Demonstration and Characterization of a Transport System Capable of Lysine and Leucine Absorption That Is Encoded for in Porcine Jejunal Epithelium by Expression of mRNA in Xenopus laevis Oocytes

Journal of Animal Science

Volumn 74, Issue 1, 1996, Pages 127-137

  Matthews, J C ^a,b   Wong, E A ^a   Bender, P K ^a   Webb Jr , K E ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^b UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

Absorption; Jejunum; Leucine; Lysine; Nutrient Transport; Pigs

Indexed keywords

ORYCTOLAGUS CUNICULUS; SUIDAE; SUS SCROFA; XENOPUS LAEVIS;

COMPLEMENTARY DNA; LEUCINE; LYSINE; MESSENGER RNA;

ANIMAL; ARTICLE; CHEMISTRY; DRUG EFFECT; EPITHELIUM; FEMALE; GENETICS; INTESTINE ABSORPTION; JEJUNUM; MALE; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OOCYTE; PHYSIOLOGY; SWINE; TIME; XENOPUS LAEVIS;

ANIMALS; BASE SEQUENCE; DNA, ANTISENSE; EPITHELIUM; FEMALE; INTESTINAL ABSORPTION; JEJUNUM; LEUCINE; LYSINE; MALE; MOLECULAR SEQUENCE DATA; OOCYTES; RNA, MESSENGER; SWINE; TIME FACTORS; XENOPUS LAEVIS;

EID: 0029688657     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/1996.741127x     Document Type: Article

References (37)

