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Volumn 120, Issue 1, 1996, Pages 29-34

Systematic peptide fragmentation of polyvinylidene difluoride(PVDF)-immobilized proteins prior to microsequencing

Author keywords

Deducible amino acid residues; PVDF membrane; Recoveries of digests from PVDF; S alkylation of protein; Sequential in situ digestion

Indexed keywords

BACTERIAL ENZYME; POLYVINYLIDENE FLUORIDE; PROTEINASE;

EID: 0029737640     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021389     Document Type: Article
Times cited : (54)

References (10)
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    • Iwamatsu, A. (1992) S-Carboxymethylation of proteins transferred onto polyvinylidene difluoride membranes followed by in situ protease digestion and amino acid microsequencing. Electrophoresis 13, 142-147
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    • Iwamatsu, A.1
  • 2
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    • Microsequencing of proteins electrotransferred onto immobilizing matrices from polyaerylamide gel electrophoresis: Application to an insoluble protein
    • Hirano, H. and Watanabe, T. (1990) Microsequencing of proteins electrotransferred onto immobilizing matrices from polyaerylamide gel electrophoresis: Application to an insoluble protein. Electrophoresis 11, 573-580
    • (1990) Electrophoresis , vol.11 , pp. 573-580
    • Hirano, H.1    Watanabe, T.2
  • 3
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    • Internal amino acid sequence analysis of proteins separated by one- Or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose
    • Aebersold, R.H., Leavitt, J., Saavedra, R.A., Hood, L.E., and Kent, S.B.H. (1987) Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Proc. Natl. Acad. Sci. USA 84, 6970-6974
    • (1987) Proc. Natl. Acad. Sci. USA , vol.84 , pp. 6970-6974
    • Aebersold, R.H.1    Leavitt, J.2    Saavedra, R.A.3    Hood, L.E.4    Kent, S.B.H.5
  • 4
    • 0027305020 scopus 로고
    • Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography
    • Cohen, S.A. and Michaud, D.P. (1993) Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography. Anal. Biochem. 211, 279-287
    • (1993) Anal. Biochem. , vol.211 , pp. 279-287
    • Cohen, S.A.1    Michaud, D.P.2
  • 5
    • 0027427324 scopus 로고
    • Purification and characterization of a 38-kDa protein, sp38, with zona pellucida-binding property from porcine epididymal sperm
    • Mori, E., Iwamatsu, A., and Mori, T. (1993) Purification and characterization of a 38-kDa protein, sp38, with zona pellucida-binding property from porcine epididymal sperm. Biochem. Biophys. Res. Commun. 196, 196-202
    • (1993) Biochem. Biophys. Res. Commun. , vol.196 , pp. 196-202
    • Mori, E.1    Iwamatsu, A.2    Mori, T.3
  • 9
    • 0028064782 scopus 로고
    • Purification and characterization of a protein that binds to metal responsive elements of the human metallothionein IIA gene
    • Otsuka, F., Iwamatsu, A., Suzuki, K., Ohsawa, M., Hamer, D.H., and Koizumi, S. (1994) Purification and characterization of a protein that binds to metal responsive elements of the human metallothionein IIA gene. J. Biol. Chem. 269, 23700-23707
    • (1994) J. Biol. Chem. , vol.269 , pp. 23700-23707
    • Otsuka, F.1    Iwamatsu, A.2    Suzuki, K.3    Ohsawa, M.4    Hamer, D.H.5    Koizumi, S.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.