Analysis by flow cytometry of surface-exposed epitopes of outer membrane protein F of Pseudomonas aeruginosa

Canadian Journal of Microbiology

Volumn 42, Issue 8, 1996, Pages 859-862

  Hughes, Eileen E ^a   Matthews Greer, Janice M ^a   Gilleland Jr , H E ^a  

^a LOUISIANA STATE UNIV MEDICAL CENTER   (United States)

Author keywords

Flow cytometry; Outer membrane protein F.; Pseudomonas aeruginosa; Surface exposed epitopes

Indexed keywords

EPITOPE; OUTER MEMBRANE PROTEIN;

BACTERIAL OUTER MEMBRANE; CELL CYCLE; CONTROLLED STUDY; FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SHORT SURVEY;

EID: 0029742846     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/m96-109     Document Type: Short Survey

References (11)

1. Bowden, R.A., Cloeckaert, A., Zygmunt, M.S., Bernard, S., and Dubray, G. 1995. Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. Infect. Immun. 63: 3945-3952.
2. Gilleland, L.B., and Gilleland, H.E., Jr. 1995. Synthetic peptides representing two protective, linear B-cell epitopes of outer membrane protein F of Pseudomonas aeruginosa elicit whole-cell-reactive antibodies that are functionally pseudomonad specific. Infect. Immun. 63: 2347-2351.
3. Gilleland, H.E., Jr., Parker, M.G., Matthews, J.M., and Berg, R.D. 1984. Use of a purified outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine in mice. Infect. Immun. 44: 49-54.
4. Gilleland, H.E., Jr., Gilleland, L.B., and Matthews-Greer, J.M. 1988. Outer membrane protein F preparation of Pseudomonas aeruginosa as a vaccine against chronic pulmonary infection with heterologous immunotype strains in a rat model. Infect. Immun. 56: 1017-1022.
5. Gilleland, H.E., Jr., Hughes, E.E., Gilleland, L.B., Matthews-Greer, J.M., and Staczek, J. 1995. Use of synthetic peptides to identify surface-exposed, linear B-cell epitopes within outer membrane protein F of Pseudomonas aeruginosa. Curr. Microbiol. 31: 279-286.
6. Hughes, E.E., and Gilleland, H.E., Jr. 1995. Ability of synthetic peptides representing epitopes of outer membrane protein F of Pseudomonas aeruginosa to afford protection against P. aeruginosa infection in a murine acute pneumonia model. Vaccine, 13: 1750-1753.
7. Hughes, E.E., Gilleland, L.B., and Gilleland, H.E., Jr. 1992. Synthetic peptides representing epitopes of outer membrane protein F of Pseudomonas aeruginosa that elicit antibodies reactive with whole cells of heterologous immunotype strains of P. aeruginosa. Infect. Immun. 60: 3497-3503.
8. Kermani, P., Peloquin, L., and Lagace, J. 1945. Production of ScFv antibody fragments following immunization with a phage-displayed fusion protein and analysis of reactivity to surface-exposed epitopes of the protein F of Pseudomonas aeruginosa by cytofluorometry. Hybridoma, 14: 323-328.
9. Matthews-Greer, J.M., and Gilleland, H.E., Jr. 1987. Outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine against heterologous immunotype strains in a burned mouse model. J. Infect. Dis. 155: 1282-1291.
10. Matthews-Greer, J.M., Robertson, D.E., Gilleland, L.B., and Gilleland, H.E., Jr. 1990. Pseudomonas aeruginosa outer membrane protein F produced in Escherichia coli retains vaccine efficacy. Curr. Microbiol. 20: 171-175.
11. Nelson, D., Neill, W., and Poxton, I.R. 1990. A comparison of immunoblotting, flow cytometry and ELISA to monitor the binding of anti-lipopolysaccharide monoclonal antibodies. J. Immunol. Methods, 133: 227-233.