Kinetics of mRNA and protein synthesis of genes controlled by the 1,4- β-endoxylanase A promoter in controlled fermentations of Aspergillus awamori

Applied and Environmental Microbiology

Volumn 62, Issue 10, 1996, Pages 3646-3649

  Gouka, Robin J ^a,b   Stam, Hein ^b,c   Fellinger, Arthur J ^b   Muijsenberg, Rob J G T ^b   Van De Wijngaard, Arjan J ^a,b   Punt, Peter J ^a   Musters, Wouter ^b   Van Den Hondel, Cees A M J J ^a  

^a TNO   (Netherlands)

^b UNILEVER RESEARCH VLAARDINGEN   (Netherlands)

^c Givaudan SA   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

TRIACYLGLYCEROL LIPASE; XYLOSE;

ARTICLE; ASPERGILLUS AWAMORI; CELL CULTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE EXPRESSION; GENE EXPRESSION REGULATION; GLUCOSE METABOLISM; PROMOTER REGION; PROTEIN SYNTHESIS; RNA ANALYSIS;

ASPERGILLUS AWAMORI; ESCHERICHIA COLI; THERMOMYCES LANUGINOSUS;

EID: 0029738427     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.10.3646-3649.1996     Document Type: Article

References (15)

1. Broekhuijsen, M. P., I. E. Mattern, R. Contreras, J. R. Kinghorn, and C. A. M. J. J. van den Hondel. 1993. Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein. J. Biotechnol. 31:135-145.
2. Brown, A. J. P. 1989. Messenger RNA stability in yeast. Yeast 5:239-258.
3. Brown, A. J. P., I. J. Purvis, T. Chinnappan Santiago, A. J. E. Bettany, L. Loughlin, and J. Moore. 1988. Messenger RNA degradation in Saccharomyces cerevisiae. Gene 72:151-160.
4. Davies, R. W. 1994. Heterologous gene expression and protein secretion in Aspergillus, p. 527-560. In S. D. Martinelli, and J. R. Kinghorn (ed.), Aspergillus: 50 years on. Elsevier Science BV, Amsterdam.
5. Fowler, T., and R. M. Berka. 1991. Gene expression systems for filamentous fungi. Curr. Opin. Biotechnol. 2:691-697.
6. Gouka, R. J., J. G. M. Hessing, P. J. Punt, H. Stam, W. Musters, and C. A. M. J. J. van den Hondel. An expression system based on the promoter region of the 1,4-β-endoxylanase A gene of Aspergillus awamori. Appl. Microbiol. Biotechnol., in press.
7. Gouka, R. J., P. J. Punt, J. G. M. Hessing, and C. A. M. J. J. van den Hondel. 1996. Heterologous gene expression in Aspergillus awamori: comparison of expression levels of genes from different origins. Appl. Environ. Microbiol. 62:1951-1957.
8. Hessing, J. G. M., C. van Rotterdam, J. M. A. Verbakel, M. Roza, J. Maat, R. F. M. van Gorcom, and C. A. M. J. J. van den Hondel. 1994. Isolation and characterization of a 1,4-beta-endoxylanase gene of A. awamori. Curr. Genet. 26:228-232.
9. Kolar, M., P. J. Punt, C. A. M. J. J. van den Hondel, and H. Schwab. 1988. Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene. Gene 62:127-134.
10. Raué, H. A. 1994. Metabolic stability of mRNA in yeast - a potential target for modulating productivity. Trends Biotcchnol. 12:444-449.
11. Roberts, I. N., R. P. Oliver, P. J. Punt, and C. A. M. J. J. van den Hondel. 1989. Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Curr. Genet. 15:177-180.
12. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
13. Sierkstra, L. N., N. P. Nouwen, J. M. A. Verbakel, and C. T. Verrips. 1992. Analysis of glucose repression in Saccharomyces cerevisiae by pulsing glucose to a galactose-limited continuous culture. Yeast 8:1077-1087.
14. van den Hondel, C. A. M. J. J., P. J. Punt, and R. F. M. Van Gorcom. 1991. Heterologous gene expression in filamentous fungi, p. 396-428. In J. W. Bennet and L. Lasure (ed.), More gene manipulations in fungi. Academic Press, San Diego, Calif.
15. Verdoes, J. C., P. J. Punt, and C. A. M. J. J. van den Hondel. 1995. Molecular genetic strain improvement for the overproduction of fungal proteins by filamentous fungi. Appl. Microbiol. Biotechnol. 43:195-205.