Rapid automated combined in situ hybridization and immunohistochemistry for sensitive detection of cytomegalovirus in paraffin-embedded tissue biopsies

American Journal of Clinical Pathology

Volumn 106, Issue 4, 1996, Pages 544-548

  Rimsza, Lisa M ^a   Vela, Elizabeth E ^a   Frutiger, Yvette M ^a   Rangel, Catherine S ^a   Solano, Max ^b   Richter, Lynne C ^a   Grogan, Thomas M ^a,c   Bellamy, William T ^a  

^a UNIVERSITY OF ARIZONA   (United States)

^b BAYLOR UNIVERSITY MEDICAL CENTER   (United States)

^c UNIVERSITY OF ARIZONA COLLEGE OF MEDICINE   (United States)

Author keywords

CMV; Immunohistochemistry; In Situ Hybridization

Indexed keywords

ARTICLE; AUTOMATION; BIOPSY; CLINICAL ARTICLE; COHORT ANALYSIS; CONTROLLED STUDY; CYTOMEGALOVIRUS; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; PRIORITY JOURNAL; VIRUS DETECTION;

EID: 0029748323     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1093/ajcp/106.4.544     Document Type: Article

References (17)

1. Meyers J, Flournoy N, Thomas ED. Risk factors for cytomegalovirus infection after human bone marrow transplantation. J Infect Dis 1986;153:478-488.
2. Goodrich JM, Motomi M, Gleaves CA, et al. Early treatment with ganciclovir to prevent cytomegalovirus disease after allogeneic bone marrow transplantation. N Engl J Med 1991;325:1601-1607.
3. Hackman R, Wolford J, Gleaves C, et al. Recognition and rapid diagnosis of upper gastrointestinal cytomegalovirus infection in marrow transplant recipients. Transplantation 1994;5:231-237.
4. Langford A, Kunze R, Schmelzer S, et al. Immunocytochemical detection of herpes viruses in oral smears of HIV-infected patients. J Oral Pathol Med 1992;21:49-56.
5. Sano N, Keisuke I. Hepatic cytomegalovirus involvement in autopsy cases. Acta Pathol Jpn 1991;41:668-672.
6. Masih A, Woods G, Thiele G, et al. Detection of cytomegalovirus in bronchoalveolar lavage: A comparison of techniques. Mod Pathol 1991;4:108-112.
7. Pacchioni D, Papotti M, Bonino F, Bussolati G, Negro F. Detection of cytomegalovirus by in situ hybridization using a digoxigenin-tailed oligonucleotide. Liver 1992;12:257-261.
8. Mougin C, Bassignot A, Coaquette A, Bourgeois A, Lab M. Optimization of in situ hybridization for detection of viral genomes in cultured cells on 96-microwell plates: A cytomegalovirus model. J Clin Micro 1991;29:1735-1739.
9. Bajanowski T, Wiegand P, Brinkmann B. Comparison of different methods for CMV detection. Int J Legal Med 1994;106:219-222.
10. Nadasy T, Miller K, Johnson L, et al. Is cytomegalovirus associated with renal disease in AIDS patients? Mod Pathol 1992;5:277-282.
11. Grogan TM, Rangel C, Rimsza LM, et al. Kinetic-mode, automated double-labelled immunohistochemistry and in situ hybridization in diagnostic pathology. Advances in Pathology and Laboratory Medicine 1995;8:79-100.
12. Smith TF, Espy MJ, Wilson DJ, Wold AD. Monoclonal antibodies for rapid detection of viral antigens in shell vials. Clinical Immunology Newsletter 1987;8:43.
13. Einsele G, Ehninger H, Herbart H. Polymerase chain reaction monitoring reduces the incidence of cytomegalovirus disease and the duration and side effects of antiviral therapy after bone marrow transplantation. Blood 1995;86:2815-2820.
14. Genta RM, Bleyzer I, Cate TR, Tandon AK, Yoffe B. In situ hybridization and immunohistochemical analysis of cytomegalovirus-associated ileal perforation. Gastroenterology 1993;104: 1822-1827.
15. Zipeto D, Revello MG, Silini E, et al. Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocomprmised patients. J Clin Microbiol 1992;30:527-530.
16. Brainaird JA, Greenson JK, Vesy CJ, et al. Detection of cytomegalovirus in liver transplant biopsies. Transplantation 1994;57: 1753-1757.
17. Ho M. Cytomegalovirus. In: Mandell GL, Douglass RG, Bennett JE, eds. Principles and Practice of Infectious Disease. ed 3. New York: Churchill Livingstone, 1990, pp 1159-1172.