Demonstration by reverse transcription-polymerase chain reaction of multiple cytokine mRNA expression in bovine alveolar macrophages and peripheral blood mononuclear cells

Research in Veterinary Science

Volumn 60, Issue 1, 1996, Pages 94-96

  Ito, T ^a   Kodama, M ^a  

^a NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINAE;

CYTOKINE; MESSENGER RNA; PRIMER DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; BIOSYNTHESIS; CATTLE; GENE EXPRESSION; IMMUNOLOGY; LUNG ALVEOLUS MACROPHAGE; LUNG LAVAGE; LYMPHOCYTE; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ANIMALS; BASE SEQUENCE; BRONCHOALVEOLAR LAVAGE FLUID; CATTLE; CYTOKINES; DNA PRIMERS; GENE EXPRESSION; LYMPHOCYTES; MACROPHAGES, ALVEOLAR; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0029688226     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0034-5288(96)90140-X     Document Type: Article

References (17)

1. BRENNER, C. A., TAM, A. W., NELSON, P. A , ENGLEMAN, E. G , SUZUKI. N., FRY, K. E. & LARRICK, J. W (1989) Message amplification phenotyping (MAPPing): A technique to simultaneously measure multiple mRNAs from small numbers of cells. Bioiechniques 7, 1096-1103
2. CERRETTI, D. P., McKEREGHAM, K., LARSEN, A., COSMAN, D., GILLIS, S & BAKER, P. E. (1986a) Cloning, sequence, and expression of bovine interferon-γ. Journal of Immunology 136, 4561-4564
3. CERRETTI, D. P., McKEREGHAM, K , LARSEN, A., CANTRELL, M. A , ANDERSON, D., GILLIS, S., COSMAN, D. & BAKER, P E. (1986b) Cloning, sequence, and expression of bovine interleukin 2. Proceedings of the National Academy of Sciences, USA 83, 3223-3227
4. CHIRGWIN, J. M., PRZYBYLA, A. E., MACDONALD, R J & RUTTER, W J. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299
5. CLUDTS, I., CLEUTER, Y., BURNY, A. & DROOGMENS, L., (1993) Closing and characterization of the tandemly arranged bovine lymphotoxin and tumor necrosis factor-alpha gene. Cytokine 5, 336-341
6. DEGEN, J. L., NEUBAUER, M. G., DEGEN, S. J F., SEYFRIED, C. E & MORRIS, D. R (1983) Regulation of protein synthesis in mitogen-activated bovine lymphocytes. Journal of Biological Chemistry 258, 12153-12162
7. DROOGMANS, L , CLUDTS, I., CLEUTER, Y, KETTMANN, R. & BURNY, A. (1992) Nucleotide sequence of bovine interleukin-6 CDNA. DNA Sequence 2, 411-413
8. HEUSSLER, V. T., EICHHORN, M. & DOBBELAERE, D. A. E. (1992a) Cloning of a full-length cDNA encoding bovine interleukin 4 by the polymerase chain reaction. Gene 114, 273-278
9. HEUSSLER, V. T., EICHHORN, M., REEVES, R., MAGNUSON, N. S., WILLIAMS, R O. & DOBBELAERE, D. A. E. (1992b) Constitutive IL-2 mRNA expression in lymphocytes infected with the intracellular parasite Theileria parva. Journal of Immunology 149, 562-567
10. ITO, T. & KODAMA, M. (1994) Detection of bovine interleukin-1 alpha and interleukin-1 beta gene expression by reverse transcription-polymerase chain reaction. Veterinary Immunology and Immunopathology 40, 93-103
11. LEONG, S. R., FLAGGS, G. M., LAWMAN, M. & GRAY, P W. (1988a) The nucleotide sequence for the CDNA of bovine interleukin-1 alpha. Nucleic Acids Research 16, 9053
12. LEONG, S. R., FLAGGS, G. M., LAWMAN, M. & GRAY, P. W. (1988b) The nucleotide sequence for the cDNA of bovine interleukin-1 beta. Nucleic Acids Research 16, 9054
13. MALISZEWSKI, C. R., BAKER, P. E., SCHOENBORN, M. A., DAVIS, B. S., COSMAN, D., GILLIS, S. & CERRETTI, D P (1988a) Cloning, sequence and expression of bovine interleukin 1-α and interleukin 1-β complementary DNAs. Molecular Immunology 25, 429-437
14. MALISZEWSKI, C. R., SCHOENBORN, M. A., CERRETTI, D. P , WIGNALL, J. M., PICHA, K. S., COSMAN, D., TUSHINSKI, R. J., GILLIS, S. & BAKER, P. E. (1988b) Bovine GM-CSF: Molecular cloning and biological activity of the recombinant protein. Molecular Immunology 25, 843-850
15. MATHIALAGAN, N., BIXBY, J. A. & ROBERTS, R. M. (1992) Expression of interleukin-6 in porcine, ovine and bovine preimplantation conceptuses. Molecular Reproduction and Development 32, 324-330
16. RAPPOLEE, D. A., MARK, D., BANDA, M J & WERB, Z. (1988) Wound macrophages express TGF-α and other growth factors in vivo: Analysis by mRNA phenotyping. Science 241, 708-712
17. REEVES, R , SPIES, A. G., NISSEN, M. S., BUCK, C. D., WEINBERG, A. D., BARR, P. J., MAGNUSON, N. S. & MAGNUSON, J. A. (1986) Molecular cloning of a functional bovine interleukin-2 cDNA. Proceedings of the National Academy of Sciences, USA 83, 3228-3232