"Doublex" fluorescent DNA sequencing: Two independent sequences obtained simultaneously in one reaction with internal labeling and unlabeled primers

Analytical Biochemistry

Volumn 234, Issue 2, 1996, Pages 166-174

  Wiemann, Stefan ^a,b   Stegemann, Josef ^a   Zimmermann, Jürgen ^a   Voss, Hartmut ^a   Benes, Vladimir ^a   Ansorge, Wilhelm ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENE ENCODING;

DNA POLYMERASE; DNA SEQUENCING; DNA-TEMPLATE;

DNA SEQUENCES;

LANES;

DEOXYADENOSINE TRIPHOSPHATE; DEOXYCYTIDINE TRIPHOSPHATE; DNA; DNA POLYMERASE; FLUORESCEIN; NUCLEOSIDE TRIPHOSPHATE;

ARTICLE; BINDING SITE; DNA SEQUENCE; DNA TEMPLATE; HUMAN; NONHUMAN; PLASMID; PRIORITY JOURNAL;

EID: 0029670875     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0068     Document Type: Article

References (26)

1. Ansorge, W., Sproat, B., Stegemann, J., and Schwager, C. (1986) J. Biochem. Biophys. Methods 13, 315-323.
2. Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughes, P., Dodd, C., Connel, C. R., Heiner, C., Kent, S. B. H., and Hood, L. E. (1986) Nature 321, 674-679.
3. Ansorge, W., Sproat, B., Stegemann, J., Schwager, C., and Zenke, M. (1987) Nucleic Acids Res. 15, 4593-4602.
4. Wiemann, S., Stegemann, J., Grothues, D., Bosch, A., Estivill, X., Schwager, C., Zimmermann, J., Voss, H., and Ansorge, W. (1995) Anal. Biochem. 224, 117-121.
5. Henikoff, S. (1984) Gene 28, 351.
6. Voss, H., Wiemann, S., Grothues, D., Sensen, C., Zimmermann, J., Schwager, C., Stegemann, J., Erfle, H., Rupp, T., and Ansorge, W. (1993) BioTechniques 15, 714-721.
7. Ansorge, W., Voss, H., Wiemann, S., Schwager, C., Sproat, B., Zimmermann, J., Stegemann, J., Erfle, H., Hewitt, N., and Rupp, T. (1992) Electrophoresis 13, 616-619.
8. Wiemann, S., Rupp, T., Zimmermann, J., Voss, H., Schwager, C., and Ansorge, W. (1995) BioTechniques, 18, 688-697.
9. Voss, H., Wiemann, S., Wirkner, U., Schwager, C., Zimmermann, J., Stegemann, J., Erfle, H., Hewitt, N., Rupp, T., and Ansorge, W. (1992) Methods Mol. Cell. Biol. 3, 153-155.
10. Zimmermann, J., Voss, H., Schwager, C., Stegemann, J., Erfle, H., Stucky, K., Kristensen, T., and Ansorge, W. (1990) Nucleic Acids Res. 18, 1067.
11. Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughes, P., Dodd, C., Connel, C. R., Heiner, C., Kent, S. B. H., and Hood, L. E. (1986) Nature 321, 674-679.
12. Titus, J. A., Haugland, R., Sharrow, S. O., and Segal, D. M. (1982) J. Immunol. Methods 50, 193-204.
13. Tabor, S., and Richardson, C. C. (1989) J. Biol. Chem. 264, 6447-6458.
14. Bosch, A., Wiemann, S., Guimerà, J., Ansorge, W., Patterson, D., and Estivill, X. (1993) Hum. Mol. Genet. 2, 1744.
15. Bosch, A., Wiemann, S., Ansorge, W., Patterson, D., and Estivill, X. (1994) Hum. Genet. 93, 359-360.
16. Bosch, A., Wiemann, S., Guimerà, J., Ansorge, W., Patterson, D., and Estivill, X. (1995) Hum. Genet. 95, 119-122.
17. Church, G. M., and Kieffer-Higgins, S. (1988) Science 240, 185-188.
18. Cherry, J. L., Young, H., Di Sera, L. J., Ferguson, F. M., Kimball, A. W., Dunn, D. M., Gesteland, R. F., and Weiss, R. B. (1994) Genomics 20, 68-74.
19. Wiemann, S., Voss, H., Schwager, C., Rupp, T., Stegemann, J., Zimmermann, J., Grothues, D., Sensen, C., Erfle, H., Hewitt, N., Banrevi, A., and Ansorge, W. (1993) Yeast 9, 1343-1348.
20. Koop, B. F., Rowan, L., Chen, W.-Q., Deshpande, P., Lee, H., and Hood, L. (1993) BioTechniques 14, 442-447.
21. Voss, H., Schwager, C., Wiemann, S., Zimmermann, J., Stegemann, J., Erfle, H., Voie, A-M., Drzonek, H., and Ansorge, W. (1995) J. Biotechnol. 41, 121-129.
22. Roach, J., Boysen, C., Wang, K., and Hood, L. (1995) Genomics 26, 345-353.
23. Cripps, K. J., Purdie, C. A., Carder, P. J., White, S., Komine, K., Bird, C. C., and Wyllie, A. H. (1994) Oncogene 9, 2739-2743.
24. Frothingham, R., and Wilson, K. H. (1993) J. Bacteriol. 175, 2818-2825.
25. Tokita, H., Okamoto, H., Tsuda, F., Song, P., Nakata, S., Chosa, T., Iizuka, H., Mishiro, S., Miyakawa, Y., and Mayumi, M. (1994) Proc. Natl. Acad. Sci. USA 91, 11022-11026.
26. Versluis, L. F., Rozemuller, E.,Tonks, S., Marsh, S. G., Bouwens, A. G., Bodmer, J. G., and Tilanus, M. G. (1993) Hum. Immunol. 38, 277-283.