6-Phosphogluconate dehydrogenase from Trypanosoma brucei. Kinetic analysis and inhibition by trypanocidal drugs

European Journal of Biochemistry

Volumn 240, Issue 3, 1996, Pages 592-599

  Hanau, Stefania ^a   Rippa, Mario ^a   Bertelli, Massimo ^a   Dallocchio, Franco ^a   Barrett, Michael P ^b  

^a UNIVERSITY OF FERRARA   (Italy)

^b LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

6 Phosphogluconate dehydrogenase; Arsenicals; Pentose phosphate pathway; Suramin; Trypanosoma brucei

Indexed keywords

PHOSPHOGLUCONATE DEHYDROGENASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; LAMB; LIVER; NONHUMAN; PRIORITY JOURNAL; TRYPANOSOMA BRUCEI;

ANIMALIA; MAMMALIA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0029742699     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0592h.x     Document Type: Article

References (47)

1. Fairlamb, A. H. (1989) Novel biochemical pathways in parasitic protozoa, Parasitology 99, S93-S112.
2. Opperdoes, E. R. (1987) Compartmentation of carbohydrate metabolism in trypanosomes, Annu Rev. Microbiol. 41, 127-151.
3. Ryley, J. F. (1962) Studies on the metabolism of the protozoa, Biochem. J. 85, 211-223.
4. Bowman, I. B. R. (1974) In Trypanosomiasis and Leishmaniasis with special reference to Chagas' disease, Ciba Found. Symp. 20, 255-271.
5. Cronin, C. N., Nolan, D. P. & Voorheis, H. P. (1989) The enzymes of the classical pentose phosphate pathway are expressed differentially in procyclic and bloodstream forms of Trypanosoma brucei, FEBS Lett. 244, 26-30.
6. Fairlamb, A. H. & Cerami, A. (1992) Metabolism and functions of trypanothione in the kinetoplastida, Annu. Rev. Microbiol. 46, 695-729.
7. Lobo, Z. & Maitra, P. K. (1982) Pentose phosphate pathway mutants of yeast, Mol. Gen. Genet. 185, 367-368.
8. Gvodzdev, V. A., Gerasimova, T. I., Kogan, G. L. & Braslavskaya, O. Y. (1976) Role of the pentose phosphate pathway in metabolism of Drosophila melanogaster elucidated by mutations affecting glucose-6-phosphate and 6-phosphogluconate dehydrogenases, FEBS Lett. 64, 85-88.
9. Rosemeyer, M. A. (1987) The biochemistry of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and glutathione reductase, Cell Biochem. Funct. 5, 79-95.
10. Marchand, M., Kooystra, U., Wierenga, R. K., Lambeir, A.-M., Beeumen, J. V., Opperdoes, F. R. & Michels, P. A. M. (1989) Glucosephosphate isomerase from Trypanosoma brucei. Cloning and characterization of the gene and analysis of the enzyme, Eur. J. Biochem. 184, 455-464.
11. Barrett, M. P. & Le Page, R. W. F. (1993) A 6-phosphogluconate dehydrogenase gene from Trypanosoma brucei, Mol. Biochem. Parasitol. 57, 89-100.
12. Barrett, M. P., Phillips, C., Adams, M. A. & Le Page, R. W. F. (1994) Overexpression in Escherichia coli and purification of the 6-phosphogluconate dehydrogenase of Trypanosoma brucei, Protein Expr. Purif. 5, 44-49.
13. Phillips, C., Barrett, M. P., Gover, S., Le Page, R. W. F. & Adams, M. J. (1993) Preliminary crystallographic study of 6-phosphogluconate dehydrogenase from Trypanosoma brucei, J. Mol. Biol. 233, 317-321.
14. Horecker, B. L. (1955) Preparation and analysis of ribulose-5-phosphate, Methods Enzymol. 3, 190-192.
15. Hanau, S., Dallocchio, F. & Rippa, M. (1991) Identification of the lysine residue involved in the inactivation of lamb liver 6-phosphogluconate dehydrogenase by fluorescein 5′-isothiocyanate, Biochem. Int. 25, 613-620.
16. Lanham, S. M. & Godfrey, D. G. (1970) Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose, Exp. Parasitol. 28, 521-531.
17. Hirumi, H. & Hirumi, K. (1989) Continuous cultivation of Trypanosoma brucei bloodstream forms in a medium containing a low concentration of serum protein without a feeder cell layer, J. Parasitol. 75, 985-989.
18. Kaminsky, R. & Zweygarth, K. (1989) Feeder layer free in vitro assay for screening anti-trypanosomal compounds versus Trypanosoma brucei and Trypanosoma evansi, Antimicrob. Agents Chemother. 33, 881-885.
19. Carter, N. S. & Fairlamb, A. H. (1993) Arsenical-resistant trypanosomes lack an unusual adenosine transporter, Nature 361, 173-175.
20. Dixon, M. & Webb, E. C. (1964) Enzymes, 2nd edn., p. 116, Longmans Green, London.
21. Cleland, W. W. (1977) Adv. Enzymol. 45, 273-387.
22. Tipton, K. F. & Dixon, H. B. F. (1979) Methods Enzymol. 63, 183-234.
23. Medina-Puerta, M., Gallego-Iniesta, M. & Garrido-Pertierra, A. (1988) Kinetic analysis of 6-phosphogluconate dehydrogenase from bass liver: effects of temperature and pH on its catalytic function, Arch. Biochem. Biophys. 130, 130-141.
