Identification of the Chimeric Protein Product of the CBFB-MYHII Fusion Gene in Inv(16) Leukemia Cells

Genes Chromosomes and Cancer

Volumn 16, Issue 2, 1996, Pages 77-87

  Liu, P Paul ^a   Wijmenga, Cisca ^a   Hajra, Amitav ^a   Blake, Trevor B ^a   Kelley, Christine A ^b   Adelstein, Robert S ^b   Bagg, Adam ^c   Rector, James ^d   Cotelingam, James ^d   Willman, Cheryl L ^e   Collins, Francis S ^a  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^c GEORGETOWN UNIVERSITY MEDICAL CENTER   (United States)

^d WALTER REED ARMY MEDICAL CENTER   (United States)

^e UNIVERSITY OF NEW MEXICO SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN; COMPLEMENTARY DNA; HYBRID PROTEIN; MYOSIN HEAVY CHAIN; POLYCLONAL ANTIBODY; RNA; TRANSCRIPTION FACTOR;

ACUTE GRANULOCYTIC LEUKEMIA; ADULT; AGED; ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL STRAIN 3T3; CHROMOSOME 16; CHROMOSOME INVERSION; CONTROLLED STUDY; DNA PROTEIN COMPLEX; ELECTROPHORETIC MOBILITY; FEMALE; GENE FUSION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; LEUKEMIA CELL; MALE; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

3T3 CELLS; ADULT; AGED; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELL LINE, TRANSFORMED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; LEUKEMIA, MYELOCYTIC, ACUTE; MALE; MICE; MICE, NUDE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; ONCOGENE PROTEINS, FUSION; POLYMERASE CHAIN REACTION;

EID: 0029680098     PISSN: 10452257     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1098-2264(199606)16:2<77::aid-gcc1>3.0.co;2-%23     Document Type: Article

References (38)

