Factors involved in the activation of pre-mRNA splicing from downstream splicing enhancers

Journal of Biochemistry

Volumn 120, Issue 1, 1996, Pages 53-60

  Achsel, Tilmann ^a,b   Shimura, Yoshiro ^a,c  

^a KYOTO UNIVERSITY   (Japan)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c BIOMOL ENG RESEARCH INSTITUTE   (Japan)

Author keywords

Exonic enhancer; Pre mRNA splicing; SR proteins; Transacting factors; U1 snRNP

Indexed keywords

MESSENGER RNA PRECURSOR; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; ENHANCER REGION; EXON; HELA CELL; HUMAN; HUMAN CELL; INTRON; RNA SPLICING;

MAMMALIA;

EID: 0029736853     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021393     Document Type: Article

References (53)

1. Ruskin, B., Zamore, P.D., and Green, M.R. (1988) A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly. Cell 52, 207-219
2. Zamore, P.D. and Green, M.R. (1989) Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. Proc. Natl. Acad. Sci. USA 86, 9243- 9247
3. Zamore, P.D. and Green, M.R. (1991) Biochemical characterization of U2 snRNP auxiliary factor: An essential pre-mRNA splicing factor with a novel intranuclear distribution. EMBO J. 10, 207-214
4. Ge, H. and Manley, J.L. (1990) A protein factor, ASF, controls cell specific alternative splicing of SV40 early pre-mRNA in vitro. Cell 62, 25-34
5. Mayeda, A. and Krainer, A.R. (1992) Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2. Cell 68, 365-375
6. Mayeda, A., Helfman, D.M., and Krainer, A.R. (1993) Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein and pre-mRNA splicing factor SF2/ASF. Mol. Cell. Biol. 13, 2993-3001
7. Fu, X.-D., Mayeda, M., Maniatis, T., and Krainer, A.R. (1992) The general splicing factors SF2 and SC35 have equivalent activities in vitro and both affect alternative splice site selection. Proc. Natl. Acad. Sci. USA 89, 11224-11228
8. Zahler, A.M., Neugebauer, K.M., Lane, W.S., and Roth, M.B. (1993) Distinct functions of SR proteins in alternative pre-mRNA splicing. Science 260, 219-222
9. Fu, X.-D. and Maniatis, T. (1990) Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343, 437-441
10. Krainer, A.R., Mayeda, A., Kozak, D., and Binns, G. (1991) Functional expression of cloned human splicing factor SF2: Homology to RNA binding proteins, U1 70k, and Drosophila splicing regulators. Cell 66, 383-394
11. Roth, M.B., Zahler, A.M., and Stalk, J.A. (1991) A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription. J. Cell Biol. 115, 587-596
12. Ge, H., Zuo, P., and Manley, J.L. (1991) Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators. Cell 66, 373-382
13. Fu, X.-D. and Maniatis, T. (1992) Isolation of a complementary DNA that encodes the mammalian splicing factor SC35. Science 256, 535-538
14. Zahler, A.M., Neugebauer, K.M., Stalk, J.A., and Roth, M.B. (1993) Human SR proteins and isolation of a cDNA encoding SRp75. Mol. Cell. Biol. 13, 4023-4028
15. Zamore, P.D., Patton, J.G., and Green, M.R. (1992) Cloning and domain structure of the mammalian splicing factor U2AF. Nature 355, 609-614
16. Zhang, M., Zamore, P.D., Carmo-Foncesa, M., Lamond, A.I., and Green, M.R. (1992) Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proc. Natl. Acad. Sci. USA 89, 8769-8773
17. Query, C.C., Bentley, R.C., and Keene, J.D. (1989) A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70k U1 snRNP protein. Cell 57, 89-101
18. Mancebo, R., Lo, P.C.H., and Mount, S.M. (1990) Structure and expression of the Drosophila melanogaster gene for the U1 small nuclear ribonucleoprotein particle 70K protein. Mol. Cell. Biol. 10, 2492-2502
19. Caceres, J.F. and Krainer, A.R. (1993) Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains. EMBO J. 12, 4715-4726
20. Zuo, P. and Manley, J.L. (1993) Functional domains of the human splicing factor ASF/SF2. EMBO J. 12, 4727-4737
21. Wu, J.Y. and Maniatis, T. (1993) Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell 75, 1061-1070
22. Ono, Y., Ohno, M., and Shimura, Y. (1994) Identification of a putative ENA helicase (HEH1), a putative human homolog of yeast Prp22. Mol. Cell. Biol. 14, 7611-7620
23. Amrein, H., Hedley, M.L., and Maniatis, T. (1994) The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by Transformer 2. Cell 76, 735-746
24. Kohtz, J.D., Jamison, S.F., Will, C.L., Zuo, P., Lührmann, R., Garcia-Blanco, M.A., and Manley, J.L. (1994) Protein-protein interactions and 5′ splice site recognition in mammalian pre- mRNA precursors. Nature 368, 119-124
