Application of primary hepatocytes from p53-knockout mice for studies of expression of Cyp3a

Journal of Biochemistry

Volumn 120, Issue 1, 1996, Pages 42-48

  Shimoji, Miyuki ^a   Itoh, Susumu ^a,c   Toide, Kenji ^a,d   Nakayama, Kazuo ^a   Aizawa, Shinichi ^b   Kamataki, Tetsuya ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b KUMAMOTO UNIV SCHOOL OF MEDICINE   (Japan)

^c OSAKA UNIVERSITY   (Japan)

^d DAINIPPON SUMITOMO PHARMA CO LTD   (Japan)

Author keywords

Cyp3a; Dexamethasone; Hepatocytes; p53; Primary culture

Indexed keywords

DEXAMETHASONE; MESSENGER RNA;

ANIMAL CELL; ARTICLE; GENE CONTROL; GENE EXPRESSION; LIVER CELL; MOUSE; NONHUMAN;

ANIMALIA;

EID: 0029756204     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021391     Document Type: Article

References (45)

1. Yanagimoto, T., Itoh, S., Müller-Enoch, D., and Kamataki, T. (1992) Mouse liver cytochrome P-450 (P-450IIIAM1): Its cDNA cloning and inducibility by dexamethasone. Biochim. Biophys. Acta 1130, 329-332
2. 1). Biochim. Biophys. Acta 1201, 405-410
3. Itoh, S., Satoh, M., Abe, Y., Hashimoto, H., Yanagimoto, T., and Kamataki, T. (1994) A novel form of mouse cytochrome P450 3A (Cyp3a-16): Its cDNA cloning and expression in fetal liver. Eur. J. Biochem. 226, 877-882
4. Guillouzo, A., Morel, F., Ratanasavanh, D., Chesne, C., and Guguen-Guillouzo, C. (1990) Long-term culture of functional hepatocytes. Toxic, in vitro 4, 415-427
5. Doostdar, H., Grant, M.E., Melvin, W.T., Wolf, C.R., and Burke, M.D. (1993) The effects of inducing agents on cytochrome P450 and UDP-glucuronyltransferase activities in human HepG2 hepatoma cells. Biochem. Pharmacol. 46, 629-635
6. Schuetz, E.G., Schuetz, J.D., Strom, S.C., Thompson, M.T., Fisher, R.A., Molowa, D.T., and Guzelian, P.S. (1993) Regulation of human liver cytochrome P-450 in family 3A in primary and continuous culture of human hepatocytes. Hepatology 18, 1254-1262
7. Donato, M.T., Bassi, A.M., Gomez-Lechon, M.J., Penco, S., Herrero, E., Adamo, D., Castell, J.V., and Ferro, M. (1994) Evaluation of the xenobiotic biotransformation capability of six rodent hepatoma cell lines in comparison with rat hepatocytes. In Vitro Cell. Dev. Biol. 30A, 574-580
8. Evarts, R.P., Marsden, E., and Thorgeirsson, S.S. (1984) Regulation of heme metabolism and cytochrome P-450 levels in primary culture of rat hepatocytes in a defined medium. Biochem. Pharmacol. 33, 565-569
9. Dich, J., Vind, C., and Grunnet, N. (1988) Long-term culture of hepatocytes: Effects of hormones on enzyme activities and metabolic capacity. Hepatology 8, 39-45
10. Nishibe, Y. and Hirata, M. (1993) Effects of phenobarbital and other model inducers on cytochrome P450 isozymes in primary culture of dog hepatocytes. Xenobiotica 23, 681-692
11. Begue, J.M., Guguen-Guillouzo, C., Pasdeloup, N., and Guillouzo, A. (1984) Prolonged maintenance of active cytochrome P-450 in adult rat hepatocytes co-cultured with another liver type. Hepatology 4, 839-842
12. Kocarek, T.A., Schurtz, E.G., and Guzelian, P.S. (1993) Expression of multiple forms of cytochrome P450 mRNAs in primary culture of rat hepatocytes maintained on Matrigel. Mol. Pharmacol. 43, 328-334
13. Waxman, D.J., Morrissey, J.J., Naik, S., and Jauregui, H.O. (1990) Phenobarbital induction of cytochromes P-450: High- level long-term responsiveness of primary rat hepatocytes cultures to drug induction, and glucocorticoid dependence of the phenobarbital response. Biochem. J. 271, 113-119
