Fluorescent labelling of nascent RNA reveals nuclear transcription domains throughout plant cell nuclei

Protoplasma

Volumn 192, Issue 3-4, 1996, Pages 145-149

  Straatman, K R ^a   Trompetter, C M ^a   Schul, W ^b   Schel, J H N ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

5 Bromouridine 5' triphosphate incorporation; Brassica napus; Confocal scanning laser microscopy; Immunolabelling; Isolated nuclei; Transcription sites

Indexed keywords

BRASSICA NAPUS; MAMMALIA; SINAPIS ARVENSIS;

EID: 0029738641     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF01273886     Document Type: Article

References (21)

1. Beven AF, Simpson GG, Brown JWS, Shaw PJ (1995) The organization of spliceosomal components in the nuclei of higher plants. J Cell Sci 108: 509-518
2. Binarova P, Straatman K, Hause B, Hause G, Van Lammeren AAM (1993) Nuclear DNA synthesis during the induction of embryogenesis in cultured microspores and pollen of Brassica napua L. Theor Appl Genet 87: 9-16
3. Dirks RW, Daniel KC, Raap AK (1995) RNAs radiate from gene to cytoplasm as revealed by fluorescence in situ hybridization. J Cell Sci 108: 2565-2572
4. Garcia-Blanco MA, Miller DD, Sheetz MP (1995) Nuclear spreads: I. Visualization of bipartite ribosomal RNA domains. J Cell Biol 128: 15-27
5. Gustavsson H-O, Ellerström M, Stålberg K, Azcurra I. Koman A, Höglund A-S, Rask L, Josefsson L-G (1991) Distinct sequence elements in a napin promoter interact in vitro with DNA-binding proteins from Brassica napus. Physiol Plant 82: 205-212
6. Hause B, Hause G, Pechan P, Van Lammeren AAM (1993) Cytoskeletal changes and induction of embryogenesis in microspore and pollen cultures of Brassica napus L. Cell Biol Int 17: 153-168
7. Hause G, Cordewener JHG, Ehrmanova M, Hause B, Binarova P, Van Lookeren Campagne MM, Van Lammeren AAM (1995) Cell cycle dependent distribution of phosphorylated proteins in microspores and pollen of Brassica napus L., detected by the monoclonal antibody MPM-2. Protoplasma 187: 117-126
8. Hozák P, Cook PR, Schöfer C, Mosgöller W, Wachtler F (1994) Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J Cell Sci 107: 639-648
9. Huang S, Spector DL (1991) Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors. Genes Dev 5: 2288-2302
10. Jackson DA, Hassan AB, Errington RJ, Cook PR (1993) Visualization of focal sites of transcription within human nuclei. EMBO J 12: 1059-1065
11. Lawrence JB, Singer RH, Marselle LM (1989) Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization. Cell 57: 493-502
12. Nakayama C, Saneyoshi M (1984) Utilizations of various uridine 5′-triphosphate analogues by DNA dependent RNA polymerases I and II purified from liver nuclei of cherry salmon (Oncorynchus mason). J Biochem 96: 1501-1509
13. Raap AK, van de Rijke FM, Dirks RW, Sol CJ, Boom R, van der Ploeg M (1991) Bicolor fluorescence in situ hybridization to intron and exon mRNA sequences. Exp Cell Res 197: 319-322
14. Risueño MC, Testillano PS (1994) Cytochemistry and immunocytochemistry of nucleolar chromatin in plants. Micron 25: 331-360
15. Roeder RG (1976) Eukaryotic nuclear RNA polymerases. In: Losick R, Chamberlin M (eds) RNA polymerases. Cold Spring Harbor, New York, pp 285-329
16. Sentenac A (1985) Eukaryotic RNA polymerases. CRC Crit Rev Biochem 18: 31-90
17. Sjödahl S, Gustavsson H-O, Rödin J, Lenman M, Höglund A-S, Rask L (1993) Cruciferin gene families are expressed coordinately but with tissue-specific differences during Brassica nupus seed development. Plant Mol Biol 23: 1165-1176
18. Spector DL (1993) Macromolecular domains within the cell nucleus. Annu Rev Cell Biol 9: 265-315
19. Stuurman N, de Graaf A, Floore A, Josse A, Humbel B. de Jong L, van Driel R (1992) A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies. J Cell Sci 101: 773-784
20. Testillano PS, Sánchez-Pina MA, Olmedilla A, Fuchs JP, Risueño MC (1993) Characterization of the interchromatin region as the nuclear domain containing snRNPs in plant cells. A cytochemical and immunoelectron microscopy study. Eur J Cell Biol 61: 349-361
21. Wansink DG, Schul W, van der Kraan I, van Steensel B, van Driel R, de Jong L (1993) Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus. J Cell Biol 122: 283-293