24. Dyson, J. D., D'Orazio, R. E. & Hanson, W. H. (1973) Sheep liver 6-phosphogluconate dehydrogenase: isolation procedure and effect of pH, ionic strength and metal ions on the kinetic parameters, Arch. Biochem. Biophys. 154, 623-635.
25. Rippa, M. & Pontremoli, S. (1968) Evidence of a critical histidine residue in 6-phosphogluconate dehydrogenase from Candida utilis, Biochemistry 7, 1514-1518.
26. Berdis, A. J. & Cook, P. F. (1993) Chemical mechanism of 6-phosphogluconate from Candida utilis from pH studies, Biochemistry 32, 2041-2046.
27. Rippa, M., Signorini, M. & Dallocchio, F. (1973) A multiple role for the coenzyme in the mechanism of action of 6-phosphogluconate dehydrogenase, J. Biol. Chem. 248, 4920-4925.
28. Hanau, S., Dallocchio, F. & Rippa, M. (1992) NADPH activates a decarboxylation reaction catalyzed by lamb liver 6-phosphogluconate dehydrogenase, Biochem. Biophys. Acta 1122, 273-277.
29. Dawson, R. M. Elliott, D. C., Elliott, W. H. & Jones, K. M. (1979) Data for biochemical research, 2nd edn, Claredon Press, Oxford.
30. Rippa, M. & Pontremoli, S. (1969) Pyridoxal 5′-phosphate as a specific photosensitizer for a histidine residue at the active site of 6-phosphogluconate dehydrogenase, Arch. Biochem. Biophys. 133, 112-118.
31. Rippa, M., Picco, C. & Pontremoli, S. (1970) Rose bengal as a specific sensitizer for a histidine residue at the triphosphopyridine nucleotide binding site of 6-phosphogluconate dehydrogenase, J. Biol. Chem. 245, 4977-4981.
32. Rippa, M., Picco, C., Signorini, M. & Pontremoli, S. (1971) Evidence for a tyrosine residue at the triphosphopyridinenucleotide-binding site of 6-phosphogluconate dehydrogenase, Arch. Biochem. Biophys. 147, 487-492.
33. Rippa, M., Signorini, M. & Pontremoli, S. (1972) Evidence for the involvement of a histidine residue in the binding of substrate to the 6-phosphogluconate dehydrogenase, Arch. Biochem. Biophys. 150, 503-510.
34. Procsal, D. & Holten, D. (1972) Purification and properties of 6-phosphogluconate dehydrogenase: activity at normal in vivo concentration of coenzyme, Biochemistry 11, 1310-1314.
35. Topham, C. M. & Dalziel, K. (1986) Chemical modification of sheep liver 6-phosphogluconate dehydrogenase by diethylpyrocarbonate, Eur. J. Biochem. 155, 87-94.
36. Topham, C. M., Matthews, B. & Dalziel, K. (1986) Kinetic studies of 6-phosphogluconate dehydrogenase from sheep liver, Eur. J. Biochem, 156, 555-567.
37. Adams, M. J., Ellis, G. H., Gover, S., Naylor, C. N. & Phillips, C. (1994) Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: implications for NADP specificity and the enzyme mechanism, Structure 2, 651-668.
38. Rippa, M., Spanio, L. & Pontremoli, S. (1967) A specific interaction of pyridoxal 5′-phosphate and 6-phosphogluconate dehydrogenase. Arch. Biochem. Biophys. 118, 48-57.
39. Dallocchio, F., Negrini, R., Signorini, M. & Rippa, M. (1976) Identification of the chemical groups involved in the binding of periodate oxidised NADP to 6-phosphogluconate dehydrogenase, Biochim. Biophys. Acta 429, 629-634.
40. Hanau, S., Dallocchio, F. & Rippa, M. (1992) Subunit asymmetry in the ternary complex of lamb liver 6-phosphogluconate dehydrogenase detected by a NADP analogue. Biochim. Biophys. Acta 1159, 262-266.
41. Hanau, S., Dallocchio, F. & Rippa, M. (1992) Use of trinitrobenzensulfonate for affinity labelling of lysine residue at the phosphate binding sites of some enzyme, Arch. Biochem. Biophys. 302, 218-221.
42. Fairlamb, A. H. & Bowman, I. B. R. (1980) Uptake of the trypanocidal drug suramin by bloodstream forms of Trypanosoma brucei and its effect on respiration and growth rate in vivo, Mol. Biochem. Parasitol. 1, 315-333.
43. Perie, J., Riviere-Arlac, I., Blonski, C., Geflaut, T., Lauth de Viguerie, N., Trinquier, M., Willson, M., Opperdoes, F. R. & Callens, M. (1993) Inhibition of the glycolytic enzymes in the trypanosome: an approach in the development of new leads in the therapy of parasitic diseases, Pharmac. Ther. 60, 347-365.
44. Voogd, T. E., Vansterkenburg, E. L. M., Wilting, J. & Janssen, L. H. M. (1993) Recent research on the biological activity of suramin, Pharmacol. Rev. 45, 177-203.
45. Rippa, M. Signorini, M., Bellini, T. & Dallocchio, F. (1978) Evidence for the proximity of two sulphydryl groups at the active site of 6-phosphogluconate dehydrogenase, Arch. Biochem. Biophys. 186, 406-410.
46. Rippa, M., Bellini, T., Signorini, M. & Dallocchio, F. (1981) Evidence for multiple pairs of vicinal thiols in some proteins, J. Biol. Chem. 256, 451-455.
47. Cunningham, M. L., Zvelebil, M. J. J. M. & Fairlamb A. H. (1994) Mechanism of inhibition of trypanothione reductase and glutathione reductase by trivalent organic arsenicals, Eur. J. Biochem. 221, 285-295.