Cysteine concentrations in rodent tumors: Unexpectedly high values may cause therapy resistance

International Journal of Cancer

Volumn 67, Issue 5, 1996, Pages 661-667

  Koch, Cameron J ^a,b   Evans, Sydney M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b 195 John Morgan Bldg ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; GLUTATHIONE;

ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FEMALE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; RADIOSENSITIVITY; RAT; TUMOR CELL LINE;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; ESOPHAGEAL NEOPLASMS; FEMALE; GLIOMA; GLUTATHIONE; HUMANS; LIVER NEOPLASMS, EXPERIMENTAL; MALE; MAMMARY NEOPLASMS, EXPERIMENTAL; MICE; MICE, INBRED C3H; NEOPLASMS, EXPERIMENTAL; RATS; RATS, INBRED BUF; SARCOMA, EXPERIMENTAL; TREATMENT OUTCOME;

EID: 0029739455     PISSN: 00207136     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0215(19960904)67:5<661::AID-IJC12>3.0.CO;2-8     Document Type: Article

References (23)

1. ALLALUNIS-TURNER, M.J., LEE, F.Y.F. and SIEMANN, D.W., Comparison of glutathione levels in rodent and human tumor cells grown in vitro and in vivo. Cancer Res., 48, 3657-3660 (1988).
2. ALLISON, L.A. and SHOUP, R.E., Dual electrode liquid chromatography detector for thiols and disulfides. Anal. Chem., 55, 8-12 (1983).
3. BAKER, M.A., CERNIGLIA, G.J. and ZAMAN, A., Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal. Biochem., 190, 360-365 (1990).
4. BUMP, E.A., CERCE, B.A., AL-SARRAF, R., PIERCE, S.M. and KOCH, C.J., Radioprotection of DNA in isolated nuclei by naturally occurring thiols at intermediate oxygen tension. Rad. Res., 132, 94-104 (1992).
5. ELLMAN, G.L., Tissue sulfhydryl groups. Arch. Biochem. Biophys., 82, 70-77 (1959).
6. EVANS, S.M. and KOCH, C.J., Characterization of the 9L glioma as a tissue isolated epigastric implant. Rad. Oncol. Invest., 2, 134-143 (1994).
7. EVANS, S.M., KOCH, C.J., JOINER, B., JENKINS, W.T., LAUOHLIN, K.M. and LORD, E.M., Identification of hypoxia in cells and tissues of epigastric 9L rat glioma using EF5 [2-(2-nitro-1H-imidazol-l-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide]. Brit. J. Cancer, 72, 875-882 (1995).
8. FAHEY, R.C., DORIAN, R., NEWTON, G.L. and UTLEY, J., Determination of intracellular thiol levels using bromobimane fluorescent labeling: applications involving radioprotective drugs. In: O.F. Nygaard and M.G. Simic (eds.), Radioprotectors and anticarcinogens, pp. 103-120, Academic Press, New York (1983).
9. FAHEY, R.C. and SUNDQUIST, A.R., Evolution of glutathione metabolism. Adv. Enzymol., 64, 1-53 (1991).
10. JOCELYN, P.C., Biochemistry of the sulfhydryl group. Academic Press, New York (1972).
11. KOCH, C.J., Competition between radiation protectors and radiation sensitizers in mammalian cells. In: O.F. Nygaard and M.G. Simic (eds.), Radioprotectors and anticarcinogens, pp. 275-296, Academic Press, New York (1983).
12. KOCH, C.J. and SKOV, K.A., Comparisons of cellular radiation response using absolute rather than relative parameters. Rad. Res., 132, 40-49 (1992).
13. KOCH, C.J. and SKOV, K.A., Enhanced radiation sensitivity by preincubation with nitroimidazoles: effect of glutathione depletion. Int. J. Rad. Biol. Oncol. Phys., 29, 345-349 (1994).
14. LOH, S.N., DETHLEFSEN, L.A., NEWTON, G.L., AGULIERA, J.A. and FAHEY, R.C., Nuclear thiols: technical limitations on the determination of endogenous nuclear glutathione and the potential importance of sulfhydryl proteins. Rad. Res., 121, 98-106 (1990).
15. MEISTER, A., ANDERSON, M.E. and HWANG, O., Intracellular cysteine and glutathione delivery systems. J. Amer. Coll. Nutr., 5, 137-151 (1986).
16. O'DWYER, P.J., YAO, K.-S., FORD, P., GODWIN, A.K. and CLAYTON, M., Effects of hypoxia on detoxicating enzyme activity and expression in HT29 colon adenocarcinoma cells. Cancer Res., 54, 3082-3087 (1994).
17. POST, G.B., KELLER, D.A., CONNOR, K.A. and MENZEL, D.B., Effects of culture conditions on glutathione content in A549 cells. Biochem. biophys. Res. Comm., 114, 737-742 (1983).
18. REED, D.J., Glutathione; toxicological implications. Ann. Rev. Pharmacol. Toxicol., 30, 603-631 (1990).
19. ROBERTS, J.C. and FRANCETIC, D.J., The importance of sample preparation and storage in glutathione analysis. Anal. Biochem., 211, 183-187 (1993).
20. STANDEVEN, A.M. and WETTERHAHN, K.E., Tissue-specific changes in glutathione and cysteine after buthionine sulfoximine treatment of rats and the potential for artifacts in thiol levels resulting from tissue preparation. Toxicol. appl. Pharmacol., 107, 269-284 (1991).
21. TEIXEIRA, C.C., SHAPIRO, I.M., HATORI, M., RAJUROHIT, R. and KOCH, C.J., Retinoic acid modulation of glutathione and cysteine metabolism in chondrocytes. Biochemistry, (1996) (In press).
22. TIETZE, F., Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione. Anal. Biochem., 27, 502-522 (1969).
23. ZHENG, S., NEWTON, G.L., GONICK, G., FAHEY, R.C. and WARD, J.F., Radioprotectipn of DNA by thiols: relationship between the net charge of a thiol and its ability to protect DNA. Rad. Res., 114, 11-27 (1988).