Application of an optimized electroporation procedure for replacement of the polyhydroxyalkanoate synthase I gene in Nocardia corallina

Canadian Journal of Microbiology

Volumn 42, Issue 7, 1996, Pages 715-719

  Valentin, Henry E ^a   Dennis, Douglas ^a  

^a JAMES MADISON UNIVERSITY   (United States)

Author keywords

gene replacement; Nocardia; polyhydroxyalkanoates (PHAs)

Indexed keywords

SYNTHETASE;

ARTICLE; BACTERIAL METABOLISM; ELECTROPORATION; GENE EXPRESSION; NOCARDIA; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

BACTERIA (MICROORGANISMS); CORALLINA; GORDONIA RUBRIPERTINCTUS; NOCARDIA;

EID: 0029761491     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/m96-098     Document Type: Article

References (20)

1. Bonachini, M., and Walden, J.M. 1990. Nocardia brasiliensis peritonitis in a patient with AIDS. Am. J. Gastroenterol. 85: 1432-1433.
2. Coque, J.J.R., Liras, P., Laiz L., and Martín, J.F. 1991. A gene encoding lysine 6-aminotransferase, which forms the β-lactam precursor α-aminoadipic acid, is located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans. J. Bacteriol. 173: 6258-6264.
3. Desomer, J., Dhaese, P., and Van Montagu, M. 1990. Transformation of Rhodococcus fascians by high-voltage electroporation and development of R. fascians cloning vectors. Appl. Environ. Microbiol. 56: 2818-2825.
4. 4-dehydrogenase from Nocardia corallina: purification and characterization. J. Biochem. (Tokyo), 109: 581-586.
5. Ishihashi, Y., Watanabe, R., Hommura, S., Koyama, A., Ishikawa, T., and Mikami, Y. 1990. Endogenous Nocardia asteroides endophtalmitis. Br. J. Ophthalmol. 74: 433-436.
6. Kawakawi, K. 1990. Characterization of production of 1,2-epoxyoctane from 1-octene by nongrowing cells of Nocardia corallina B-176 in aqueous-organic media. Enzyme Eng. 10: 707-711.
7. Madon, J., and Hütter, R. 1991. Transformation system for Amycolatopsis (Nocardia) mediterranei: direct transformation of mycelium with plasmid DNA. J. Bacteriol. 173: 6325-6331.
8. Maniatis, T., Fritsch, E.F., and Sambrook, J. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
9. McNeil, M.M., Brown, J.M., Magruder, C.H., Shearlock, K.T., Saul, R.A., Allred, D.P., and Ajello, L. 1992. Disseminated Nocardia transvalensis infection: an unusual opportunistic pathogen in severely immunocompromised patients. J. Infect. Dis. 165: 175-178.
10. Miller, J.F. 1988. Bacterial electroporation. Mol. Biol. Rep. 5: 1-4.
11. 21-ketosteroids from Rhodococcus rhodochrous: the isolation and characterization. Biochim. Biophys. Acta, 1251: 115-124.
12. Schlegel, H.G., Kaltwasser, H., and Gottschalk, G. 1961. Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 38: 209-222.
13. Schupp, T., and Divers, M. 1986. Protoplast preparation and regeneration in Nocardia mediterranei. FEMS Microbiol. Lett. 36: 159-162.
14. Seto, M., Kimbara, K., Shimura, M., Hatta, T., Fukuda, M., and Yano, K. 1995. A novel transformation of polychlorinated biphenyls by Rhodococcus sp. strain RHA1, Appl. Environ, Microbiol. 61: 3353-3358.
15. Takai S., Imai, Y., Fukunaga, N., Uchida, Y., Kamisawa, K., Sasaki, Y., Tsubaki, S., and Sekizaki, T. 1995. Identification of virulence-associated antigens and plasmids in Rhodococcus equi from patients with AIDS. J. Infect. Dis. 172: 1306-1311.
16. Tarnok, I. 1976. Metabolism in nocardiae and related bacteria. In The biology of the nocardiae. Edited by M. Goodfellow, G.H. Brownell, and J.A. Serrano. Academic Press, New York. pp 451-500.
17. Valentin, H.E., and Dennis, D. 1996. Metabolic pathway for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) formation in Nocardia corallina: inactivation of mutB by chromosomal integration of a kanamycin-resistance gene. Appl. Environ. Microbiol. 62: 372-379.
18. Weijers, C.A.G.M., and deBont, J.A.M. 1991, Enantioselective degradation of 1,2-epoxyalkanes by Nocardia H8. Enzyme Microb. Technol. 13: 306-308.
19. Williamson, J.M., Hock, D., and Inamine, E.S. 1991. HMG-CoA reductase inhibitors produced by Nocardia sp. (MA6455). U.S. Pat. 4 997 755.
20. Yao, W., Yang, Y., and Chiao, J. 1994. Cloning vector system for Nocardia spp. Curr. Microbiol. 29: 223-227.