Mutational analysis of the influenza virus A/Victoria/3/75 PA protein: Studies of interaction with PB1 protein and identification of a dominant negative mutant

Journal of General Virology

Volumn 77, Issue 8, 1996, Pages 1745-1749

  Zürcher, Thomas ^a,b   De La Luna, Susana ^a   Sanz Ezquerro, Juan J ^a   Nieto, Amelia ^a   Ortín, Juan ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MUTANT PROTEIN; RNA POLYMERASE; VIRUS ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; INFLUENZA VIRUS A; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; STRUCTURE ACTIVITY RELATION; TEMPERATURE SENSITIVE MUTANT; VIRUS MUTANT;

EID: 0029737653     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-77-8-1745     Document Type: Article

References (23)

1. Aso, T., Vasavada, H. A., Kawaguchi, T., Germino, F. J., Ganguly, S., Kitajima, S., Weissman, S. M. & Yasukochi, Y. (1992). Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF. Nature 355, 461-464.
2. Biswas, S. K. & Nayak, D. P. (1994). Mutational analysis of the conserved motifs of influenza A virus polymerase basic protein 1. Journal of Virology 68, 1819-1826.
3. Brasier, A. R., Tate, J. E. & Habener, J. F. (1989). Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. BioTechniques 7, 1116-1122.
4. de la Luna, S., Martín, J., Portela, A. & Ortín, J. (1993). Influenza virus naked RNA can be expressed upon transfection into cells co-expressing the three subunits of the polymerase and the nucleoprotein from simian virus 40 recombinant viruses. Journal of General Virology 74, 535-539.
5. Digard, P., Blok, V. C. & Inglis, S. C. (1989). Complex formation between influenza virus polymerase proteins expressed in Xenopus oocytes. Virology 171, 162-169.
6. Elroy-Stein, O., Fuerst, T. R. & Moss, B. (1989). Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5′ sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system. Proceedings of the National Academy of Sciences, USA 86, 6126-6130.
7. Fodor, E., Seong, B. L. & Brownlee, G. G. (1993). Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. Journal of General Virology 74, 1327-1333.
8. García-Sastre, A. & Palese, P. (1993). Genetic manipulation of negative-strand RNA virus genomes. Annual Review of Microbiology 47, 765-790.
9. Herget, M. & Scholtissek, C. (1993). A temperature-sensitive mutation in the acidic polymerase gene of an influenza A virus alters the regulation of viral protein synthesis. Journal of General Virology 74, 1789-1794.
10. Krug, R. M., Alonso-Kaplen, F. V., Julkunen, I. & Katze, M. G. (1989). Expression and replication of the influenza virus genome. In The Influenza Viruses, pp. 89-152. Edited by R. M. Krug. New York: Plenum Press.
11. Licheng, S., Summers, D. F., Peng, Q. & Galarza, J. M. (1995). Influenza A virus polymerase subunit PB2 is the endonuclease which cleaves host cell mRNA and functions only as the trimeric enzyme. Virology 208, 38-47.
12. Mahy, B. W. J. (1983). Mutants of influenza virus. In Genetics of Influenza Viruses, pp. 192-253. Edited by P. Palese and D. W. Kingsbury. Wien: Springer Verlag.
13. Mandler, J., Müller, K. & Scholtissek, C. (1991). Mutants and revertants of an avian influenza A virus with temperature-sensitive defects in the nucleoprotein and PB2. Virology 181, 512-519.
14. Mena, I., de la Luna, S., Albo, C., Martín, J., Nieto, A., Ortín, J. & Portela, A. (1994). Synthesis of biologically active influenza virus core proteins using a vaccinia virus-T7 RNA polymerase expression system. Journal of General Virology 75, 2109-2114.
15. Mena, I., de la Luna, S., Martín, J., Albó, C., Perales, B., Nieto, A., Portela, A. & Ortín, J. (1995). Systems to express recombinant RNA molecules by the influenza A virus polymerase in vivo. In Methods in Molecular Genetics. Molecular Virology Techniques, Part B, pp. 329-342. Edited by K. W. Adolph. Orlando: Academic Press.
16. Perales, B., de la Luna, S., Palacios, I. & Ortín, J. (1996). Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunit. Journal of Virology 70, 1678-1686.
17. Piccone, M. E., Fernandez, S. A. & Palese, P. (1993). Mutational analysis of the influenza virus vRNA promoter. Virus Research 28, 99-112.
18. Poch, O., Sauvaget, I., Delarue, M. & Tordo, N. (1990). Identification of four conserved motifs among the RNA-dependent polymerases encoding elements. EMBO Journal 8, 3867-3874.
19. Sadowski, I. & Patshne, M. (1989). A vector expressing GAL4 (1-147) fusion in mammalian cells. Nucleic Acids Research 17, 7539.
20. Sadowski, I., Ma, J., Triezenberg, S. & Pathsne, M. (1988). GAL4-VP16 is an unusually potent transcriptional activator. Nature 335, 563-564.
21. Sanz-Ezquerro, J. J., de la Luna, S., Ortín, J. & Nieto, A. (1995). Individual expression of influenza virus PA protein induces degradation of co-expressed proteins. Journal of Virology 69, 2420-2426.
22. Sanz-Ezquerro, J. J., Zürcher, T., de la Luna, S., Ortín, J. & Nieto, A. (1996). The amino-terminal one-third of the influenza virus PA protein is responsible for the induction of proteolysis. Journal of Virology 70, 1905-1911.
23. Ulmanen, I., Broni, B. A. & Krug, R. M. (1981). The role of two of the influenza virus core P proteins in recognizing cap I structures (m7GpppNm) on RNAs and in initiating viral RNA transcription. Proceedings of the National Academy of Sciences, USA 78, 7355-7359.