Determination of residues important in hormone binding to the extracellular domain of the luteinizing hormone/chorionic gonadotropin receptor by site-directed mutagenesis and modeling

Molecular Endocrinology

Volumn 10, Issue 9, 1996, Pages 1147-1159

  Bhowmick, Neil ^a   Huang', Jianing ^a,c   Puett, David ^a   Isaacs, Neil W ^b   Lapthorn, Adrian J ^b  

^a University of Georgia   (United States)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

^c AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHORIONIC GONADOTROPIN RECEPTOR; LUTEINIZING HORMONE;

AMINO ACID SEQUENCE; ARTICLE; HORMONE BINDING; LIGAND BINDING; MODEL; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CHORIONIC GONADOTROPIN; CONSERVED SEQUENCE; COS CELLS; DNA, COMPLEMENTARY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN CONFORMATION; RABBITS; RECEPTORS, LH; RECOMBINANT PROTEINS; TRANSFECTION;

SUIDAE;

EID: 0029757509     PISSN: 08888809     EISSN: None     Source Type: Journal    
DOI: 10.1210/me.10.9.1147     Document Type: Article

References (45)

1. Segaloff DL, Ascoli M 1993 The lutropin/choriogonadotropin receptor. . . 4 years later. Endocr Rev 14:324-3472
2. Rosemblit N, Ascoli M, Segaloff DL 1988 Characterization of an antiserum to the rat luteal luteinizing hormone/ chorionic gonadotropin receptor. Endocrinology 123:2284-2290
3. Xie Y-B, Wang H, Segaloff DL 1990 Extracellular domain of lutropin/choriogonado-lutropin receptor expressed in transfected cells binds choriogonadotropin with high affinity. J Biol Chem 265:21411-21414
4. Tsai-Morris CH, Buczko E, Wang W, Dufau ML 1990 Intronic nature of the rat luteinizing hormone receptor gene defines a soluble receptor subspecies with hormone binding affinity. J Biol Chem 265:19385-19388
5. Braun T, Schofield PR, Sprengel R 1991 Amino-terminal leucine-rich repeats in gonadotropin receptors determine hormone selectivity. EMBO J 10:1885-1890
6. Moyle WR, Bernard MP, Myers RV, Marko OM, Strader CD 1991 Lutropin/β adrenergic receptor chimeras bind choriogonadotropin and adrenergic ligands but are not expressed at the cell surface. J Biol Chem 266:10807-10812
7. Ji I, Ji T 1991 Exon 1-10 of the rat LH receptor encode a high affinity hormone binding site and exon 11 encodes a G-protein modulation and a potential second hormone binding site. Endocrinology 128:2648-2650
8. McFarland KC, Sprengel R, Phillips HS, Kohler M, Rosemblit N, Nickolics K, Segaloff DL, Seeburg PH 1989 Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family. Science 245:494-499
9. Loosfelt H, Misrahi M, Atger M, Salesse R, Vu Hai-Luu Thi MT, Jolivet A, Guiochon-Mantel A, Sar S, Jallal B, Garnier J, Milgrom E 1989 Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain. Science 245:525-528
10. Minegishi T, Nakamura K, Takakura Y, Miyamoto K, Hasegaway Y, Ibuki Y, Igarashi M 1990 Cloning and sequencing of the human LH/hCG receptor cDNA. Biochem Biophys Res Commun 172:1049-1054
11. 2+ mobilization. J Biol Chem 267:4479-4488
12. Koo YB, Ji I, Slaughter RG, Ji TH 1991 Structure of the luteinizing hormone receptor gene and multiple exons of the coding sequence. Endocrinology 128:2297-2308
13. Kobe B, Deisenhofer J 1995 A structural basis of the interactions between leucine-rich repeats and protein ligands. Nature 374:183-186
14. Rothberg JM, Goodman CS, Artavanis-Tsakonas S 1990 Slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains. Genes Dev 4:2169-2187
15. Moyle WR, Campbell RK, Rao SNV, Ayad NG, Bernard MP, Han Y, Wang Y 1995 Model of human chorionic gonadotropin and lutropin receptor interaction that explains signal transduction of the glycoprotein hormones. J Biol Chem 270:20020-20032
16. Kajava AV, Vassart G, Wodak S 1995 Modeling of the three-dimensional structure proteins with the typical leucine-rich repeats. Structure 3:867-877
17. Jiang X, Dreano M, Buckler DR, Cheng S, Ythier A, Wu H, Hendrickson WA, Tayar NE 1995 Structural predictions for the ligand binding region of glycoprotein hormone receptors and the nature of hormone receptor interactions. Structure 3:1341-1353
18. Kobe B, Deisenhofer J 1993 Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats. Nature 366:751-756
19. Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Morgan FJ, Isaacs NW 1994 Crystal structure of human chorionic gonadotropin. Nature 369:455-461