25. Kuo, H.-C., Nasim, F.-U.H., and Grabowski, P.J. (1991) Control of alternative splicing by the differential binding of U1 small nuclear ribonucleoprotein particle, Science 251, 1045-1050
26. Robberson, B.L., Cote, G.J., and Berget, S.M. (1990) Exon definition may facilitate splice site selection in RNAs with multiple exons. Mol. Cell. Biol. 10, 84-94
27. Hoffman, B.E. and Grabowski, P.J. (1992) U1 snRNP targets an essential splicing factor, U2AF65, to the 3′ splice site by a network of interactions spanning the exon. Genes Dev. 6, 2554- 2568
28. Watakabe, A., Tanaka, K., and Shimura, Y. (1993) The role of exon sequences in splice site selection. Genes Dev. 7, 407-418
29. Xu, R., Teng, J., and Cooper, T.A. (1993) The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element. Mol. Cell. Biol. 13, 3660-3674
30. Lavigueur, A., La Branche, H., Kornblihtt, A.R., and Chabot, B. (1993) A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding. Genes Dev. 7, 2405-2417
31. Tanaka, K., Watakabe, A., and Shimura, Y. (1994) Polypurine sequences within a downstream exon function as a splicing enhancer. Mol. Cell. Biol. 14, 1347-1354
32. Sun, Q., Mayeda, A., Hampson, R.K., Krainer, A.R., and Rottman, F.M. (1993) General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes Dev. 7, 2598-2608
33. Zahler, A.M., Lane, W.S., Stolk, J.A., and Roth, M.B. (1992) SR proteins: A conserved family of pre-mRNA splicing factors. Genes Dev. 6, 837-847
34. Dignam, J.D., Lebovitz, R.M., and Roeder, R.G. (1983) Accurate transcription initiation by polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475-1489
35. Moore, M.J. and Sharp, P.A. (1992) Site-specific modification of pre-mRNA: The 2′ hydroxyl groups at the splice sites. Science 256, 992-997
36. Sakamoto, H., Ohno, M., Yasuda, K., Mizumoto, K., and Shimura, Y. (1987) In vitro splicing of a chicken δ-crystallin pre-mRNA in a mammalian extract. J. Biochem. 102, 1289-1301
37. Bennett, M., Piñol-Roma, S., Staknin, D., Dreyfuss, G., and Reed, R. (1992) Differential binding of heterogenous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro. Mol. Cell. Biol. 12, 3165-3175
38. Michaud, S. and Reed, R. (1993) A functional association between the 5′ and 3′ splice sites in the earliest pre-spliceosome complex (E) in mammals. Genes Dev. 7, 1008-1020
39. Fu, X.-D. (1993) Specific commitment of different pre-mRNAs to splicing by single SR-proteins. Nature 365, 82-85
40. Staknis, D. and Reed, R. (1994) SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex. Mol. Cell. Biol. 14, 7670-7682
41. Eperon, I.C., Ireland, D.C., Smith, H.A., Mayeda, A., and Krainer, A.R. (1993) Pathways for selection of 5′ splice sites by U1 snRNPs and SF2/ASF. EMBO J. 12, 3607-3617
42. Tian, M. and Maniatis, T. (1993) A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA. Cell 74, 105-114
43. Tacke, R. and Manley, J.L. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14, 3540-3551
44. Tian, M. and Maniatis, T. (1994) A splicing enhancer exhibits both constitutive and regulated activities. Genes Dev. 8, 1703- 1712
45. Zuo, P. and Manley, J.L. (1994) The human splicing factor ASF/ SF2 can specifically recognize pre-mRNA 5′ splice sites. Proc. Natl. Acad. Sci. USA 91, 3363-3367
46. Konforti, B.B. and Konarska, M.M. (1994) U4/U5/U6 snRNP recognizes the 5′ splice site in the absence of U2 snRNP. Genes Dev. 8, 1962-1973
47. Crispino, J.D., Blencowe, B.J., and Sharp, P.A. (1994) Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science 265, 1866-1869
48. Tarn, W.-Y. and Steitz, J.A. (1994) SR proteins can compensate for the loss of U1 snRNP functions in vitro. Genes Dev. 8, 2704- 2717
49. Caceres, J.F., Stamm, S., Helfman, D.M., and Krainer, A.R. (1994) Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science 265, 1706-1710
50. 3 proteins of heterogeneous nuclear ribonucleoparticles modulate 5′ splice site selection in vivo. Proc. Natl. Acad. Sci. USA 91, 6924-6928
51. Swanson, M.S. and Dreyfuss, G. (1988) RNA binding specificity of hnRNP proteins : A subset binds to the 3′ end of introns. EMBO J. 7, 3519-3529
52. Burd, C.G. and Dreyfuss, G. (1994) RNA binding specificity of hnRNP A1: Significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13, 1197-1204
53. Jamison, S.F., Crow, A., and Garcia-Blanco, M.A. (1992) The spliceosome assembly pathway in mammalian extracts. Mol. Cell. Biol. 12, 4279-4287