Characterization of methicillin-resistant Staphylococcus aureus isolates from Portuguese hospitals by multiple genotyping methods

Microbial Drug Resistance

Volumn 2, Issue 3, 1996, Pages 331-341

  De Sousa, Marta Aires ^a   Sanches, Ilda Santos ^a   Van Belkum, Alex ^b   Van Leeuwen, Willem ^b   Verbrugh, Henri ^b   De Lencastre, Hermínia ^a,c  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b ERASMUS MEDICAL CENTER   (Netherlands)

^c ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

METICILLIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM ISOLATION; DNA FINGERPRINTING; GENE AMPLIFICATION; GENOTYPE; GEOGRAPHIC DISTRIBUTION; HOSPITAL INFECTION; NONHUMAN; OLIGONUCLEOTIDE PROBE; PORTUGAL; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; STAPHYLOCOCCUS AUREUS; TANDEM REPEAT;

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 0029758506     PISSN: 10766294     EISSN: None     Source Type: Journal    
DOI: 10.1089/mdr.1996.2.331     Document Type: Article

References (13)

1. Boom, R., C. J. A. Sol, M. M. M. Salimans, C. J. Jansen, P. M. E. Werlheim-van Dillen, and J. van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503.
2. Boyce, J. M. 1991. Should we vigorously try to contain and control methicillin-resistant Staphylococcus aureus. Infect. Control Hosp. Epidemiol. 12:46-54.
3. Brumfitt, W., and J. M. H. Miller. 1989. Methicillin-resistant Staphylococcus aureus. N. Engl. J. Med. 320:1188-1196.
4. Couto, I., J. Melo-Cristino, M. L. Fernandes, T. Garcia, N. Serrano, M. J. Salgado, A. Torres-Pereira, I. S. Sanches, and H. de Lencastre. 1995. Unusually large number of methicillin-resistant Staphylococcus aureus clones in a Portuguese hospital. J. Clin. Microbiol. 33:2032-2035.
5. De Lencastre, H., I. Couto, I. Santos, J. Melo-Cristino, A. Torres-Pereira, and A. Tomasz. 1994. Methicillin-resistant Staphylococcus aureus disease in a Portuguese hospital: Characterization of clonal types by a combination of DNA typing methods. Eur. J. Clin. Microbiol. Infect. Dis. 13:64-73.
6. Dominguez, M. A., H. d. Lencastre, J. Linares, and A. Tomasz. 1994. Spread and maintenance of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone during an outbreak of MRSA disease in a Spanish hospital. J. Clin. Microbiol. 32:2081-2087.
7. Frenay, H. M., J. P. G. Theelen, L. M. Schouls, C. M. J. E. Vandenbroucke-Grauls, J. Verhoef, W. J. van Leeuwen, and F. R. Mooi. 1994. Discrimination of epidemic and nonepidemic strains of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphisms. J. Clin. Microbiol. 32:846-847.
8. Geha, D. J., J. R. Uhl, C. A. Gustaferro, and D. H. Persing. 1994. Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J. Clin. Microbiol. 32:1768-1772.
9. Goering, R. V. 1993. Molecular epidemiology of nosocomial infection: Analysis of chromosomal restriction fragment patterns by pulsed-field gel electrophoresis. Infect. Control Hosp. Epidemiol. 14:595-600.
10. Kluytmans, J., W. van Leeuwen, W. Goessens, R. Hollis, S. Messer, L. Herwaldt, H. Bruining, M. Heck, J. Rost, N. van Leeuwen, A. v. Belkum, and H. Verbrugh. 1995. Food-initiated outbreak of methicillin-resistant Staphylococcus aureus analyzed by pheno- and genotyping. J. Clin. Microbiol. 33:1121-1128.
11. Kreiswirth, B., J. Kornblum, R. D. Arbeit, W. Eisner, J. N. M. Maslow, A. D. E. Low, and R. P. Novick. 1993. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science 259:227-230.
12. Owen, R. J. 1989. Chromosomal DNA fingerprinting - a new method of species and strain identification applicable to microbial pathogens. J. Med. Microbiol. 30:89-99.
13. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning, a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.