Purification and characterization of cell growth factor in bovine colostrum

Journal of Veterinary Medical Science

Volumn 58, Issue 1, 1996, Pages 47-53

  Kishikawa, Yoshihiro ^a   Watanabe, Toshi ^a   Watanabe, Tasuku ^a   Kubo, Shuichiro ^a  

^a NIHON UNIVERSITY   (Japan)

Author keywords

Bovine colostrum; Calf kidney epithelial cell; Characterization; Epithelial growth factor; Purification

Indexed keywords

BOVINAE;

AMINO ACID; DNA; GROWTH PROMOTOR; SOMATOMEDIN C;

ANIMAL; ARTICLE; BIOASSAY; BIOSYNTHESIS; BREAST MILK; CATTLE; CELL DIVISION; CELL LINE; CELL STRAIN 3T3; CHEMISTRY; COLOSTRUM; COMPARATIVE STUDY; DOG; DRUG EFFECT; FEMALE; GEL CHROMATOGRAPHY; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KIDNEY; MOUSE; NEWBORN; RAT; TIME;

3T3 CELLS; AMINO ACIDS; ANIMALS; ANIMALS, NEWBORN; BIOLOGICAL ASSAY; CATTLE; CELL DIVISION; CELL LINE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; COLOSTRUM; DNA; DOGS; FEMALE; GROWTH SUBSTANCES; HUMANS; INSULIN-LIKE GROWTH FACTOR I; KIDNEY; MICE; MILK, HUMAN; RATS; TIME FACTORS;

EID: 0029691749     PISSN: 09167250     EISSN: None     Source Type: Journal    
DOI: 10.1292/jvms.58.47     Document Type: Article

References (22)

1. Andrews, P. 1965. The gel-filtration behavior of protein related to their molecular weights over a wide range. Biochem. J. 96: 596-606.
2. Burwen, S, J. and Jones. A. L. 1987. The association of polypeptide hormones and growth factors with the nuclei of target cells. Trends Biochem. Sci. 12: 159-162.
3. 125I-labeled epidermal growth factor to human fibroblasts. J. Biol. Chem. 250: 4297-4304.
4. 125I-Labeled human epidermal growth factor. J. Cell. Biol. 71: 159-171.
5. Carpenter, G. and Cohen, S. 1979. Epidermal growth factor. Ann. Rev. Biochem. 48: 193-216.
6. Carpenter, G. 1980. Epidermal growth factor is a major growth-promoting agent in human milk. Science 210: 198-199.
7. Cohen, S., Carpenter, S., and King, L. Jr. 1980. Epidermal growth factor-receptor-protein kinase interaction: Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity. J. Biol. Chem. 255: 4834-4842.
8. Frances, G. L., Read, L. C., Ballard, J., Bagley, C. J., Upton, F. M., Gravestock, P. M., and Wallace, J. C. 1986. Purification and partial sequence analysis of insulin-like growth factor-1 from bovine colostrum. Biochem. J. 233: 207-213.
9. Hirata, Y. 1983. Human epidermal growth factor in milk. Jpn. J. Endocrinol. 59: 601-606. (in Japanese)
10. Klagsbrun, M. 1978. Human milk stimulates DNA synthesis and cellular proliferation in cultured fibroblasts. Proc Natl. Acad. Sci. U.S.A. 75: 5057-5061.
11. Klagsbrun, M. 1980. Bovine colostrum supports the serum-free proliferation of epithelial cells but not of fibroblasts in long-term culture. J. Cell. Biol. 84: 808-814
12. King, T. P. 1979. Immunochemical properties of some atopic allergens. J. Allergy Clin. Immunol. 64: 159-163.
13. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature (Lond.) 74: 680-685.
14. Lowe, C. R. and Pearson, J. C. 1984, Affinity chromatography on immobilized dyes. Methods Enzymol. 104: 97-113.
15. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
16. Moor, A. T. 1972. Amino acid analysis, p. 629. In: Chemistry and Biology of Peptides (Meinhober, ed.), Arbor Sci. Publisher, U. S. A.
17. Savage, C. R. Jr., Inagami, T., and Cohen, S. 1972. The primary structure of epidermal growth factor. J. Biol. Chem. 247: 7612-7621.
18. Shing, Y., Davison, S., and Klagsbrun, M. 1987. Purification of polypeptide growth factors from milk. Methods Enzymol. 146: 42-48.
19. Suzuki, T. and Katoh, N. 1990. Cysteine proiease in bovine milk capable of hydrolyzing casein as the substrate and elevation of the activity during the course of mastitis. Jpn. J. Vet. Sci. 52: 947-954.
20. Thompson, S. T., Cass, K. H., and Stellwagen, E. 1975. Blue dextran-Sepharose: An affinity column for the dinucleotide fold in proteins. Proc. Natl. Acad. Sci. U.S.A. 72: 669-672.
21. Vesterberg, O. 1971. Isoelectric focusing of proteins. Methods Enzymol. 22: 389-412.
22. Wilson, C. M. 1979. Studies and critique of amido black 10B, coomassie blue R and fast green FCF as stains for protein after polyacrylamide gel electrophoresis. Anal. Biochem. 96: 268-278.