Immunogold labeling of yeast cells: An efficient tool for the study of protein targeting and morphological alterations due to overexpression and inactivation of genes

Histochemistry and Cell Biology

Volumn 106, Issue 1, 1996, Pages 115-130

  Binder, M ^c   Hartig, A ^a   Sata, T ^b  

^a LUDWIG BOLTZMANN INSTITUTE FOR LUNG VASCULAR RESEARCH   (Austria)

^b NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

CELL STRUCTURE; GENE REPRESSION; IMMUNOGOLD STAINING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN TARGETING; REVIEW; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0029762310     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02473206     Document Type: Review

References (58)

1. Baba M, Osumi M (1987) Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substituted Klöckera and Saccharomyces cerevisiae yeast cells. J Electron Microsc Technique 5:249-261
2. Bemis LT, Denis CL (1988) Identification of functional regions in the yeast transcriptional activator ADR1. Mol Cell Biol 8:2125-2131
3. Binder M, Schanz M, Hartig A (1991) Vector-mediated overexpression of catalase A in the yeast Saccharomyces cerevisiae induces inclusion body formation. Eur J Cell Biol 54:305-312
4. Boeke JD, Xu H, Fink GR (1988) A general method for the chromosomal amplification of genes in yeast. Science 239:280-282
5. Bosch H van den, Schutgens H, Wanders RJA, Tager JM (1992) Biochemistry of peroxisomes. Annu Rev Biochem 61:157-197
6. Briza P, Breitenbach M, Ellinger A, Segall J (1990) Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae. Genes Dev 4:1775-1789
7. Brown D, Lydon J, McLaughlin M, Stuart-Tilley A, Tyszkowski R, Alper S (1996) Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS). Histochem Cell Biol 105:261-267
8. Byers R, Goetsch L (1975) Behavior of spindle and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae. J Bacteriol 124:511-523
9. Carlemalm E, Villiger W (1989) Low temperature embedding. In: Bullock GR, Petrusz P (eds) Techniques in immunocytochemistry. Academic Press, London, pp 29-45
10. Carlemalm E, Garavito RM, Villiger W (1982) Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc (Oxford) 126:123-143
11. Carlemalm E, Villiger W, Hobot JA, Acetarin JD (1985) Low temperature embedding with Lowicryl resins: two new formulations and some applications. J Microsc (Oxford) 140:55-63
12. Carlson M (1987) Regulation of sugar utilization in Saccharomyces species. J Bacteriol 169:4873-4877
13. Carrell RW, Lehmann H, Hutchison HE (1966) Haemoglobin Koln (beta-98 valine-methionine): an unstable protein causing inclusion body anaemia. Nature 210:915-916
14. Cherry JR, Denis CL (1989) Overexpression of the yeast transcriptional activator ADR1 induces mutation of the mitochondrial genome. Curr Genet 15:311-317
15. Chin DJ, Luskey KL, Anderson RGW, Faust JR, Goldstein JL, Brown MS (1982) Appearance of crystalloid endoplasmic reticulum in compactin-resistant Chinese hamster cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Proc Natl Acad Sci USA 79:1185-1189
16. Danpure CJ, Fryer P, Jennings PR, Allsop J, Griffith S, Cunningham A (1994) Evolution of alanine: glyoxylate aminotransferase 1 peroxisomal and mitochondrial targeting. A survey of its subcellular distribution in the livers of various representatives of the classes Mammalia, Aves and Amphibia. Eur J Cell Biol 64:295-313
17. deDuve C, Baudhuin P (1966) Peroxisomes (microbodies and related particles). Physiol Rev 46:323-357
18. Denis CL, Ciriacy M, Young ET (1981) A positive regulator is required for the accumulation of the functional mRNA for the glucose repressible alcohol dehydrogenase from Saccharomyces cerevisiae. J Mol Biol 148:355-368
19. Distel B, Veenhuis M, Tabak HF (1987) Import of alcohol oxidase into peroxisomes of Saccharomyces cerevisiae. EMBO J 6:3111-3116
20. Einerhand AWC, Kos WT, Distel B, Tabak HF (1993) Characterization of a transcriptional control element involved in proliferation of peroxisomes in yeast in response to oleate. Eur J Biochem 214:323-331
21. Entian KD (1986) Glucose repression: a complex regulatory system in yeast. Microbiol Sci 3:366-371
22. Erdmann R, Veenhuis M, Mertens D, Kunau WH (1989) Isolation of peroxisome deficient mutants of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 86:5419-5423
23. Filipits M, Simon MM, Rapatz W, Hamilton B, Ruis H (1993) A Saccharomyces cerevisiae upstream activating sequence mediates induction of peroxisome proliferation by fatty acids. Gene 132:49-55
24. Gancedo JM, Gancedo C (1986) Catabolite repression mutants of yeast. FEMS Microbiol Rev 32:179-187
25. Glenner GG (1981) Amyloid deposits and amyloidosis. The beta fibriloses (first of two parts). N Engl J Med 302:1283-1292
26. Görlich D, Vogel F, Mills AD, Hartmann E, Laskey RA (1995) Distinct functions for the two importin subunits in nuclear protein transport. Nature 377:246-248
27. Gould SJ, Keller GA, Subramani S (1987) Identification of a targeting signal at the carboxy terminus of firefly luciferase. J Cell Biol 105:2923-2931
28. Hartig A, Ogris M, Cohen G, Binder M (1990) Fate of highly expressed proteins destined to peroxisomes in Saccharomyces cerevisiae. Curr Genet 18:23-27
