Function of myosin-V in filopodial extension of neuronal growth cones

Science

Volumn 273, Issue 5275, 1996, Pages 660-663

  Wang, Feng Song ^a   Wolenski, Joseph S ^b   Cheney, Richard E ^b,c   Mooseker, Mark S ^b   Jay, Daniel G ^a  

^a HARVARD UNIVERSITY   (United States)

^b YALE UNIVERSITY   (United States)

^c UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; MYOSIN;

ACTIN POLYMERIZATION; ANIMAL CELL; ANIMAL TISSUE; CHICKEN; CHROMATOPHORE; CYTOSKELETON; GROWTH CONE; LASER; MOTOR ACTIVITY; NONHUMAN; PRIORITY JOURNAL; REVIEW; SPINAL GANGLION;

ANIMALIA; GALLUS GALLUS;

EID: 0029750192     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5275.660     Document Type: Review

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30. Sliding actin filament assay was done essentially as described (13). Briefly, myosin-V (25 μg/ml) was perfused into motility chambers and allowed to absorb onto the nitrocellulose cover slips for 10 min. After rinses with blocking buffer to remove unbound myosins, MG-labeled antibodies (47.5 μg/ml; molar ratio myosin-V:IgG = 1:7) were added to the chamber and incubated for 30 min. After the removal of unbound antibodies, half of the motility chambers were marked with ink and subjected to laser irradiation for 5 min. After the addition and examination of actin filaments (rhodamine-phalloidin F-actin; 12 nM) in the absence of ATP, sliding was initiated by the addition of motility buffer containing 2 mM ATP. The density of bound actin filaments was indistinguishable between laser-irradiated and unirradiated areas before the initiation of motility. After the addition of ATP, most of the filaments inside the irradiated area were released intact, although a few exhibited Brownian motion and detached gradually. Motility was recorded with a Nikon Diaphot 300 microscope equipped with epifluorescence optics (Plan Apo 100X NA 1.4 oil-immersion objective) and a Dage-MTI SIT camera. Time-lapse images were digitized with MetaMorph image acquisition software (Universal Images, PA) and then transferred to an optical laser disc recorder. Motility assays were done at 24° ± 1°C. Ayttrium-aluminum-garnet-Nd (YAG-Nd)-pumped dye laser (models GCR11 and PDL2, Spectra Physics) was used to generate a 620-nm light at an energy output of 18 mJ per pulse (2-mm-diameter laser spot).
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44. We thank A. Xu for technical assistance and J. P. Albanesi (University of Texas Southwestern Medical Center, Dallas) for providing monoclonal antibodies to myosin-Iβ (M2 and M3). Supported in part by National Institutes of Health (NIH) grants NS-29007 and NS-34699 (to D.G.J.) and DK-25387 (to M.S.M.), the Lucille P. Markey Charitable Trust and Klingenstein fellowship (to D.G.J.), and the Pew Charitable Trust (to J.S.W.). F.-S.W. acknowledges receipt of a postdoctoral fellowship from the NIH.