20. Wu H, Lustbader JW, Liu Y, Canfield RE, Hendrickson WA 1994 Structure of human chorionic gonadotropin of 26 Å resolution from MAD analysis of the selenomethionyl protein. Structure 2:545-558
21. Sairam MR 1983 Gonadotropic hormones: relationship between structure and function with emphasis on antagonists. Horm Proteins Pept 11:1-78
22. Ryan RJ, Keutmann HT, Chariesworth MC, McCormick DJ, Milius RP, Calvo FO, Vutyavanich, T 1987 Structure-function relationships of gonadotropins. Rec Prog Horm Res 43:83-429
23. Moyle WR, Campbell RK, Myers RV, Bernard MP, Han Y, Wang X 1994 Co-evolution of ligand-receptor pairs. Nature 368:251-255
24. Roche PC, Ryan RJ, McCormick DJ 1992 Identification of hormone-binding regions of the luteinizing hormone/ human chorionic gonadotropin receptor using synthetic peptides. Endocrinology 131:268-274
25. Keutmann HT 1992 Receptor binding regions in human glycoprotein hormones. Mol Cell Endocrinol 86:C1-C6
26. Hipkin RW, Sanchez-Yague J, Ascoli M 1992 Identification and characterization of a luteinizing hormone/chorionic gonadotropin (LH/CG) receptor precursor in a human kidney cell line stably transfected with the rat luteal LH/CG receptor complementary DNA. Mol Endocrinol 6:2210-2218
27. Kobe B, Deisenhofer J 1994 The leucine-rich repeat: a versatile binding motif. Trends Biochem Sci 19:415-421
28. Steinbacher S, Seckler R, Miller S, Steipe B, Huber R, Reinemer P 1994 Crystal structure of P22 tailspike protein: interdigitated subunits in a thermostable trimer. Science 265:383-386
29. Yoder MD, Keen NT, Jurnak F 1993 New domain motif: the structure of pectate lyase C, a secreted plant virilance factor. Science 260:1503-1507
30. Kobe B, Deisenhofer J 1995 Proteins with leucine-rich repeats. Curr Opin Struct Biol 5:409-416
31. Sander C, Schneider R 1991 Database of homology-derived protein structures. Proteins 9:56-68
32. Jones TA, Zou JY, Cowan SW, Kjeldgaard M 1991 Improved methods for building models in electron density maps and location of errors in those models. Acta Crystallogr A 47:110-119
33. Surles MC 1992 An algorithm with linear complexity for interactive, physically-based modeling of large proteins. Comp Graphics 26:221-230
34. Nicholls A, Sharp KA, Honig B 1991 Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins 11:281-296
35. Nothacker H-P, Grimmelikhuijzen CJ-P 1993 Molecular cloning of a novel, putative G protein-coupled receptor from sea anemones structurally related to members of the FSH, TSH, LH/CG receptor family from mammals. Biochem Biophys Res Commun 197:1062-1069
36. Nagayama Y, Wadsworth HL, Russo D, Chazenbalk GD, Rapoport B 1991 Binding domains of stimulatory and inhibitory thyrotropin (TSH) receptor autoantibodies determined with chimeric TSH-lutropin/chorionic gonadotropin receptors. J Clin Invest 88:336-340
37. Huang, J, Puett D 1995 Identification of two amino acid residues in the extracellular domain of the lutropin/choriogonadotropin receptor important in signaling. J Biol Chem 270:30023-30028
38. Liu X, David D, Segaloff DL 1993 Disruption of potential sites for N-linked glycosylation does not impair hormone binding to the lutropin/choriogonadotropin receptor if Asn-173 is left intact. J Biol Chem 268:1513-1516
39. Tapanainen JS, Bo M, Dunkel L, Billig H, Perlas EA, Boime I, Hsueh AJW 1993 Deglycosylation of the human luteinizing hormone receptor does not affect ligand binding and signal transduction. Endocr J 1:219-225
40. Zhang R, Cai H, Fatima N, Buczko E, Dufau ML 1995 Functional glycosylation sites of the rat luteinizing hormone receptor required for ligand binding. J Biol Chem 270:21722-21728
41. Sairam MR 1989 Role of carbohydrates in glycoprotein hormone signal transduction. FASEB J 3:1915-1926
42. Thomas DM, Segaloff DL 1994 Hormone-binding properties and glycosylation pattern of a recombinant form of the extracellular domain of the luteinizing hormone/chorionic gonadotropin receptor expressed in mammalian cells. Endocrinology 135:1902-1912
43. 583 in the third extracellular loop of the lutropin/choriogonadotropin receptor is critical for signaling. J Biol Chem 271:925-930
44. Roche PC, Berger ER, Ryan RJ, 1985 A simple and rapid method using polyethyleneimine-treated filters for assay of solubilized LH/hCG receptors. Endocrinology 117:790-792
45. Jones TA 1982 In: Sayre D (ed) Computational Crystallography. Oxford University Press, Oxford, U.K., 303-317