.D., A.R. Kerlavage, C. Fields and J.C. Venter. 1993. 3,400 new expressed sequence tags identify diversity of transcripts in human brain. Nature Genet. 4:256-267.
4. Altschul, S.F., W. Gish, W. Miller, E.W. Myers and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
5. Gieser, L. and A. Swaroop. 1992. Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library. Genomics 13:873-876.
6. Höfte, H., T. Desprez, J. Amselem, H. Chiapello, M. Caboche, A. Moisa, M-F. Jourjon, L. Charpenteau, et al. 1993. An inventory of 1,152 expressed sequence tags obtained by partial sequencing of cDNA from Arabidopsis thaliana. Plant J. 4:1051-1061.
7. Ko, M.S.H. 1990. An "equalized cDNA library" by the reassociation of short double-stranded cDNAs. Nucleic Acids Res. 18:5705-5711.
8. Kong, H.M., R.B. Kucera and W.E. Jack. 1993. Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. J. Biol. Chem. 265:1965-1975.
9. Lanfranchi, G., T. Muraro, F. Caldara, B. Pacchioni, A. Pallavicini, D. Pandolfo, S. Toppo, S. Trevisan, S. Scarso and G. Valle. 1996. Identification of 4,370 expressed sequence tags (ESTs) from a 3′-end specific cDNA library of human skeletal muscle by DNA sequencing and filter hybridization. Genome Res. 6:35-42.
10. Liew, C.C., D.M. Hwang, Y.W. Fung, C. Laurenssen, E. Cukerman, S. Tsui and C.Y. Lee. 1994. A catalogue of genes in the cardiovascular system as identified by expressed sequence tags. Proc. Natl. Acad. Sci. USA 91:10645-10649.
11. Okubo, K., N. Hori, R. Matoba, T. Niiyama, A. Fukushima, Y. Kojima and K. Matsubara. 1992. Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nature Genet. 2:173-179.
12. Patanjali, S.R., S. Parimoo and S.M. Weissman. 1991. Construction of a uniform-abundance (normalized) cDNA library. Proc. Natl. Acad. Sci. USA 88:1943-1947.
13. Pearson, W.R. and D.J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444-2448.
14. Sargent, T.D. 1987. Isolation of differentially expressed genes. Methods Enzymol. 152:423-431.
15. Sargent, T.D. and I.B. Dawid. 1983. Differential gene expression in the gastrula of Xenopus laevis. Science 222:135-139.
16. Sikela, J.M. and C. Auffrey. 1993. Finding new genes faster than ever. Nature Genet. 3:189-191.
17. Takeda, J., H. Yano, S. Eng, Y. Zeng and G.I. Bell. 1993. A molecular inventory of human pancreatic islets: sequence analysis of 1,000 cDNA clones. Hum. Mol. Genet. 2:1793-1798.
18. Uchimiya, H., S. Kidou, T. Shimazaki, S. Aotsuka, S. Takamatsu, R. Nishi, H. Hashimoto, Y. Matsubayashi, N. Kidou, M. Umeda and A. Kato. 1992. Random sequencing of cDNA libraries reveals a variety of expressed genes in cultured cells of rice (Oryza sativa L.). Plant J. 2:1005-1009.
19. Velculescu, V.E., L. Zhang, B. Vogelstein and K.W. Kinzler. 1995. Serial analysis of gene expression. Science 270:484-487.
20. Wan Kim, C., P. Markiewicz, J.J. Lee, C.F. Schierle and J.H. Miller. 1993. Studies of hyperthermophile Termotoga maritima by random sequencing of cDNA and genomic libraries. J. Mol. Biol. 237:960-981.
21. Waterson, R., C. Martin, M. Craxton, C. Huynh, A. Coulson, L. Hillier, R.K. Durban, P. Green, et al. 1992. A survey of expressed genes in Caenorhabditis elegans. Nature Genet. 1:960-981.