Pbotoaffinity labeling of elongation factor-2 with 8-azido derivatives of GTP and ATP

European Journal of Biochemistry

Volumn 236, Issue 1, 1996, Pages 149-154

  Guillot, D ^a   Vard, C ^a   Reboud, J P ^a  

^a CNRS   (France)

Author keywords

ATP; Elongation factor 2; GTP; Photoaffinity labeling

Indexed keywords

ADENOSINE TRIPHOSPHATE DERIVATIVE; ELONGATION FACTOR 2; GUANOSINE TRIPHOSPHATE DERIVATIVE; 8 AZIDOADENOSINE TRIPHOSPHATE; 8 AZIDOGUANOSINE TRIPHOSPHATE; 8-AZIDOADENOSINE 5'-TRIPHOSPHATE; 8-AZIDOGUANOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AZIDE; DRUG DERIVATIVE; ELONGATION FACTOR; GUANOSINE TRIPHOSPHATE; PEPTIDE FRAGMENT;

ANIMAL TISSUE; ARTICLE; BINDING SITE; CONTROLLED STUDY; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; ISOTOPE LABELING; LIVER; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; RAT; AFFINITY LABELING; AMINO ACID SEQUENCE; BINDING COMPETITION; CHEMISTRY; COMPARATIVE STUDY; LIGHT; METABOLISM; MOLECULAR GENETICS; RADIATION EXPOSURE;

ADENOSINE TRIPHOSPHATE; AFFINITY LABELS; AMINO ACID SEQUENCE; AZIDES; BINDING SITES; BINDING, COMPETITIVE; GUANOSINE TRIPHOSPHATE; LIGHT; MOLECULAR SEQUENCE DATA; PEPTIDE ELONGATION FACTOR 2; PEPTIDE ELONGATION FACTORS; PEPTIDE FRAGMENTS;

EID: 0029670026     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00149.x     Document Type: Article

References (20)

1. Aevarsson, A., Brazhnikov, E., Garber, M., Zheltonosova, J., Chirgadze, Y., Al‐Karadaghi, S., Svensson, L. A. & Liljas, A. (1994) Three‐dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus, EMBO J. 13, 3669–3677.
2. Conquet, F., Lavergne, J. P., Paleologue, A., Reboud, J. P. & Reboud, A. M. (1987) Partial reassembly of active 60S ribosomal subunits from rat liver following treatment with dimethylmaleic anhydride, Eur. J. Biochem. 163, 15–20.
3. Czworkowski, J., Wang, J., Steitz, T. A. & Moore, P. B. (1994) The crystal structure of elongation factor G complexed with GDP, at 2.7 Å resolution, EMBO J. 13, 3661–3668.
4. Geourjon, C., Deleage, G. & Roux, B. (1991) Antheprot: an interactive graphic software for analysing protein structures from sequences, J. Mol. Graphics 9, 188–190.
5. Guillot, D., Penin, F., Di Pietro, A., Sontag, B., Lavergne, J. P. & Reboud, J. P. (1993a) GTP‐binding to elongation factor eEF‐2 unmasks a tryptophan residue required for biological activity, J. Biol. Chem. 268, 20911–20916.
6. Guillot, D., Lavergne, J. P. & Reboud, J. P. (1993b) Trp221 is involved in the protective effect of elongation factor eEF‐2 on the ricin/α‐sarcin site of the ribosome, J. Biol. Chem. 268, 26082–26084.
7. Higgins, D. G. & Sharp, P. M. (1988) CLUSTAL: a package for performing multiple sequence alignements on a microcomputer, Gene (Amst.) 73, 237–244.
8. Hummel, J. P. & Dreyer, W. J. (1962) Measurement of protein‐binding phenomena by gel filtration, Biochim. Biophys. Acta 63, 530–532.
9. King, S. M., Kim, H. & Haley, B. H. (1991) Strategies and reagents for photoaffinity labeling of mechanochemical proteins, Methods Enzymol. 196, 449–466.
10. Kohno, K., Uchida, T., Ohkubo, H., Nakanishi, S., Nakanishi, T., Fukui, T., Ohtsuka, E., Ikehara, M. & Okada, Y. (1986) Amino acid sequence of mammalian elongation factor 2 deduced from the cDNA sequence: homology with GTP‐binding proteins, Proc. Natl Acad. Sci. USA 83, 4978–4982.
11. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227, 680–685.
12. Marzouki, A., Lavergne, J. P., Reboud, J. P. & Reboud, A. M. (1989) Heterogeneity of nature rat liver elongation factor 2, FEBS Lett. 255, 72–76.
13. Mimura, C. S., Admon, A., Hurt, K. A. & Ames, G. F. L. (1990) The nucleotide‐binding site of HisP, a membrane protein of the histidine permease, J. Biol. Chem. 265, 19535–19542.
14. Nilsson, L. & Nygård, O. (1985) Localization of the site of ADP‐ribosylation and GTP binding in the eukaryotic elongation factor EF‐2, Eur. J. Biochem. 148, 299–304.
15. Nilsson, L. & Nygård, O. (1988) Translationnal dynamics. Interactions between the translational factors, tRNA and ribosomes during eukaryotic protein synthesis, Eur. J. Biochem. 171, 293–299.
16. Olcott, M. C., Bradley, M. L. & Haley, B. H. (1994) Photoaffinity labeling of creatine kinase with 2‐azido and 8‐azidoadenosine triphosphate: identification of two peptides from the ATP‐binding domain, Biochemistry 33, 11935–11941.
17. Reboud, A. M., Dubost, S., Buisson, M. & Reboud, J. P. (1980) tRNA binding stabilizes rat liver 60S ribosomal subunits during treatment with LiCl, J. Biol. Chem. 255, 6954–6961.
18. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. & Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid, Anal. Biochem. 150, 76–85.
19. Sontag, B., Reboud, A. M., Divita, G., Di Pietro, A., Guillot, D. & Reboud, J. P. (1993) Intrinsic tryptophan fluorescence of rat liver elongation factor eEF‐2 to monitor the interaction with guanylic and adenylic nucleotides and related conformational changes. Biochemistry 32, 1976–1980.
20. Shoemaker, M., Lin, P. C. & Haley, B. (1992) Identification of the guanine binding domain peptide of the GTP‐binding site of glucagon, Protein Sci. 1, 884–891.