Localization of α1,2,3-subunit isoforms of Na,K-ATPase in cultured neonatal and adult rat myocardium: The immunofluorescence and immunocytochemical study

Molecular and Cellular Biochemistry

Volumn 163-164, Issue , 1996, Pages 39-45

  Slezak, Jan ^a,d   Schulze, Wolfgang ^b   Stefankova, Zuzana ^a   Okruhlicova, Ludmila ^a   Danihel, Ludovit ^c   Wallukat, Gerd ^b  

^a INSTITUTE OF CHEMISTRY   (Slovakia)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c Physics and Informatics   (Slovakia)

^d Institute for Heart Research   (Slovakia)

Author keywords

Immunoelectron microscopy; Immunofluorescence; K ATPase; Localization; Na

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); DIAMINOBENZIDINE; MONOCLONAL ANTIBODY; PEROXIDASE; POLYCLONAL ANTIBODY;

ACTION POTENTIAL; ANIMAL CELL; ARTICLE; CELL AGING; CONTROLLED STUDY; CYTOCHEMISTRY; ENZYME LOCALIZATION; ENZYME SUBUNIT; HEART CELL CULTURE; HEART MUSCLE CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; NEWBORN; NEWBORN PERIOD; NONHUMAN; RAT; SARCOLEMMA; SARCOPLASMIC RETICULUM;

ANIMALIA; STROPHANTHUS GRATUS;

EID: 0029752808     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00408639     Document Type: Article

References (32)