1. Bertran, J., A. Werner, J. Chillaron, V. Nunes, J. Biber, X. Testar, A. Zorzano, X. Estivill, H. Murer, and M. Palacin. 1993. Expression cloning of a human renal cDNA that induces high affinity transport of L-cystine shared with dibasic amino acids in Xenopus oocytes. J. Biol. Chem. 268:14842.
2. Bertran, J., A. Werner, M. L. Moore, G. Stange, D. Markovich, J. Biber, X. Testar, A. Zorzano, M. Palacin, and H. Murer. 1992. Expression cloning of cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids. Proc. Natl. Acad. Sci. USA 89:5601.
3. Buraczewski, S., A. G. Chamberlain, F. Horszczaruk, and T. Zebrowska. 1970. Lysine and arginine interactions affecting their absorption from the duodenum of the pig. Proc. Nutr. Soc. 29:51A.
4. Burton, K. A., P. S. James, M. W. Smith, and J. D. Young. 1980. Different pathways for lysine transport across neonatal pig intestine. J. Physiol. 308:451.
5. Cheeseman, C. I. 1991. Molecular mechanisms involved in the regulation of amino acid transport. Prog. Biophys. Mol. Biol. 55:71.
6. Coleman, A. 1984. Translation of eukaryotic messenger RNA in Xenopus oocytes. In: B. D. Hames and S. J. Higgins (Ed.) Transcription and Translation. A Practical Approach. IRL Press, Oxford, U.K.
7. D'Mello, J.P.F. 1993. Amino acid supplementation of cereal-based diets for non-ruminants. Anim. Feed Sci. Technol. 45:1.
8. Dumont, J. N. 1972. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J. Morphol. 136:153.
9. Farrell, R. E., Jr. 1993. RNA Methodologies. A Laboratory Guide for Isolation and Characterization. Academic Press, San Diego, CA.
10. Ganapathy, V., M. Brandsch, and F. H. Leibach. 1994. Intestinal transport of amino acids and peptides. In: L. R. Johnson (Ed.) Physiology of the Gastrointestinal Tract (3rd Ed.). p 1773. Raven Press, New York.
11. Hediger, M. A., T. Ikeda, M. Coady, C. B. Gundersen, and E. M. Wright. 1987. Expression of size-selected mRNA encoding the intestinal Na/glucose cotransporter in Xenopus laevis oocytes. Proc. Natl. Acad. Sci. USA 84:2634.
12. James, P. S., M. W. Smith, D. R. Tivey, and T.J.G. Wilson. 1987. Dexamethasone selectively increases sodium-dependent alanine transport across neonatal piglet intestine. J. Physiol. (Camb.) 393:569.
13. Kanai, Y., and M. A. Hediger. 1992. Primary structure and func tional characterization of a high-affinity glutamate transporter. Nature (Lond.) 360:467.
14. Kilberg, M. S., B. R. Stevens, and D. A. Novak. 1993. Recent advances in mammalian amino acid transport. Annu. Rev. Nutr. 13:137.
15. Lawless, K., D. Maenz, and C. Cheeseman. 1987. Is leucine an allosteric modulator of the lysine transporter in the intestinal basolateral membrane? Am. J. Physiol. 253:G637.
16. Leo, W.-S., R. G. Wells, R. V. Sabbag, T. K. Mohandas, and M. A. Hediger. 1993. Cloning and chromosomal localization of a human kidney cDNA involved in cystine, dibasic, and neutral amino acid transport. J. Clin. Invest. 91:1959.
17. Leibholz, J. 1989. The utilization of free and protein-bound lysine. In: M. Friedman (Ed.) The Utilization of Amino Acids. p 175. CRC Press, Boca Raton, FL.
18. Lentner, M., and T. Bishop. 1993. Experimental Design and Analysis. p 90. Valley Book Company, Blacksburg, VA.
19. Lerner, J. 1987. Acidic amino acid transport in animal cells and tissues. Comp. Biochem. Physiol. 87B:443.
20. + amino acid transport activities in Xenopus laevis oocytes. J. Biol. Chem. 267:15384.
21. Miyamoto, Y., Y. G. Thompson, E. F. Howard, V. Ganapathy, and F. H. Leibach. 1991. Functional expression of the intestinal peptide-proton co-transporter in Xenopus laevis oocytes. J. Biol. Chem. 266:4742.
22. Mosckovitz, R., S. Udenfriend, A. Felix, E. Heimer, and S. S. Tate. 1994. Membrane topology of the rat neutral and basic amino acid transporter. FASEB J. 8:1069.
23. Munck, B. G. 1989. Amino acid transport across the hen colon: Interactions between leucine and lysine. Am. J. Physiol. 256:G532.
24. NEC. 1988. Nutrient Requirements of Swine (9th Ed.). National Academy Press, Washington, DC.
25. Ohta, T., K. J. Isselbacher, and D. B. Rhoads. 1990. Regulation of glucose transporters in LLC-PK cells: Effects of D-glucose and monosaccharides. Mol. Cell Biol. 10:6491.
26. Pickel, V. M., M. J. Nirenberg, J. Chan, R. Mosckovitz, S. Udenfriend, and S. S. Tate. 1993. Ultrastructural localization of a neutral and basic amino acid transporter in rat kidney and intestine. Proc. Natl. Acad. Sci. USA 90:7779.
27. Puissant, C., and L.-M. Houdebine. 1990. An improvement of the single-step method of RNA isolation by acid guinidinium thiocyanate-phenol-chloroform extraction. BioTechniques 8: 148.
28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual (2nd Ed). Cold Spring Harbor Press, Cold Spring Harbor, NY.
29. SAS. 1985. User's Guide: Statistics (Version 5 Ed.). SAS Inst. Inc., Cary, NC.
30. SAS. 1988. SAS/STAT® User's Guide (Release 6.03). SAS Inst. Inc., Cary, NC.
31. Stevens, B. R. 1992. Amino acid transport in intestine. In: M. S. Kilberg and D. Haussinger (Ed.) Mammalian Amino Acid Transport. p 149. Plenum Press, New York.
32. Tarnuzzer, R. W., M. J. Campa, N.-X. Qian, E. Englesberg, and M. S. Kilberg. 1990. Expression of the mammalian system A neutral amino acid transporter in Xenopus oocytes. J. Biol. Chem. 265:13914.
33. +-independent neutral amino acid transporter from rat kidney. Proc. Natl. Acad. Sci. USA 89:1.
34. +-independent transport of basic and zwitterionic amino acids in mouse blastocysts by a shared system and by processes which distinguish between those substrates. J. Biol. Chem. 263:3150.
35. Wells, R. G., and M. A. Hediger. 1992. Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases. Proc. Natl. Acad. Sci. USA 89:5596.
36. Wolffram, S., E. Eggenberger, and E. Scharrer. 1986. Kinetics of D-glucose and L-leucine transport into sheep and pig intestinal brush border membrane vesicles. Comp. Biochem. Physiol. 84A: 589.
37. + (CAT-1) amino acid transporter as detected by immunohistochemistry. Am. J. Physiol. 266:E817.