14. Koebe, H.G., Pahernik, S., Eyer, P., and Schildberg, F.W. (1994) Collagen gel immobilization: A useful cell culture technique for long-term metabolic studies on human hepatocytes. Xenobiotica 24, 95-107
15. Matsushita, N., Oda, H., Kobayashi, K., Akaike, T., and Yoshida, A. (1994) Induction of cytochrome P-450s and expression of liver-specific genes in rat primary hepatocytes cultured on different extracellular matrices. Biosci. Biotech. Biochem. 58, 1514-1516
16. Nakamura, T., Nawa, K., and Ichihara, A. (1984) Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats. Biochem. Biophys. Res. Commun. 122, 1450-1459
17. Sawada, N., Lee, G.H., Mochizuki, Y., and Ishikawa, T. (1988) Active proliferation of mouse hepatocytes in primary culture under defined conditions as compared to rat hepatocytes. Jpn. J. Cancer Res. (Gann) 79, 983-988
18. Bissell, D.M. and Guzelian, P.S. (1979) Ascorbic acid deficiency and cytochrome P450 in adult rat hepatocytes in primary monolayer culture. Arch. Biochem. Biophys. 192, 569-576
19. Mitaka, T., Norioka, K., and Mochizuki, Y. (1993) Redifferentiation of proliferated rat hepatocytes cultured in L15 medium supplemented with EGF and DMSO. In Vitro Cell. Dev. Biol. 29A, 714-722
20. Nemoto, N. and Sakurai, J. (1993) Activation of Cyp1a1 and Cyp1a2 genes in adult mouse hepatocytes in primary culture. Jpn. J. Cancer Res. (Gann) 84, 272-278
21. Kremers, P., Roeland, L., Stouvenakers, N., Goffinet, G., and Thome, J.P. (1994) Expression and induction of drug-metabolizing enzymes in cultured fetal rat hepatocytes. Cell Biol. Toxicol. 10, 117-125
22. Nemoto, N., Sakurai, J., and Funae, Y. (1995) Maintenance of phenobarbital-inducible Cyp2b gene expression in C57BL/6 mouse hepatocytes in primary culture as spheroids. Arch. Biochem. Biophys. 316, 362-369
23. Donehower, L.A. and Bradley, A. (1993) The tumor suppressor p53. Biochim. Biophys. Acta 1155, 181-205
24. Jenkins, J.R., Rudge, K., and Currie, G.A. (1984) Cellular immortalization by a cDNA clone encoding the transformation- associated phosphoprotein p53. Nature 312, 651-654
25. Yanai, N., Suzuki, M., and Obinata, M. (1991) Hepatocyte cell lines established from transgenic mice harboring temperature- sensitive simian virus 40 large T-antigen gene. Exp. Cell. Res. 197, 50-56
26. Woodworth, C.D. and Isom, H.C. (1987) Regulation of albumin gene expression in a series of rat hepatocyte cell lines immortal- ized by simian virus 40 and maintained in chemically defined medium. Mol. Cell. Biol. 7, 3740-3748
27. Pfeifer, A.M.A., Cole, K.E., Smoot, D.T., Weston, A., Groopman, J.D., Shields, P.G., Vignaud, J.-M., Juillerat, M., Lipsky, M.M., Trump, B.F., Lechner, J.F., and Harris, C.C. (1993) Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90, 5123-5127
28. Wu, J.C., Merlino, G., and Fausto, N. (1994) Establishment and characterization of differentiated, nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor α. Proc. Natl. Acad. Sci. USA 91, 674-678
29. Mead, J.E. and Fausto, N. (1989) Transforming growth factor α may be a physiological regulator of liver regeneration by means of an autocrine mechanism. Proc. Natl. Acad. Sci. USA 86, 1558- 1562
30. Fabregat, I., Juan, C.D., Nakamura, T., and Benito, M. (1992) Growth stimulation of rat fetal hepatocytes in response to hepatocyte growth factor: Modulation of c-myc and c-fos expression. Biochem. Biophys. Res. Commun. 189, 684-690