1. Skou JC: The influence of some cations on the adenosine triphosphatase from peripheral nerves. Biochem Biophys Acta 23: 394-401, 1957
2. ++-abh'ängigen Adenosintriphosphat Phosphohydrolase-Systems des Herzmuskels durch Isolierung der Zellmembran. Acta biol med germ 19: 879-906, 1961
3. Repke KRH: Uber den biochemischen Wirkungsmodus von Digitalis. Klin Wschr 42: 157-165, 1964
4. +-abhängigen glykosidempfmdlichen Adenosintriphosphatase des Herzmuskels an der Plasmamembran des Sarkolemms. Naturwissenschaften 53: 134, 1966
5. +- und anderer membranständiger ATPase-Aktivität im Herzmuskel. Histochemie 19: 302-318, 1969
6. Urayama O, Shutt H, Sweadner KJ: Identification of three isoenzyme proteins of the catalytic subunit of the Na,K-ATPase in rat brain. J Biol Chem 264: 8271-8280, 1989
7. Martin-Vasallo P, Dackowski W, Emanuel JR, Levenson R: Identification of a putative isoform of the Na,K-ATPase β subunit. Primary structure and tissue-specific expression. J Biol Chem 264: 4613-4618, 1989
8. DeTomaso AW, Blanco G, Mercer RW: The α and β subunits of the Na,K-ATPase can assemble at the plasma membrane into functional enzyme. J Cell Biol 127: 55-69, 1994
9. Shyjan AW, Cena V, Klein DC, Levenson R: Differential expression and enzymatic properties of the Na,K-ATPase α3 isoenzyme in rat pineal glands. Proc Natl Acad Sci USA 87: 1178-1182, 1990
10. Shyjan AW, Gottardi C, Levenson R: The Na,K-ATPase, β2 subunit is expressed in rat brain and copurifies with Na,K-ATPase activity. J Biol Chem 265: 5166-5169, 1990
11. Sweadner KJ, Gilkeson RC: Two isoenzymes of the Na,K-ATPase have distinct antigenic determinants. J Biol Chem 260: 9016-9022, 1985
12. Arystarkhova E, Gasparian M, Modyanova NN, Sweadner KJ: Na,K-ATPase extracellular surface probed with a monoclonal antibody that enhances ouabain binding. J.Biol Chem 267: 13694-13701, 1992
13. Jaisser F, Canessa CM, Horisberger JD, Rossier BC: Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase - the beta subunit modulates potassium activation of the Na,K-pump. J Biol Chem 267: 16895-1690, 1992
14. Shyjan AW, Levenson R: Antisera specific for the α1, α2, α3, and β-subunits of the Na,K-ATPase: Differential expression of α and β subunits in rat tissue membranes. Biochemistry 28: 4531-4535, 1989
15. Felsenfeld DP, Sweadner KJ: Fine specificity mapping and topography of an isoenzyme-specific epitope oftheNa,K-ATPase catalyticunit. J Biol Chem 236: 10932-10942, 1988
16. Kent RL, Hoober JK, Cooper G: Load responsiveness of protein synthesis in adult mammalian myocardium: role of cardiac deformation linked to sodium influx. Circ Res 64: 74-85, 1989
17. Halle W, Wollenberger A: Differentiation and behavior of isolated embryonic and neonatal heart cells in a chemically defined medium. Am J Cardiol 25: 292-299, 1970
18. Laemmli N.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680-685, 1970
19. Klip A., Ramal T., Young DA, Holloszy JO: Insulin-induced translocation of glucose transporters in rat hindlimb muscles. FEBS Letters 224: 224-230, 1987
20. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 71: 248-254, 1976
21. Pederson P, Carafoli E: Ion motive ATPases. II. Energy coupling and work output. Trends Biochem Sci 12: 186-189, 1987
22. Sweadner KJ, Herera VLM, Amato S, Moellman A, Gibbons DK, Repke KRH: Immunologic identification of Na,K-ATPase isoforms in myocardium. Circ Res 74: 669-678, 1994
23. Lucchesi PA, Sweadner KJ: Postnatal changes in Na,K-ATPase isoform expression in rat cardiac ventricle. J. Biol Chem. 266: 9327-9331, 1991
24. Orlowski J, Lingrel JB: Tissue specific and developmental regulation of rat Na,K-ATPase catalytic a isoform and, β-subunit mRNAs. J Biol Chem 263: 10436-10442, 1988
25. Zahler R., Gilmore-Hebert M, Baldwin JC, Franco K, Benz EJ: Expression of a isoforms of the Na,K-ATPase in human heart. Biochim Biophys Acta 1149: 189-194, 1993
26. Cohen NM, Lederer WJ: Changes in the calcium current of rat heart ventricular myocytes during development. J Physiol 406: 115-146, 1988
27. Asano G, Ashraf M, Schwartz A: Localization of Na,K-ATPase in guinea-pig myocardium. J Mol Cell Cardiol 12: 257-266, 1980
28. Wollenberger A, Schulze W: Cytochemical studies on sarcolemma: Na,K-adenosine triphosphatase and adenylate cyclase. In: Recent Advances in Studies on Cardiac Structure and Metabolism 9: P.-E. Roy, N.S. Dhalla (eds). University Park Press, Baltimore, Maryland, 1976, pp. 101-115
29. Slezak J, Okruhlicova L, Tribulova N, Schulze W, Dhalla NS: Renaissance of cytochemical localization of ion-transport ATPases in the myocardium. Mol Cell Biochem 147: 169-172, 1995
30. Noel F, Wibo M, Godfraind T: Distribution of α1 and α2 Na,K-ATPase isoforms between the junctional (t-tubular) and nonjunctional sarcoplasmic domains of rat ventricle. Biochem Pharmacol 41: 313-315, 1991
31. Hool LC, Whalley DW, Doohan MM, Rasmussen HH: Angiotensin-converting enzyme inhibition, intracellular Na+, and Na,K pumping in cardiac myocytes. Am J Physiol 268: C366-C375, 1995
32. Winston D, Spinale FG, Crawford FA, Schulte BA: Immunocytochemical and enzyme histochemical localization of Na,K-ATPase in normal and ischemic porcine myocardium. J Mol Cell Cardiol 22: 1071-1082, 1990