1. Babij P, Periasamy M (1989) Myosin heavy chain isoform diversity in smooth muscle is produced by differential RNA processing. J Mol Biol 210:673-679.
2. Bae S-C, Ogawa E, Maruyama M, Oka H, Satake M, Shigesada K, Jenkins NA, Gilbert DJ, Copeland NG, Ito Y (1994) PEBP2αB/ mouse AML1 consists of multiple isoforms that possess differential transactivation potentials. Mol Cell Biol 14:3242-3252.
3. Claxton DF, Liu P, Hsu HB, Marlton P, Hester J, Collins F, Deisseroth AB, Rowley JD, Siciliano MJ (1994) Detection of fusion transcripts generated by the inversion 16 chromosome in acute myelogeous leukemia. Blood 83:1750-1756.
4. Eddinger TJ, Murphy RA (1988) Two smooth muscle myosin heavy chains differ in their light meromyosin fragment. Biochemistry 27:3807-3811.
5. Evers JP, Bagg A, Himoe E, Zwiebel JA, Jacobson RJ (1992) Temporal association of marrow eosinophilia with inversion of chromosome 16 in recurrent blast crises of chronic myelogenous leukemia. Cancer Genet Cytogenet 62:134-139.
6. Gumucio DL, Rood KL, Gray TA, Riordan MF, Sartor CI, Collins FS (1988) Nuclear proteins that bind the human γ-globin promoter: Alterations in binding produced by point mutations associated with hereditary persistence of fetal hemoglobin. Mol Cell Biol 8:5310-5322.
7. Hajra A (1995) Transformation properties of the leukemic CBFβ-SMMHC chimeric protein. Dissertation, University of Michigan, Ann Arbor, MI, p 157.
8. Hajra A, Liu PP, Wang Q, Kelley CA, Stacy T, Adelstein RS, Speck NA, Collins FS (1995a) The leukemic core binding factor β-smooth muscle myosin heavy chain (CBFβ-SMMHC) chimeric protein requires both CBFβ and myosin heavy chain domains for transformation of NIH 3T3 cells. Proc Natl Acad Sci USA 92: 1926-1930.
9. Hajra A, Liu PP, Speck NA, Collins FS (1995b) Overexpression of CBFα reverses cellular transformation by the CBFβ-SMMHC chimeric oncoprotein. Mol Cell Biol 15:4980-4989.
10. Hébert J, Cayuela J-M, Daniel M-T, Berger R, Sigaux F (1994) Detection of minimal residual disease in acute myelomonocytic leukemia with abnormal marrow eosinophils by nested polymerase chain reaction with allele specific amplification. Blood 84:2291-2296.
11. Hodge TP, Cross R, Kendrick-Jones J (1992) Role of the COOH-terminal nonhelical tailpiece in the assembly of a vertebrate non-muscle myosin rod. J Cell Biol 118:1085-1095.
12. Kawamoto S, Adelstein RS (1987) Characterization of myosin heavy chains in cultured aorta smooth muscle cells. A comparative study. J Biol Chem 262:7282-7288.
13. Kelley CA, Adelstein RS (1990) The 204-kDa smooth muscle myosin heavy chain is phosphorylated in intact cells by casein kinase II on a serine near the carboxyl terminus. J Biol Chem 265:17876-17882.
14. Kelley CA, Sellers JR, Goldsmith PK, Adelstein RS (1992) Smooth muscle myosin is composed of homodimeric heavy chains. J Biol Chem 267:2127-2130.
15. Kuro-o M, Nagai R, Nakahara K, Katoh H, Tsai RC, Tsuchimochi H, Yazaki Y, Ohkubo A, Takaku F (1991) cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis. J Biol Chem 266:3768-3773.
16. Larson RA, Williams SF, Le Beau MM, Bitter MA, Vardiman JW, Rowley JD (1986) Acute myelomonocytic leukemia with abnormal eosinophils and inv(16) or t(16;16) has a favorable prognosis. Blood 68:1242-1249.
17. Le Beau MM, Larson RA, Bitter MA, Vardiman JW, Golomb HM, Rowley JD (1983) Association of an inversion of chromosome 16 with abnormal marrow eosinophils in acute myelomonocytic leukemia. N Engl J Med 309:630-636.
18. Levanon D, Negreanu V, Bernstein Y, Bar-Am I, Avivi L, Groner Y (1994) AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization. Genomics 23:425-432.
19. Liu P, Perryman MB, Liao W, Siciliano MJ (1992) Efficiency and limitations of the hn-cDNA library approach for the isolation of human transcribed genes from hybrid cells. Somatic Cell Mol Genet 18:7-18.
