Interaction of human immunodeficiency virus type 1 reverse transcriptase with primer tRNA(Lys3) and affinity modification of the enzyme by tRNA(Lys3) derivatives

European Journal of Biochemistry

Volumn 240, Issue 3, 1996, Pages 774-780

  Nevinsky, Georgy A ^a   Zakharova, Ol'ga D ^a   Fournier, Michel ^b   Andreola, Marie Line ^b   Litvak, Simon ^b   Tarrago Litvak, Laura ^b  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

^b Institut de Biochimie et Genetique Cellulaires   (France)

Author keywords

Human immunodeficiency virus type 1 reverse transcriptase; K(d) value; tRNA(Lys3); tRNA(Lys3) affinity reagent

Indexed keywords

LYSINE TRANSFER RNA; RNA DIRECTED DNA POLYMERASE;

ARTICLE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; HUMAN IMMUNODEFICIENCY VIRUS; IN VITRO STUDY; PRIORITY JOURNAL; VIRUS REPLICATION;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0029743590     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0774h.x     Document Type: Article

References (25)

1. Lys with the p66/p66 form of HIV-1 reverse transcriptase stimulates DNA polymerase and ribonuclease H activities, J. Biol. Chem. 267, 19 356-19 362.
2. Barat, C., Lullien V., Schatz, O., Keith, G., Nugeyre, M. T., Grüninger-Leitch, F., Barré-Sinoussi, F., Le Grice, S. F. J. & Darlix, J. L. (1989) HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA, EMBO J. 8, 3279-3285.
3. Lys3, Nucleic Acids Res. 19, 751-757.
4. Barré-Sinoussi, F., Chermann, J. C., Rey, F., Nugeyre, M. T., Chamaret, S., Gruest, J., Dauguet, C., Axler-Blin, C., Vézinet-Brun, F., Rouzioux, C., Rozenbaum, W. & Montagnier, L. (1983) Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS), Science 220, 868-871.
5. Lys, Nucleic Acids Res. 18, 429-436.
6. Cornish-Bowden, A. (1976) in Principles of enzyme kinetics, pp. 27-28, 189-192, Butterworth, London and Boston.
7. Dixon, M. (1953) The determination of enzyme inhibitor constants, Biochem. J. 55, 170-171.
8. Trp. Mapping of complexed tRNA with chemicals and nucleases, Nucleic Acids Res. 12, 2259-2271.
9. Kitz, R. & Wilson, I. (1962) Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase, J. Biol. Chem. 237, 3245-3249.
10. Kolocheva, T. I., Nevinsky, G. A., Levina, A. S., Khomov, V. V. & Lavrik, O. I. (1991) The mechanism of recognition of templates by DNA polymerases from pro- and eucaryotes as revealed by affinity modification data, J. Biomol. Struct. Dyn. 9, 169-185.
11. Lys3: role of reverse transcriptase, Trends Biochem. Sci. 19, 114-118.
12. Litvak, S. (1996) Retroviral reverse transcriptases, R. G. Landes Publishing Co. Austin TX. Springer-Verlag, Heidelberg.
13. Lys3 to reverse transcriptase from HIV-1, EMBO J. 14, 2679-2687.
14. Nevinsky, G. A., Veniaminova, A. G., Levina, A. S., Podust, V. N., Lavrik, O. I. & Holler, E. (1990) Structure-function analysis of mononucleotides and short olgonucleotides in the priming of enzymatic DNA synthesis, Biochemistry 29, 1200-1207.
15. Oshevsky, S. I. (1982) Enzymatic transfer of ATPγS thiophosphate onto the 5′-hydroxyl of an oligonucleotide as a route to reactive oligonucleotide derivatives, FEBS Lett. 143, 119-123.
16. Popovic, M., Sarngadharan, M. G., Read, K. & Gallo, R. C. (1984) Detection, isolation and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS, Science 224, 497-500.
17. Lys induce important structural changes in HIV revese transcriptase, FEBS Lett. 277, 239-242.
18. Lys, Eur. J. Biochem. 184, 367-374.
19. Lys as evidenced by foot-printing studies and inhibition with synthetic oligoribonucleotides, J. Mol. Biol. 226, 1-6.
20. Skalka, A. M. & Goff, S. P. (eds) (1993) Reverse transcriptase, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY.
21. Tarrago-Litvak, L., Andreola, M. L., Nevinsky, G. A., Sarih-Cottin, L. & Litvak, S. (1994) The reverse transcriptase of HIV-1: from enzymology to therapeutic intervention, FASEB J. 8, 497-503.
22. Wain-Hobson, S., Sonigo, P., Danos, O. & Alizon, M. (1985) Nucleotide sequence of the AIDS virus LAV, Cell 40, 9-17.
23. Lys3 complexes, J. Biol. Chem. 268, 13 617-13 624.
24. Lys3 with the p51 subunit of HIV-1 reverse transcriptase: a possible role in enzyme activation, FEBS Lett. 361, 287-290.
25. Zakharova, O. D., Tarrago-Litvak, L., Maksakova, G., Andreola, M. L., Dufour, E., Litvak, S. & Nevinsky, G. A. (1995b) High-affinity interaction of human immunodeficiency virus type-1 reverse transcriptase with partially complementary primers, Eur. J. Biochem. 233, 856-863.