Evolution of scleractinian corals inferred from molecular systematics

Science

Volumn 271, Issue 5249, 1996, Pages 640-642

  Romano, Sandra L ^a,b   Palumbi, Stephen R ^a  

^a UNIVERSITY OF HAWAII   (United States)

^b DEPARTMENT OF PALEOBIOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CORAL; EVOLUTION; PHYLOGENY; SCLERACTINIAN; SYSTEMATICS;

EID: 0029668299     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5249.640     Document Type: Article

References (27)

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17. Genomic DNA extractions were from living, frozen, or ethanol-preserved colonies (8, 12). Highly enriched mtDNA was from the ProMega Wizard Minipreps system (8) [K. B Beckman, M F. Smith, C Orrego, Promega Notes 43, 10 (1993)]. Coral-specific primers (16Sd-L, 5′-GGTGAGACCTGCCCAATGGTT-3′, and 16Sc-H, 5′-AACAGCGCAATAACGTTTGAGAG-3′) amplified a 406- to 565-base pair (bp) segment (8), which was cloned and sequenced (8) with primers 16Sd-L, 16Sc-H, 16Se-H (5′-CGCCTTTAAAAAAGTAAC-3′), and 16Sf-L (5′-CTACATCCAAATTGTTAGAC-3′). Sequences have been deposited in GenBank (accession numbers L75992-L76024 and L76132) Replicate PCR sequences revealed most corals to be homoplasmic. However, three were heteroplasmic with infrequent amplifications of sequences from the opposite major clade. These results were repeatable from different DNA extractions and different colonies and were not found to result from contamination (8). The source of heteroplasmy of corals is unknown; hypotheses for the source include hybridization and unusual modes of mtDNA transmission (8)
18. Scleractinian sequences were aligned to each other and to sequences from a hydrozoan (13) and Renilla [D. Bridge, C W Cunningham, R. DeSalle, L W Buss, Mol. Biol Evol. 12, 679 (1995)], an anthozoan in the order Octocorallia This alignment (577 bp, of which 315 bp are variable and 210 bp are informative) includes 16 insertion-deletion events, which are not used in phylogenetic analyses, that map onto clades observed on the phylogenetic tree
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27. We thank S Cairns, J Maragos, and J. E. N Veron for assistance in understanding morphological characters of corals, and G Roderick for helpful suggestions Supported by grants from Sigma Xi, the Edmondson Fund, and the Constance Endicott Hartt Fund of the AAUW Honolulu Branch (to S L R) and from NSF (to S.R.P.)