31. Mercer, W.E., Shields, M.T., Amin, M., Sauve, G.J., Appella, E.A., Romano, J.W., and Ulrich, S.J. (1990) Negative growth regulation in a glioblastoma tumor cell line that conditionally expresses human wild-type p53. Proc. Natl. Acad. Sci. USA 87, 6166-6170
32. Baker, S.J., Markowitz, S., Feron, E.R., Wilson, J.K.V., and Vogelstein, B. (1990) Suppression of human colorectal carcinoma cell growth by wild-type p53. Science 249, 912-915
33. Donehower, L.A, Harvey, M., Slagle, B.L., McArthur, M.J., Montgomery, C.A., Jr., Butel, J.S., and Bradley, A. (1992) Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. Nature 356, 215-221
34. Tsukada, T., Tomooka, Y., Takai, S., Ueda, Y., Nishikawa, S., Yagi, T., Tokunaga, T., Takeda, N., Suda, Y., Abe, S., Matsuo, I., Ikawa, Y., and Aizawa, S. (1993) Enhanced proliferative potential in culture of cells from p53-deficient mice. Oncogene 8, 3313- 3322
35. Harvey, M., Sands, A.T., Weiss, R.S., Hegi, M.E., Wiseman, R.W., Pantazis, P., Giovanella, B.C., Tainsky, M.A., Bradley, A., and Donehower, L.A. (1993) In vitro growth characteristics of embryo fibroblasts isolated from p53-deficient mice. Oncogene 8, 2457-2467
36. Sawada, N., Tonomura, A., Sattler, C.A., Sattler, G.L., Kleinman, H.K., and Pilot, H.C. (1987) Effects of extracellular matrix components on the growth and differentiation of cultured rat hepatocytes. In Vitro Cell. Dev. Biol. 23, 267-273
37. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol chloroform extraction. Anal. Biochem. 162, 156-159
38. Alonso, S., Minty, A., Bourlet, Y., and Buckingham, M. (1986) Comparison of three actin-coding sequences in the mouse: Evolutionary relationships between the actin genes of warm- blood vertebrates. J. Mol. Evol. 23, 11-22
39. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning: A Laboratory Manual, 2nd ed., p. 7.36. Cold Spring Harbor Laboratory, Cold Spring Harbor
40. Aitio, A. (1978) A simple and sensitive assay of 7-ethoxycoumarin deethylation. Anal. Biochem. 85, 488-491
41. Yamazoe, Y., Murayama, N., Shimada, M., Yamauchi, K., Nagata, K., Imaoka, S., Funae, Y., and Kato, R. (1988) A sex-specific form of cytochrome P-450 catalyzing propoxycoumarin O-depropylation and its identity with testosterone 6β- hydroxylase in untreated rat livers: Reconstitution of the activity with microsomal lipids. J. Biochem. 104, 785-790
42. Nemoto, N. and Sakurai, J. (1995) Glucocorticoid and sex hormones as activating or modulating factors for expression of Cyp2b-9 and Cyp2b-10 in the mouse liver and hepatocytes. Arch. Biochem. Biophys. 319, 286-292
43. Sinclair, P.R., Bement, W.J., Haugen, S.A., Sinclair, J.F., and Guzelian, P.S. (1990) Induction of cytochrome P-450 and 5- aminolevulinate synthase activities in cultured rat hepatocytes. Cancer Res. 50, 5219-5224
44. Aoyama, T., Korzekwa, K., Matsunaga, T., Nagata, K., Gillette, J., Gelboin, H.V., and Gonzalez, F.J. (1990) cDNA-directed expression of rat P450s IIA1 and IIA2, catalytic activities toward steroids and xenobiotics and comparison with the enzymes purified from liver. Drug Metab. Dispos. 18, 378-382
45. Shiota, G., Rhoads D.B., Wang, T.C., Nakamura, T., and Schmidt, E.V. (1992) Hepatocyte growth factor inhibits growth of hepatocellular carcinoma cells. Proc. Natl. Acad. Sci. USA 89, 373-377