Enzymatic activities of several K108 mutants of ribonuclease (RNase) Rh isolated from Rhizopus niveus

Biological and Pharmaceutical Bulletin

Volumn 19, Issue 8, 1996, Pages 1080-1082

  Ohgi, Kazuko ^a   Iwama, Masanori ^a   Ogawa, Yuko ^a   Hagiwara, Chinatsu ^a   Ono, Eriko ^a   Kawaguchi, Ryoko ^a   Kanazawa, Chiharu ^a   Irie, Masachika ^a  

^a HOSHI UNIVERSITY   (Japan)

Author keywords

base non specific ribonuclease; enzymatic action mechanism; lysine residue; Rhizopus niveus; ribonuclease

Indexed keywords

LYSINE; RIBONUCLEASE; SERINE; THREONINE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME MECHANISM; MICHAELIS CONSTANT; NONHUMAN; RHIZOPUS; SACCHAROMYCES;

EID: 0029745218     PISSN: 09186158     EISSN: None     Source Type: Journal    
DOI: 10.1248/bpb.19.1080     Document Type: Article

References (26)

1. Abbreviations and nomenclature: RNase RNAP Rh, RNase Rh with an extra 3 amino acid residues, Ala-Ser-Gly, at the N-terminus. Nomenclature of the mutant enzyme produced by site-directed mutagenesis of RNase RNAP Rh: Mutant enzymes whose amino acid at the ith residue (X) is substituted by Y as a result of site-directed mutagenesis are abbreviated as XiY. Thus K108T is a mutant enzyme in which the lysine residue at the 108th position has been replaced by threonine.
2. Horiuchi H., Yanai K., Takagi M., Yano K., Wakabayashi E., Sanda A., Mine M., Ohgi K., Irie M., J. Biochem. (Tokyo), 103, 408-418 (1989).
3. Kawata Y., Sakiyama F., Tamaoki T., Eur. J. Biochem.. 176, 683-697 (1989).
4. Watanabe H., Naitoh A., Suyama Y., Inokuchi N., Shimada H., Koyama T., Ohgi K., Irie M., J. Biochem. (Tokyo), 108, 303-310 (1990).
5. Inada Y., Watanabe H., Ohgi K., Irie M., J. Biochem. (Tokyo), 110, 896-904 (1990).
6. Kobayashi (Shimada) H., Inokuchi N., Koyama T., Watanabe H., Iwama M., Ohgi K., Irie M., Biosci. Biotech. Biochem., 56, 2003-2010 (1992).
7. Inokuchi N., Koyama T., Sawada F., Irie M., J. Biochem. (Tokyo), 113, 425-430 (1993).
8. McClure B. A., Haring V., Ebert P. R., Andersen M. A., Simpson R. J., Sakiyama F., Clarke A. E., Nature (London), 342, 955-957 (1989).
9. Ai Y., Singh A., Coleman C. E., Ioerger T. P., Sex Plant Reprod., 3, 130-138 (1990).
10. Ide H., Kimura M., Arai M., Funatsu G., FEBS Lett., 284, 161-164 (1991).
11. Jost W., Bak H., Glund K., Terpstra P., Beintema J. J., Eur. J. Biochem., 198, 1-6 (1991).
12. Watanabe H., Narumi H., Inaba T., Ohgi K., Irie M., J. Biochem. (Tokyo), 114, 800-807 (1993).
13. Ohgi K., Horiuchi H., Watanabe H., Takagi M., Yano K., Irie M., J. Biochem. (Tokyo), 109, 775-783 (1991).
14. Ohgi K., Horiuchi H., Iwama M., Watanabe H., Takagi M., Irie M., J. Biochem. (Tokyo), 112, 132-138 (1992).
15. Ohgi K., Horiuchi H., Watanabe H., Iwama M., Takagi M., Irie M., J. Biochem. (Tokyo), 113, 219-224 (1993).
16. Irie M., Ohgi K., Watanabe H., Iwama M., Nakamura K. T., Kurihara H., Nonaka T., Mitsui Y., Horiuchi H., Takagi M., J. Biochem. (Tokyo), 114, 1083-1987 (1994).
17. Ohgi K., Iwama M., Tada K., Takizawa R., Irie M., J. Biochem. (Tokyo), 117, 29-33 (1995).
18. Kurihara H., Mitsui Y., Ohgi K., Irie M., Mizuno H., Nakamura K. T., FEBS Lett., 306, 189-192 (1992).
19. Nakamura T. K., Ishikawa N., Hamashima M., Kurihara H., Nonaka T., Mitusi Y., Ohgi K., Irie M., Abstracts of Papers, The 3rd International Meeting on Ribonuclease, Chemistry, Biology, Biotechnology, Capri, Italy, May 1993, p. 56.
20. Douzou P., Petzko G. A., Adv. Protein Chem., 36, 245-361 (1984).
21. Kunkel T. A., Roberts J. D., Zakau R. A., "Methods in Enzymol ogy," Vol. 154, ed. by Wu R., Grossman L., Academic Press, New York, 1987, pp. 415-123.
22. Sanger F., Coulson A. R., J. Mol. Biol., 94, 441-448 (1975).
23. Laemmli U. K., Nature (London), 227, 686 (1970).
24. Imazawa M., Irie M., Ukita T., J. Biochem. (Tokyo), 64, 556-568 (1968).
25. Witzel H., Barnard E. A., Biochem. Biophys. Res. Commun., 7, 295-299 (1962).
26. Lineweaver M., Burk B., J. Am. Chem. Soc., 56, 658-662 (1934).