Characterization of an RTX toxin from enterohemorrhagic Escherichia coli O157:H7

Infection and Immunity

Volumn 64, Issue 1, 1996, Pages 167-175

  Bauer, Margaret E ^a   Welch, Rodney A ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; EHEC HLYA PROTEIN, E COLI; EHEC-HLYA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; HEMOLYSIN; HLYA PROTEIN, E COLI; CALCIUM; CHROMIUM 51; CYTOTOXIN; ESCHERICHIA COLI ENDOTOXIN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY A10; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; B CELL LYMPHOMA; CATTLE; CELL CULTURE; CLASSIFICATION; COMPARATIVE STUDY; CROSS REACTION; DOSE RESPONSE; ESCHERICHIA COLI; GENETICS; HEMOLYSIS; HUMAN; IMMUNOBLOTTING; IMMUNOLOGY; PATHOGENICITY; PHYLOGENY; PLASMID; TOXICITY TESTING; ANIMAL CELL; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; PATHOPHYSIOLOGY; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SHEEP; STRAIN DIFFERENCE; TOXIN ANALYSIS; VIRULENCE;

ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CATTLE; CROSS REACTIONS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEMOLYSIN PROTEINS; HEMOLYSIS; HUMANS; IMMUNOBLOTTING; LYMPHOMA, B-CELL; PHYLOGENY; PLASMIDS; TOXICITY TESTS; TUMOR CELLS, CULTURED;

EID: 0029671024     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.1.167-175.1996     Document Type: Article

References (42)

