Calcification in hermatypic and ahermatypic corals

Science

Volumn 271, Issue 5249, 1996, Pages 637-639

  Marshall, A T ^a  

^a LA TROBE UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AHERMATYPIC CORAL; CALCIFICATION; CORAL; CORAL REEF; HERMATYPIC CORAL; LIGHT ENHANCEMENT; SYMBIOTIC ALGAE; ZOOXANTHELLA;

AUSTRALIA, GREAT BARRIER REEF, HERON ISLAND;

GALAXEA FASCICULARIS; TUBASTREA FAULKNERI;

EID: 0029752514     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5249.637     Document Type: Article

References (29)

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7. T. G. Jacques, M E Q. Pilson, C Cummings, N. Marshall, in Proceedings of the Third International Coral Reef Symposium, University of Miami, May 1977, D. L. Taylor, Ed (Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL, 1977), pp 455-461, T. G. Jacques, N Marshall, M E. Q Pilsen, Mar. Biol. 76, 135 (1983).
8. -1 The incubation medium was stirred by aeration, and the jars were maintained at constant temperature by immersion in a large aquarium with flow-through seawater at the same temperature as that of reef seawater
9. 45Ca incorporation were obtained by scintillation counting (with a Beckman LS6800 with H number quench correction) of aliquots (200 μl in 5 ml of Beckman Ready-Solv GP scintillation fluid) of solutions of skeleton digested with concentrated HCl after rapid removal of tissue (10 min at 58°C) with 12% sodium hypochlorite solution (bleach). Before this treatment, polyps were vigorously rinsed in seawater for 5 min They were cut into pieces, and any part of the corallite that was not covered by epithelium was discarded, thereby eliminating skeleton in which isotopic exchange may have taken place
10. -1.
11. J. H Vanderrneulen, N. D. Davis, L. Muscatine, Mar. Biol 16, 185 (1972).
12. 45Ca (7), polyps were removed from the rinse water and shaken until no more fluid dripped from them. The polyps were then weighed, treated with bleach, and reweighed after overnight drying at 60°C. This procedure allowed approximate wet tissue mass to be calculated in addition to skeletal mass. Wet tissue mass was compared with tissue protein concentration measured by Peterson's modification of the Lowery method [G. L. Peterson, Anal. Biochem. 83, 346 (1977)] after digestion of the tissues of Galaxea polyps in 5N sodium hydroxide A linear relation between wet mass and protein concentration was obtained (r = 0.8, n = 18). Protein concentration in Tubastrea tissues could not be measured because the tissue digest was strongly colored.
13. 45Ca activity in the incubation seawater but have not been normalized with respect to time. The variation in incubation times (3 to 4 hours in Figs. 1 and 2,1 hour in Fig 3) largely accounts for the differences in control values, although these are not intended to be compared because experimental conditions were not identical.
14. B. E Chalker, Comp. Biochem. Physiol. 54A, 455 (1976).
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17. 2+ exchange
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21. -2. Dark polyps were in a jar shrouded by black plastic
22. A T Marshall and A Wright, in preparation
23. -1.
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26. T. McConnaughey, in Origin, Evolution and Modem Aspects of Biomineralisation in Plants and Animals, R. E. Crick, Ed (Plenum, New York, 1989), pp 57-73
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28. D. J. Barnes and C. J. Crossland, Limnol. Oceanogr. 25, 113 (1980).
29. I thank I. Lawn for facilities at Heron Island Research Station Corals were collected under permit no. G91/353 from the Great Barrier Reef Marine Parks Authority.