29. Hill JE, Myers AM, Koerner TJ, Tzagaloff A (1986) Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2:163-167
30. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163-168
31. Kellenberger E, Dürrenberger M, Villiger W, Carlemalm E, Wurtz M (1987) The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J Histochem Cytochem 35:959-969
32. Kiebler M, Keil P, Schneider H, Klei IJ van der, Pfanner N (1993) The mitochondrial receptor complex: a central role of MOM22 in mediating preprotein transfer from receptors to the general insertion pore. Cell 74:483-492
33. Kirschner DA, Inouye LK, Duffy A, Sinclair M, Lind M, Selkoe DJ (1987) Synthetic peptide homologous to β-protein from Alzheimer disease forms amyloid like fibrils in vitro. Proc Natl Acad Sci USA 84:6953-6957
34. Kolodziej P, Young R (1991) Epitope tagging and protein surveillance. In: Guthrie C, Fink GFR (eds) Methods in enzymology, vol 194: Academic Press, London, pp 508-519
35. Kragler F, Langeder A, Raupachova J, Binder M, Hartig A (1993) Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae. J Cell Biol 120:665-673
36. Krech R, Zerban H, Bannasch P (1981) Mitochondrial anomalies in renal oncocytes induced in rat by N-nitrosomorpholine. Eur J Cell Biol 25:331-339
37. Lazarow PB, Theiringer R, Cohen G, Imanaka T, Small G (1991) Protein import into peroxisomes in vitro. In: Tartakoff AM (ed) Methods in cell biology, vol 34. Academic Press, San Diego, pp 303-326
38. Mitraki A, King J (1989) Protein folding intermediates and inclusion body formation. Biotechnology 7:690-697
39. Robinow CF, Marak J (1966) A fiber apparatus in the nucleus of the yeast cell. J Cell Biol 29:129-151
40. Roggenkamp R, Didion T, Kowallik K von (1989) Formation of irregular giant peroxisomes by overproduction of the crystalloid core protein methanol oxidase in the methylotrophic yeast Hansenula polymorpha. Mol Cell Biol 9:988-994
41. Roth J (1983) The colloidal gold marker system for light and electron microscopy. Theory and application. In: Bullock GR, Petrusz P (ed) Techniques in immunocytochemistry. Academic Press, London, pp 217-284
42. Roth J (1989) Postembedding labeling on Lowicryl K4M tissue sections: detection and modification of cellular components. In: Tartakoff AM (ed) Methods in cell biology, vol 31. Academic Press, San Diego, pp 513-551
43. Rottensteiner H, Kal AJ, Filipits M, Binder M, Hamilton B, Tabak HF, Ruis H (1996) Pip2p: a transcriptional regulator of peroxisome proliferation in the yeast Saccharomyces cerevisiae. EMBO J (in press)
44. Schneider BL, Seufert W, Steiner B, Yang QH, Futcher AB (1995) Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae. Yeast 11:1265-1274
45. Schneider RG, Ueda S, Alperin JB, Brimhall R, Jones T (1989) Hemoglobin Sabine beta 91 (F 7) Leu-Pro. An unstable variant causing severe anaemia with inclusion bodies. N Engl J Med 280:739-745
46. Sherman DM, Troyan TA, Sherman LA (1994) Localization of membrane proteins in the cyanobacterium Synechococcus sp. PCC7942. Radial asymmetry in the photosynthetic complexes. Plant Physiol 106:251-262
47. Shuster J, Yu J, Cox D, Chan RV, Smith M, Young E (1986) ADR1-mediated regulation of ADH2 requires an inverted repeat sequence. Mol Cell Biol 6:1894-1902
48. Simon M, Adam G, Rapatz W, Spevak W, Ruis H (1991) The Saccharomyces cerevisiae ADR1 gene is a positive regulator of transcription of genes encoding peroxisomal proteins. Mol Cell Biol 11:699-704
49. Simon M, Binder M, Adam G, Hartig A, Ruis H (1992) Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1, and SNF4. Yeast 8:303-309
50. Simon MM, Pavlik P, Hartig A, Binder M, Ruis H, Cook WJ, Denis CL, Schanz B (1995) A C-terminal region of Saccharomyces cerevisiae ADR1 transcription factor is important in the regulation of peroxisome proliferation by fatty acids. Mol Gen Genet 249:289-296
51. Takeshige K, Baba M, Tsuboi S, Noda T, Ohsumi Y (1992) Autophagy in yeast demonstrated with protease-deficient mutants and conditions for its induction. J Cell Biol 119:301-311
52. Thukral SK, Tavianni MA, Blumberg H, Young ET (1989) Localization of a minimal binding domain and activation regions in the yeast regulatory protein ADR1. Mol Cell Biol 9:2360-2369
53. Van Tuinen E, Riezman H (1987) Immunolocalization of glyceraldehyde-3-phosphate dehydrogenase, hexokinase and carboxypeptidase Y in yeast cells at the ultrastructural level. J Histochem Cytochem 35:327-333
54. Veenhuis M, Dijken JP van, Harder W (1976) Cytochemical studies on the localization of methanol oxidase and other oxidases in peroxisomes of methanol-grown Hansenula polymorpha. Arch Microbiol 111:123-135
55. Veenhuis M Dijken JP van, Pilon SAF, Harder W (1978) Development of crystalline peroxisomes in methanol-grown cells of the yeast Hansenula polymorpha and its relation to environmental conditions. Arch Microbiol 117:153-163
56. 5 in yeast results in marked proliferation of the intracellular membrane. J Cell Sci 106:249-259
57. Waterham HR, Titorenko VI, Haima P, Cregg JM, Harder W, Veenhuis M (1994) The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals. J Cell Biol 127:737-749
58. Wright R, Basson M, D'Ari L, Rine J (1988) Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus. J Cell Biol 107:101-114