Significance of ammonia in the genesis of gastric epithelial lesions induced by helicobacter pylori: An in vitro study with different bacterial strains and urea concentrations

Digestion

Volumn 57, Issue 5, 1996, Pages 299-304

  Sommi, P ^a,b   Ricci, V ^a   Fiocca, R ^b   Romano, M ^c   Ivey, K J ^d   Coma, E ^a   Solcia, E ^b   Ventura, U ^a  

^a Institute of Human Physiology   (Italy)

^b FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^d VA LONG BEACH HEALTHCARE SYSTEM   (United States)

Author keywords

Acetohvdroxamic acid; Ammonia; Cell vacuolation; Cultured gastric cells; Helicobacter pylori; Urea; Urease; Vac A

Indexed keywords

ACETOHYDROXAMIC ACID; AMMONIA; BACTERIAL TOXIN; UREA; UREASE; UREASE INHIBITOR;

ARTICLE; BACTERIUM CULTURE; CELL DAMAGE; CELL VACUOLE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; STOMACH DISEASE; STOMACH EPITHELIUM; STOMACH MUCOSA;

AMMONIA; BACTERIAL PROTEINS; BACTERIAL TOXINS; GASTRIC MUCOSA; HELICOBACTER PYLORI; HUMANS; HYDROXAMIC ACIDS; UREA; UREASE; VACUOLES;

EID: 0029740061     PISSN: 00122823     EISSN: 14219867     Source Type: Journal    
DOI: 10.1159/000201349     Document Type: Article

References (44)