1. Bauer, M. E., and R. A. Welch. Unpublished observations.
2. Burrows, L. L., and R. Y. C. Lo. 1992. Molecular characterization of an RTX toxin determinant from Actinobacillus suis. Infect. Immun 60:2166-2173.
3. Chang, Y., D. Ma, J. Shi, and M. M. Chengappa. 1993. Molecular characterization of a leukotoxin gene from a Pasteurella haemolytica-like organism, encoding a new member of the RTX toxin family. Infect Immun 61:2089-2095.
4. Chang, Y.-F., R. Young, and D. K. Struck. 1991 The Actinobacillus pleuropneumoniae hemolysin determinant: unlinked appCA and appBD loci flanked by pseudngenes. J. Racteriol. 173:5151-5158.
5. Confer, A. W., K. R. Simons, M. T. Barrie, and K. D. Clinkenbeard. 1990. Effects of Pasteurella haemolyitica leukotoxin on neutrophils from whitetailed deer and several exotic ruminant species. Vet. Res. Commun. 14:175-180.
6. Dytoc, M., R. Soni, F. Cockerill III, J. de Azavedo, M. Louie, J. Brunton, and P. Sherman. 1993 Multiple determinants of verotoxin-producing Escherichia coli O157:H7 attachment-effacement. Infect. Immun. 61:3382-3391.
7. Felmlee, T., S. Pellett, and R. A. Welch. 1985. The nucleotide sequence of an Escherichia coli chromosomal hemolysin. J. Bacteriol. 163:94-105.
8. Forestier, C., and R. A. Welch. 1991 Identification of RTX toxin target cell specificity domains by use of hybrid genes. Infect. Immun. 59:4212-4220
9. Frey, J., R. Meier, D. Gygi, and J. Nicolet. 1991. Nucleotide sequence of the hemolysin I gene from Actinobacillus pleuropneumoniae. Infect. Immun. 59:3026-3032.
10. Gentry, M. J., and S. Srikumaran. 1991 Neutralizing monoclonal antibodies to Pasleurella haemolytica leukotoxin affinity-purify the toxin from crude supernatants Microb. Pathog. 10:411-417.
11. Hewlett, E. L., V. M. Gordon, J. D. McCaffery, W. M. Sutherland, and M. C. Gray. 1989 Adenylate cyclase toxin from Bordetella pertussis: identification and purification of the holotoxin molecule. J. Biol. Chem. 264:19379-19384.
12. Hewlett, E. L., L. Gray, M. Allietta, I. Ehrmann, V. M. Gordon, and M. C. Gray. 1991 Adenylate cyclase toxin from Bordetella pertussis: conformational change associated with toxin activity. J. Biol. Chem. 266:17503-17508.
13. Highlander, S. K., M. Chidarabaram, M. J. Engler, and G. M. Weinstock. 1989. DNA sequence of the Pasteurella haemolytica leukotoxin gene cluster DNA 8:15-28.
14. Hugo, F., M. Arvand, J. Reichwein, N. Mackman, I. B. Holland, and S. Bhakdi. 1987. Identification of monoclonal antibodies of hemolysin produced by clinical isolates of Escherichia coli. J. Clin Microbiol 25:26-30.
15. Karch, H., J. Heesemann, R. Laufs, A. D. O'Brien, C. O. Tacket, and M. M. Levine. 1987. A plasmid of enterohemorrhagic Escherichia coli O157:H7 is required for expression of a new fimbrial antigen and for adhesion to epithelial cells. Infect. Immun. 55:455-461.
16. Karmali, M. A. 1989. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 2:15-38.
17. Karmali, M. A., M. Petric, C. Lim, P. C. Fleming, G. S. Arbus, and H. Lior. 1985. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin producing Escherichia coli J. Infect. Dis. 151:775-782.
18. Kolodrubetz, D., T. Dailey, J. Ebersole, and E. Kraig. 1989 Cloning and expression of the leukotoxin gene from Actinobacillus actinomycetemcomutans. Infect. Immun. 57:1465-1469.
19. Leeds, J. A., and R. A. Welch. Unpublished observations.
20. Lo, R. Y. C., C. Strathdee, and P. Shewen. 1987. Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1. Infect Immun. 55:1987-1996
21. 2+-dependent binding to erythrocytes. Mol Gen Genet. 214:553-561.
22. Macdonald, J., and A. N. Rycroft. 1992 Molecular cloning and expression of ptxA, the gene encoding the 120-kilodalton cytotoxin of Actinobacillus pleviopneumomae. serotype 2. Infect. Immun. 60:2726-2732
23. McWhinney, D. R., Y.-F. Chang, R. Young, and D. K. Struck. 1992. Separable domains define target cell specificities of an RTX hemolysin from Actinobacillus pleuropneumoniae. J Bacteriol. 174:291-297.
24. Pellett, S., D. F. Boehm, I. S. Snyder, G. Rowe, and R. A. Welch. 1990. Characterization of monoclonal antibodies against the Escherichia coli hemolysin. Infect. Immun 58:822-827.
25. Rennie, R. P., J. H. Freer, and J. P. Arbuthnott. 1974. The kinetics of erythrocyte lysis by Escherichia coli haemolysin. J. Med. Microbiol 7:189-195.
26. Rowe, G. E., S. Pellett, and R. A. Welch. 1994. Analysis of toxinogenic functions associated with the RTX repeat region and monoclonal antibody D12 epitope of Escherichia coli hemolysin (HlyA) Infect. Immun. 62:579-588
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989 Molecular cloning. a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
28. Schmidt, H., L. Beutin, and H. Karch. 1995. Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933. Infect Immun. 63:1055-1061.
29. Schmidt, H., H. Karch, and L. Beutin. 1994. The large-sized plasmids of enterohemorrhagic Escherichia coli O157 strains encode hemolysins which are presumably members of the E. coli α-hemolysin family. FEMS Microbiol. Lett. 117:189-196.
30. Sherman, P., F. Cockerill III, R. Soni, and J. Brunton. 1991. Outer membranes are competitive inhibitors of Escherichia coli O157:H7 adherence to epithelial cells. Infect. Immun. 59:890-899.
31. Shewen, P. E., and B. N. Wilkie. 1982. Cytotoxin of Pasteurella haemolytica acting on bovine leukocytes Infect Immun 35:91-94.
32. Swihart, K. G. 1990. Molecular characterization of Proteus hemolysins Ph D thesis University of Wisconsin. Madison.
33. Swofford, D. L. 1993. Phytogenetic analysis using parsimony, version 3 0s Illinois Natural History Survey, Champaign, III
34. Swofford, D. L., and G. J. Olsen. 1990 Phytogeny reconstruction, p 411-501 In C Moritz and D. M. Hills (ed.). Molecular systematics. Sinauer Associates. Inc., Sunderland, Mass.
35. Tesh, V. L., and A. D. O'Brien. 1991. The pathogenic mechanisms of Shiga toxin and the Shiga-like toxins Mol. Microbiol. 5:1817-1822.
36. Towbin, H., T. Staehlin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc. Natl. Acad. Sci USA 76:4350-4354
37. Welch, R. A. 1987. Identification of two different hemolysin determinants in uropathogenic Proteus isolates. Infect. Immun. 55:2183-2190.
38. Welch, R. A. 1991 Pore-forming cytolysins of gram-negative bacteria. Mol. Microbiol. 5:521-528.
39. Welch, R. A., M. E. Bauer, A. D. Kent, J. A. Leeds, M. Moayeri, L. B. Regassa, and D. L. Swenson. Battling against host phagocytes: the wherefore of the RTX family of toxins? Infect. Agents Dis , in press.
40. Welch, R. A., E. P. Dellinger, B. Minshew, and S. Falkow. 1981. Hemolysin contributes to virulence of extra-intestinal Escherichia coli infections. Nature (London) 294:665-667.
41. Welch, R. A., and S. Pellett. 1988. Transcriptional organization of the Escherichia coli hemolysin. J Bacteriol 170:1622-1630.
42. Yu, J., and J. B. Kaper. 1992. Cloning and characterization of the eae gene of enterohemorrhagic Escherichia coli O157:H7. Mol Microbiol. 6:411-417.