Multi-facility survey of oligonucleotide synthesis and an examination of the performance of unpurified primers in automated DNA sequencing

BioTechniques

Volumn 21, Issue 4, 1996, Pages 680-685

  Pon, R T ^g   Buck, G A ^a,h   Hager, K M ^b   Naeve, C W ^c   Niece, R L ^d   Robertson, M ^e   Smith, A J ^f  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b YALE UNIVERSITY   (United States)

^c ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e UNIVERSITY OF UTAH   (United States)

^f STANFORD UNIVERSITY MEDICAL CENTER   (United States)

^g UNIVERSITY OF CALGARY   (Canada)

^h VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE; PRIMER DNA;

ACCURACY; ANALYTIC METHOD; DNA PURIFICATION; DNA SYNTHESIS; HEALTH CARE FACILITY; HEALTH SURVEY; LABORATORY AUTOMATION; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; REVIEW;

DNA PRIMERS; OLIGONUCLEOTIDES; SEQUENCE ANALYSIS, DNA;

EID: 0029745039     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (21)

1. Andrus, A. 1992. Oligonucleotide analysis by gel capillary electrophoresis. Methods: A Companion to Methods in Enzymology 4:213-226.
2. Brown, T. and D.J.S. Brown. 1991. Oligonucleotides and Analogues. A Practical Approach, p. 1-24. In F. Eckstein (Ed.). IRL Press, Oxford.
3. Clark, S. Multiple ALIGNment Editor (MALIGNED) [computer program]. Version 1.64. Division of Cellular and Molecular Biology, Ontario Cancer Institute, Toronto. A multiple sequence alignment program.
4. Demorest, D. and R. Dubrow. 1991. Factors influencing the resolution and quantitation of oligonucleotides separated by capillary electrophoresis on a gel-filled capillary. J. Chromatogr. 559:43-56.
5. Földes-Papp, Z., E. Birch-Hirschfeld, R. Rsch, M. Hartmann, A.K. Kleinschmidt and H. Seliger. 1995. Efficiency of chemical oligonucleotide synthesis evaluated by ion-exchange high performance liquid chromatography. J. Chromatogr. A. 706:405-419.
6. Gentil, E. and J. Banoub. 1996. Characterization and differentiation of isomeric self-complementary DNA oligomers by electrospray tandem mass spectrometry. J. Mass Spectrometry 31:83-94.
7. Heller, C. 1995. Capillary electrophoresis of proteins and nucleic acids in gels and entangled polymer solutions. J. Chromatogr. A. 698:19-31.
8. Ivanetich, K., R.L. Niece, M. Rohde, E. Fowler and T.K. Hayes. 1993. Biotechnology core facilities: trends and update. FASEB J. 7:1109-1114.2.
9. Karger, B.L., Y.H. Chu and F. Foret. 1995. Capillary electrophoresis of proteins and nucleic acids. Annu. Rev. Biophys. Biomol. Struct. 24:579-610.
10. Kaufman, J., M. Le, G. Ross, P. Hing, M. Budiansky, E. Yu, E. Campbell, V. Yoshimura, V. Fitzpatrick, K. Nadimi and A. Andrus. 1993. Trityl monitoring of automated DNA synthesizer operation by conductivity: a new method of real-time analysis. BioTechniques 14:834-839.
11. Little, D.P., R.A. Chorush, J.P. Speir, M.W. Senko, N.L. Kelleher and F.W. McLafferty. 1994. Rapid sequencing of oligonucleotides by high-resolution mass spectrometry. J. Am. Chem. Soc. 116:4893-4897.
12. Little, D.P., T.W. Thannhauser and F.W. McLafferty. 1995. Verification of 50- to 100-mer DNA and RNA sequences with high-resolution mass spectrometry. Proc. Natl. Acad. Sci. USA 92:2318-2322.
13. Mandecki, W., M.A. Shallcross and T.J. Kavanaugh. 1990. Simple biological assay for the error rate upon cloning of synthetic oligodeoxyribonucleotides. BioTechniques 9:56-58.
14. Naeve, C.W., G.A. Buck, R.L. Niece, R.T. Pon, M. Robertson and A.J. Smith. 1995. Accuracy of automated DNA sequencing: a multi-laboratory comparison of sequencing results. BioTechniques 19:448-453.
15. Perkin-Elmer/Applied Biosystems Division. 1992. Evaluating and isolating synthetic oligonucleotides. The complete guide, Publication No. 239902. Foster City, CA.
16. Pon, R.T. 1993. Methods in Molecular Biology, Vol 20, p. 465-496. In S. Agrawal (Ed.), Protocols for Oligonucleotides and Analogs. Humana Press, Totowa.
17. Pon, R.T., G.A. Buck, R.L. Niece, M. Robertson, A.J. Smith and E. Spicer. 1994. A survey of nucleic acid services in core laboratories. BioTechniques 17:526-534.
18. Reddy, M.P., N.B. Hanna and F. Farooqui. 1994. Fast cleavage and deprotection of oligonucleotides. Tetrahedron Lett. 35:4311-4314.
19. Srivatsa, G.S., M. Batt, J. Schuette, R.H. Carlson, J. Fitchett, C. Lee and D.L. Cole. 1994. Quantitative capillary gel electrophoresis assay of phosphorothioate oligonucleotides in pharmaceutical formulations. J. Chromatogr. A. 680:469-477.
20. Temsamani, J., M. Kubert and S. Agrawal. 1995. Sequence identity of the n-1 product of a synthetic oligonucleotide. Nucleic Acids Res. 25:1841-1844.
21. Wu, K.J., T.A. Shaler and C.H. Becker. 1994. Time-of-flight mass spectrometry of underivatized single-stranded DNA oligomers by matrix-assisted laser desorption. Anal. Chem. 66:1637-1645.