20. Liu P, Claxton DF, Marlton P, Hajra A, Siciliano J, Freedman M, Chandrasekharappa SC, Yanagisawa K, Stallings RL, Collins FS, Siciliano MJ (1993a) Identification of yeast artificial chromosomes containing the inversion 16 p-arm breakpoint associated with acute myelomonocytic leukemia. Blood 82:716-721.
21. Liu P, Tarlé SA, Hajra A, Claxton DF, Marlton P, Freedman M, Siciliano MJ, Collins FS (1993b) Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia. Science 261:1041-1044.
22. Liu P, Seidel N, Bodine D, Speck N, Tarlé S, Collins FS (1994) Acute myeloid leukemia with inv(16) produces a chimeric transcription factor with a myosin heavy chain tail. Cold Spring Harbor Symp Quant Biol 59:547-553.
23. Liu PP, Hajra A, Wijmenga C, Collins FS (1995) Molecular pathogenesis of the chromosome 16 inversion in the M4Eo subtype of AML. Blood 85:2289-2302.
24. Matsuoka R, Yoshida MC, Furutani Y, Imamura S, Kanda N, Yanagisawa M, Masaki T, Takao A (1993) Human smooth muscle myosin heavy chain gene mapped to chromosomal region 16q12. Am J Med Genet 46:61-67.
25. Mohammad MA, Sparrow MP (1989) The distribution of heavychain isoforms of myosin in airways smooth muscle from adult and neonate humans. Biochem J 260:421-426.
26. Nagai R, Kuro-o M, Babij P, Periasamy M (1989) Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis. J Biol Chem 264:9734-9737.
27. Poirel H, Radford-Weiss I, Rack K, Troussard X, Veil A, Valensi F, Picard F, Guesnu M, Leb{{oe}}uf D, Melle J, Dreyfus F, Flandrin G, Macintyre E (1995) Detection of the chromosome 16 CBFβ-MYH11 fusion transcript in myelomonocytic leukemias. Blood 85:1313-1322.
28. Rovner AS, Thompson MM, Murphy RA (1986a) Two different heavy chains are found in smooth muscle myosin. Am J Physiol 250:C861-870.
29. Rovner AS, Murphy RA, Owens GK (1986b) Expression of smooth muscle and nonmuscle myosin heavy chains in cultured vascular smooth muscle cells. J Biol Chem 261:14740-14745.
30. Sakakura C, Yamagushi-Iwai Y, Satake M, Bae S, Takahashi A, Ogawa E, Hagiwara A, Takahashi T, Murakami A, Makino K, Nakagawa T, Kamada N, Ito Y (1994) Growth inhibition and induction of differentiation of t(8;21) acute myeloid leukemia cells by the DNA-binding domain of PEBP2 and the AML1/ MTG8(ETO)-specific antisense oligonucleotide. Proc Natl Acad Sci USA 91:11723-11727.
31. Schurloff SA, Meyers S, Hiebert SW, Raimondi SC, Head DR, Willman CL, Wolman S, Slovak ML, Carroll AJ, Behm F, Hulshof MG, Motroni TA, Okuda T, Liu P, Collins FS, Downing JR (1995) Heterogeneity in CBFβ/MYH11 fusion messages encoded by the inv(16)(p13q22) and the t(16;16)(p13;q22) in acute myelogenous leukemia. Blood 85:3695-3703.
32. Tanaka T, Mitani K, Kurokawa M, Ogawa S, Tanaka K, Nishida J, Yazaki Y, Shibata Y, Hirai H (1995) Dual functions of the AML1/ Evi-1 chimeric protein in the mechanism of leukemogenesis in t(3;21) leukemias. Mol Cell Biol 15:2383-2392.
33. Testa JR, Hogge DE, Misawa S, Zandparsa N (1984) Chromosome 16 rearrangements in acute myelomonocytic leukemia with abnormal eosinophils. N Engl J Med 310:468-469.
34. Tsao AE, Eddinger TJ (1993) Smooth muscle myosin heavy chains combine to form three native myosin isoforms. Am J Physiol 264: H1653-H1662.
35. van der Reijden BA, Lombardo M, Dauwerse HG, Giles RH, Mühlematter D, Bellomo MJ, Wessels HW, Beverstock GC, van Ommen G-JB, Hagemeijer A, Breuning MH (1995) RT-PCR diagnosis of patients with acute nonlymphocytic leukemia and inv(16)(p13q22) and identification of new alternative splicing in CBFB-MYH11 transcripts. Blood 86:277-282.
36. Wang S, Speck NA (1992) Purification of core-binding factor, a protein that binds the conserved core site in murine leukemia virus enhancers. Mol Cell Biol 12:89-102.
37. Wijmenga C, Gregory PE, Hajra A, Schröck E, Ried T, Eils R, Liu PP, Collins FS (1995) The CBFβ-SMMHC chimeric protein involved in acute myeloid leukemia forms novel nuclear rod-like structures in transformed NIH 3T3 cells. Proc Natl Acad Sci USA (in press).
38. Yanagisawa K, Horiuchi T, Fujita S (1991) Establishment and characterization of a new human leukemia cell line derived from M4Eo. Blood 78:451-457.