Fluorescence spectral properties of troponin C mutant F22W with one-, two-, and three-photon excitation

Biophysical Journal

Volumn 71, Issue 6, 1996, Pages 3448-3453

  Gryczynski, Ignacy ^a   Malak, Henryk ^a   Lakowicz, Joseph R ^a   Cheung, Herbert C ^b   Robinson, John ^b   Umeda, Patrick K ^c  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c Department of Medicine ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PROTEIN; TROPONIN C; TRYPTOPHAN;

ANIMAL TISSUE; ANISOTROPY; ARTICLE; CHICKEN; FLAME PHOTOMETRY; MUTANT; NONHUMAN; PHOTON; SITE DIRECTED MUTAGENESIS; SPECTROFLUOROMETRY; STEADY STATE;

ANIMALIA; GALLUS GALLUS;

EID: 0029754663     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79540-1     Document Type: Article

References (25)

1. Beechem, J. M., and L. Brand. 1985. Time-resolved fluorescence of proteins. Annu. Rev. Biochem. 54:43-71.
2. Demchenko, A. P. 1992. Fluorescence and dynamics in proteins. In Topics in Fluorescence Spectroscopy, Vol. 3: Biochemical Applications. Plenum Press, New York. 65-111.
3. Denk, W., K. R. Delaney, A. Gelperin, D. Kleinfeld, B. W. Strowbridge, D. W. Tank, and R. Yuste. 1994. Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy. J. Neurosci. Methods. 54:151-152.
4. Denk, W., D. W. Piston, and W. W. Webb. 1995. Two-photon molecular excitation in laser-scanning microscopy. In Book of Biological Confocal Microscopy. J. B. Pawley, editor. Plenum Press, New York. 445-458.
5. Denk, W., J. H. Strickler, and W. W. Webb. 1990. Two-photon laser scanning fluorescence microscopy. Science. 248:73-76.
6. b emission in 5-methylindole. J. Fluoresc. 4:165-168.
7. b transitions and comparison with theory. J. Phys. Chem. 94:3469-3479.
8. Gratton, E., J. R. Lakowicz, B. Maliwal, H. Cherek, G. Laczko, and M. Limkeman. 1984. Resolution of mixtures of fluorophores using variable-frequency phase and modulation data. Biophys. J. 46:479-486.
9. Herzberg, O., J. Moult, and M. N. G. James. 1986. Structure of the calcium regulatory muscle protein troponin C at 2.9 Å resolution. J. Biol. Chem. 261:2638-2644.
10. Jameson, D. M., and G. D. Reinhart, editors. 1989. Fluorescent Biomolecules, Methodologies and Applications. Plenum Press, New York. 461.
11. Konev, S. V., editor. 1967. Fluorescence and Phosphorescence of Proteins and Nucleic Acids. Plenum Press, New York. 204.
12. Laczko, G., J. R. Lakowicz, I. Gryczynski, Z. Gryczynski, and H. Malak. 1990. A 10 GHz frequency-domain fluorometer. Rev. Sci. Instrum. 61:2331-2337.
13. Lakowicz, J. R., H. Cherek, J. Kuśba, I. Gryczynski, and M. L. Johnson. 1993. Review of fluorescence anisotropy decay analysis by frequency-domain fluorescence spectroscopy. J. Fluoresc. 3:103-116.
14. Lakowicz, J. R., E. Gratton, G. Laczko, H. Cherek, and M. Limkeman. 1984. Analysis of fluorescence decay kinetics from variable-frequency phase shift and modulation data. Biophys. J. 46:463-477.
15. Lakowicz, J. R., and I. Gryczynski. 1992. Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation. J. Fluoresc. 2:117-122.
16. Lakowicz, J. R., and I. Gryczynski. 1993. Tryptophan fluorescence intensity and anisotropy decays of human serum albumin resulting from one-photon and two-photon excitation. Biophys. Chem. 45:1-6.
17. Lakowicz, J. R., I. Gryczynski, E. Danielsen, and J. K. Frisoli. 1992a. Unexpected anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence. Chem. Phys. Lett. 194:282-287.
18. Lakowicz, J. R., I. Gryczynski, J. Kuśba, and E. Danielsen. 1992b. Two photon-induced fluorescence intensity and anisotropy decays of diphenylhexatriene in solvents and lipid bilayers. J. Fluoresc. 2:247-258.
19. Lakowicz, J. R., and B. P. Maliwal. 1985. Construction and performance of a variable-frequency phase-modulation fluorometer. Biophys. Chem. 21:61-78.
20. Lindek, S., E. H. K. Stelzer, and S. W. Hell. 1995. Two new high-resolution confocal fluorescence microscopies (4PI, Theta) with one-and two-photon excitation. In Book of Biological Confocal Microscopy. J. B. Pawley, editor. Plenum Press, New York. 632.
21. Rehms, A. A., and P. R. Callis. 1993. Two-photon fluorescence excitation spectra of aromatic amino acids. Chem. Phys. Lett. 208:276-282.
22. She, M., W.-J. Dong, H. C. Cheung, and P. K. Umeda. 1996a. A time-resolved study of the fluorescence of troponin C mutant F22W. Biophys. J. 70(2, pt. 2):A38.
23. She, M., W.-J. Dong, P. Umeda, and H. C. Cheung. 1996b. Tryptophan mutants of troponin C from skeletal muscle. An optical probe of the regulatory domain. J. Biol. Chem. submitted.
24. b excitation bands. Photochem. Photobiol. 25:441-444.
25. Weber, G. 1960. Fluorescence-polarization spectrum and electronic-energy transfer in tyrosine, tryptophan and related compounds. Biochem. J. 75:335-345.