1. Warren JR, Marshall BJ: Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1983;i: 1273-1275.
2. Goodwin CS, Amstrong JA, Marshall BJ: Campylobacter pyloridis. gastritis and peptic ulceration. J Clin Pathol 1986:39:353-365.
3. Bode G, Malfertheiner P, Ditschuneit H: Pathogenetic implications of ultrastructural findings in Campylobacter pylori related gastroduodenal disease. Scand J Gastroenterol 1988;23(suppl 142):25-39.
4. Fiocca R, Viliam L, Luinetti O, Gianatli A, Perego M, Alvisi C, Turpini F, Solcia F: Helicobacter colonization and histopathological profile of chronic gastritis in patients with or without dyspepsia, mucosal erosion and peptic ulcer: A morphological approach to the study of uleerogcncsis in man. Virchows Arch (A) 1992: 420:489-498.
5. NIH Consensus Development Panel: Helicobacter pylori in peptic ulcer disease. J Am Med Assoc 1994;272:65-69.
6. World Health Organization: Schistosomes, liver flukes and Helicobacter pylori. 1ARC Monogr Eval Carcinog Risks Hum 1994:61: 177-240.
7. Graham DY: Campylobacter pylori and peptic ulcer disease. Gastroenterology 1989:96:615-625.
8. Marshall BJ, Barrel1 U, Prakash C, McCallum RW, Guerrant RL: Urea protects Helicobacter (Campylobacter) pylori from the bactericidal effect of acid. Gastroenterology 1990:99:697-702.
9. Megraud F, Lamouliatte II: Helicobacter pylori and duodenal ulcer. Evidence suggesting causation. DigDisSci 1992:37:769-772.
10. Tsujii M, Kawano S, Tsuji S, Nagalo K, Ito T, Hayashi N, Fusamoto H, Kamata I, Tamura K: Ammonia, a possible promoter in Helicobacter pylori-related gastric carcinogenesis. Cancer Lett 1992:65:15-18.
11. Tsujii M, Kawano S, Tsuji S, Fusamoto H, Kamada T, Sato N: Mechanism of gastric mucosal damage induced by ammonia. Gastroenterology 1992:102:1881-1888.
12. Triebling AT, Kosten MA, Dlugosz JW, Paro-netto F, Lieber CS: Severity of Helicobacter-induced gastric injury correlates with gastric juice ammonia. Dig Dis Sci 1991:36:1089-1096.
13. Cover TL, Blaser MJ: Purification and characterization of the vacuolating toxin from Helicobacter pylori. J Biol Chem 1992:267:10570-10575.
14. Cover TL, Cao P, Lind CD, Tham KT, Blaser MJ: Correlation between vacuolatingcytotoxin production by Helicobacter pylori isolates in vitro and in vivo. Infect Immun 1993:61: 5008-5012.
15. Tummuru MKR, Cover TL, Blaser MJ: Cloning and expression of a high-molccular-mass major antigen of Helicobacter pylori Evidence of linkage to cytotoxin production. Infect Immun 1993:61:1799-1809.
16. Covacci A, Censini S, Bugnoli M, Petracca R, Burroni D, Macchia G, Massonc A, Papini E. Xiang Z. Figura N. Rappuoli R: Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci USA 1993:90:5791-5795.
17. Xiang Z, Censini S, Baveli PF, Telford JL, Figura N, Rappuoli R, Covacci A: Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect Immun 1995:63:94-98.
18. Telford JL, Covacci A, Ghiara P, Montecucco C, Rappuoli R: Unravelling the pathogenic role of Helicobacter pylori in peptic ulcer: Potential new therapies and vaccines. Trends Biotechnol 1994:12:420-426.
19. Sommi P, Rieci V, Fiocca R, Cova E, Figura N, Romano M, Ivey KJ, Solcia E, Ventura U: Biochemical evaluation of cytotoxic effects of Helicobacter pylori(MP)on MKN 28 human gastric epithelial cell line. Ir J Med Sei 1992:161 (suppl 10):9.
20. Ricci V, Sommi P, Cova E, Fiocca R, Figura N, Romano M, Ivey KJ, Solcia E, Ventura U: Vacuolating and cytolcthal effects of Helicobacter pylori broth culture filtrate on MKN 28 human gastric epithelial cell line. Gastroenterology 1992; 102:A684.
21. Ricci V, Sommi P, Fiocca R, Cova E, Figura N, Romano M, Ivey KJ, Solcia E, Ventura U: Cytotoxicity of Helicobacter pylori on human gastric epithelial cells in vitro: Role of cvtotox-in(s) and ammonia. Eur J Gastroenterol Hepatol 1993;5:687-694.
22. Marchetti M, Aricö B, Burroni D, Figura N, Rappuoli R, Ghiara P: Development of a mouse model of Helicobacter pylori infection that mimics human disease. Science 1995:267: 1655-1658.
23. Crabtree JE, Wyatt JL, Sobala GM, Miller G, Tompkins DS, Primrose JN, Morgan AG: Systemic and mucosal humoral responses to Helicobacter pylori in gastric cancer. Gut 1993:34: 1339-1343.
24. Neman-Simha V, Briigmann D, Megraud F: Interaction of Campylobacter pylori with epithelial cells: Role of the urease. Klin Wo-chensehr 1989;67(suppl XVIII):48.
25. Megraud F, Neman-Simha V, Briigmann D: Further evidence of the toxic effect of ammonia produced by Helicobacter pylori urease on human epithelial cells. Infect Immun 1992:60: 1858-1863.
26. liojo H: Establishment of cultured lines of human stomach cancer. Origin and their morphological characteristics. Niigata Igakukai Zasshi 1977;91:737-752.
27. Romano M, Razandi M, Sekhon S, Krause WJ, Ivey KJ: Human cell line for study of damage to gastric epithelial cell in vitro. J Lab Clin Med 1988:111:430-440.
28. Rieci V, Fiocca R, Sommi P, Cova E, Romano M, Luinetti O, Barattini P, Ivey KJ, Solcia E, Ventura U: MKN 28 cell line: A useful tool to study human gastric epithelial cells. Pflügers Arch 1992;420:RI82.
29. Cover TL, Puryear W, Percz-Percz GI, Blaser MJ: Effects of urease on HcLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infect Immun 1991;59:1264-1270.
30. Smoot DT, Mobley HLT, Chippendale GR, Lewison JF, Resau JH: Helicobacter pylori urease activity is toxic to human gastric epithelial cells. Infect Immun 1990;58:1992-1994.
31. Tamawski A, Erickson RA: Sucralfate-24 years later: Current concepts of its protective and therapeutic action. Eur J Gastroenterol Hcpalol 1991:3:795-810.
32. Mondzac A, Ehrlich GE, Secgmiller JF.: An enzymatic determination of ammonia in biological fluids. J Lab Clin Med 1965:66:526-531.
33. Snedecor GW, Cockran WG: Statistical Methods. Ames. The Iowa State University Press. 1967.
34. Dick JD: Helicobacter (Campylobacter) pylori: A new twist to an old disease. Annu Rev Microbiol 1990:44:249-269.
35. Von Wulffcn H, Marquardt T: Cytotoxic activity of Helicobacter pylori enhanced by acetohy-droxamic acid. J Clin Pathol 1994:47:188-189.
36. Cover TL, Vaughn SG, Cao P, Blaser MJ: Potentiation of Helicobacter pylori vacuolating toxin activity by nicotine and other weak bases. J Infect Dis 1992:166:1073-1078.
37. Xu JK, Goodwin CS, Cooper M, Robinson J: Intracellular vacuolization caused by the urease of Helicobacter pylori. J Infect Dis 1990; 161:1302-1304.
38. Hazell SL, Borody TJ, Gal A, Lee A: Campylobacter pyloridis gastritis. Am J Gastroenterol 1987,82:292-296.
39. Hazell SL, Lee A: Campylobacter pyloridis. urease, hydrogen ion back diffusion, and gastric ulcers. Lancet 1986;ii: 15-17.
40. Takahashi S, Igarashi H, Ishiyma N, Nakamura K, Ozaki M, Nakano M, Ito M, Saito S, Aoyagi T, Yamagishi T, Itoh T, Hirata I: Helicobacter pylori and ammonia in gastritis. Mi-crob Ecol Health Dis 1991;4:S143.
41. Tsujii M, Kawano S, Tsuji S, Fusamoto H, Kamada T, Sato N: Mechanism of gastric mucosal damage induced by ammonia. Gastroenterology 1992:102:1881-1888.
42. Murakami M, Yoo J, Sciki M, Inada M, Mitake T: Destruction of the gastric mucosal barrier by ammonia in rats and dogs. Gastroenterology 1988:94:A317.
43. Suzuki M, Miura S, Suematsu M, Fukumura D. Karose I, Suzuki H, Kai A, Kudoh Y, Oha-shi M, Tsuchiya M: Helicobacter pylori-associ-ated ammonia production enhances neutrophil-dependent gastric mucosal cell injury. Am J Physiol 1992:263:G719-G725.
44. Ricci V, Sommi P, Cova E, Marelli R, Fiocca R, Romano M, Ivey KJ, Solcia E, Ventura U: Helicobacter pylori-induced cell vacuolation: Cytotoxin potentiated the action of ammonia. Gastroenterology 1994:106:A165.