Replacement of Trp28 in Escherichia coli thioredoxin by site-directed mutagenesis affects thermodynamic stability but not function

Journal of Biological Chemistry

Volumn 271, Issue 6, 1996, Pages 3091-3096

  Slaby, Ivan ^a   Cerna, Vaclava ^a   Jeng, Mei Fen ^b   Dyson, H Jane ^b   Holmgren, Arne ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

GUANIDINE; GUANIDINE DERIVATIVE; PRIMER DNA; RECOMBINANT PROTEIN; THIOREDOXIN; TRYPTOPHAN; ALANINE; DISULFIDE; DITHIOL DERIVATIVE; DNA POLYMERASE; INSULIN; MUTANT PROTEIN; THIOREDOXIN REDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COMPARATIVE STUDY; DRUG STABILITY; ESCHERICHIA COLI; KINETICS; METABOLISM; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEOTIDE SEQUENCE; POINT MUTATION; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; SPECTROFLUOROMETRY; THERMODYNAMICS; BACTERIOPHAGE; CIRCULAR DICHROISM; ELECTRON TRANSPORT; ENVIRONMENTAL TEMPERATURE; FLUORESCENCE SPECTROSCOPY; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STABILITY; PROTON NUCLEAR MAGNETIC RESONANCE; REDUCTION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CONSERVED SEQUENCE; DNA PRIMERS; DRUG STABILITY; ESCHERICHIA COLI; GUANIDINE; GUANIDINES; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; THIOREDOXIN; TRYPTOPHAN;

EID: 0029671072     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.6.3091     Document Type: Article

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