Biochemical characterization of mouse liver IBE1-Amide immunoreactivity: Limitations of antibody-based approaches for verification of peptide expression

Peptides

Volumn 17, Issue 5, 1996, Pages 881-883

  Quinn, Kathryn A ^a,b   Unsworth, Edward J ^a   Miller, Mae Jean ^a   Mulshine, James L ^a   Cuttitta, Frank ^a,c  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

^c NONE   (United States)

Author keywords

Hemoglobin; Immunoblotting; Insulin like growth factor I; Mouse; Peptide analysis

Indexed keywords

PEPTIDE; SOMATOMEDIN C;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN EXPRESSION; ARTICLE; GENE EXPRESSION; HEMOGLOBIN ANALYSIS; IMMUNOBLOTTING; IMMUNOREACTIVITY; LIVER; MOUSE; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL;

ANIMALIA; GALLUS GALLUS; OVIS ARIES;

EID: 0029737601     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/0196-9781(96)00103-9     Document Type: Article

References (6)

1. 1. Bell, G. I.; Stempien, M. M.; Fong, N. M.; Rall, L. B. Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors. Nucleic Acids Res. 14:7873-7882; 1986.
2. 2. Popp, R. A.; Bailiff, E. G.; Skow, L. C.; Whitney, J. B., III. The primary structure of genetic variants of mouse hemoglobin. Biochem. Genet. 20:199-208; 1982.
3. 3. Rotwein, P. Two insulin-like growth factor I messenger RNAs are expressed in human liver. Proc. Natl. Acad. Sci. USA 83:77-81; 1986.
4. 4. Rotwein, P.; Folz, R. J.; Gordon, J. I. Biosynthesis of human insulin-like growth factor I (IGF-I). J. Biol. Chem. 262:11807-11812; 1987.
5. 5. Schagger, H.; Von Jagow, G. Tricine-sodium dodecyl sulfate-poly-acrylamide gel electrophoresis for the separation of proteins in the range 1-100 kDa. Anal. Biochem. 166:368-379; 1987.
6. 6. Siegfried, J. M.; Kasprzyk, P. G.; Treston, A. M.; Mulshine, J. L.; Quinn, K. A.; Cuttitta, F. A mitogenic peptide amide encoded within the E peptide domain of the insulin-like growth factor IB prohormone. Proc. Natl. Acad. Sci. USA 89:8107-8111; 1993.