Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst

Journal of Immunological Methods

Volumn 191, Issue 2, 1996, Pages 187-196

  Van Pelt, L J ^a   Van Zwieten, R ^a   Weening, R S ^a   Roos, D ^a,b   Verhoeven, A J ^a   Bolscher, B G J M ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b CENTRAL LABORATORY   (Netherlands)

Author keywords

Chronic granulomatous disease; Hydrogen peroxide; Myeloperoxidase; NADPH oxidase

Indexed keywords

DIHYDRORHODAMINE 123; MYELOPEROXIDASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; UNCLASSIFIED DRUG; AZIDE; CATALASE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; PEROXIDASE; RHODAMINE;

ARTICLE; CASE REPORT; CHRONIC GRANULOMATOUS DISEASE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; KINETICS; NEUTROPHIL; PRIORITY JOURNAL; RESPIRATORY BURST; CELL SEPARATION; CHEMISTRY; DRUG EFFECT; ENZYMOLOGY; FEMALE; IMMUNOLOGY; MALE; METABOLISM;

AZIDES; CATALASE; CELL SEPARATION; FEMALE; FLOW CYTOMETRY; HUMANS; INDICATORS AND REAGENTS; MALE; NEUTROPHILS; PEROXIDASE; RESPIRATORY BURST; RHODAMINES;

EID: 0029665620     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00024-5     Document Type: Article

References (32)

1. Akard, L.P., English, D. and Gabig, T.G. (1988) Rapid deactivation of NADPH oxidase in neutrophils: continuous replacement by newly activated enzyme sustains the respiratory burst. Blood 72, 322.
2. Bass, D.A., Parce, J.W., Dechatelet, L.R., Szejda, P., Seeds, M.C. and Thomas, M. (1983) Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J. Immunol. 130, 1910.
3. Becker, R. and Pfluger, K.H. (1994) Myeloperoxidase deficiency: an epidemiological study and flow-cytometric detection of other granular enzymes in myeloperoxidase-deficient subjects. Ann. Hematol. 69, 199.
4. Bolscher, B.G.J.M., Plat, H. and Wever, R. (1984) Some properties of eosinophil peroxidase, a comparison with other peroxidases. Biochim. Biophys. Acta 784, 177.
5. r 47 000, defective in autosomal chronic granulomatous disease, copurifies with one of two soluble components required for NADPH:O2 oxidoreductase activity in human neutrophils. J. Clin. Invest. 83, 757.
6. Bos, A.J., Weening, R.S., Hamers, M.N., Wever, R., Behrendt, H. and Roos, D. (1982) Characterization of hereditary partial myeloperoxidase deficiency. J. Lab. Clin. Med. 99, 589.
7. Bourgoin, S., Plante, E., Gaudry, M., Naccache, P.H., Borgeat, P. and Poubelle, P.E. (1990) Involvement of a phospholipase D in the mechanism of action of granulocyte-macrophage colony-stimulating factor (GM-CSF): priming of human neutrophils in vitro with GM-CSF is associated with accumulation of phosphatidic acid and diradylglycerol. J. Exp. Med. 172, 767.
8. Chanock, S.J., EI Benna, J., Smith, R.M. and Babior, B.M. (1994) The respiratory burst oxidase. J. Biol. Chem. 269, 24519.
9. Emmendörffer, A., Hecht, M., Lohmann Matthes, M.L. and Roesler, J. (1990) A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123. J. Immunol. Methods 131, 269.
10. Emmendörffer, A., Nakamura, M., Rothe, G., Spiekermann, K., Lohmann-Matthes, M.-L. and Roesler, J. (1995) Evaluation of flow cytometric methods for diagnosis of chronic granulomatous disease variants under routine laboratory conditions. Cytometry 18, 147.
11. English, D., Broxmeyer, H.E., Gabig, T.G., Akard, L.P., Williams. D.E. and Hoffman, R. (1988) Temporal adaptation of neutrophil oxidative responsiveness to N-formyl-methionyl-leucyl-phenylalanine. Acceleration by granulocyte-macrophage colony stimulating factor. J. Immunol. 141, 2400.
12. Jaswon, M.S., Khwaja, A., Roberts, P.J., Jones, H.M. and Linch, D.C. (1990) The effects of rhGM-CSF on the neutrophil respiratory burst when studied in whole blood. Br. J. Haematoi. 75, 181.
13. Klebanoff, S.J. (1988) Phagocytic cells: products of oxygen metabolism. In: J.I. Gallin, I.M. Goldstein and R. Snyderman (Eds.), Inflammation: basic principles and clinical correlates. Raven Press, New York, p. 391.
14. Koenderman, L., Yazdanbakhsh, M., Roos, D. and Verhoeven, A.J. (1989) Dual mechanisms in priming of the chemoattractant-induced respiratory burst in human granulocytes. A Ca2+ -dependent and a Ca2+ -independent route. J. Immunol. 142, 623.
15. Kuijpers, T.W., Koenderman, L., Weening, R.S., Verhoeven, A.J. and Roos, D. (1990) Continuous cell activation is necessary for stable interaction of complement receptor type 3 with its counter-structure in the aggregation response of human neutrophils. Eur. J. Immunol. 20, 501.
16. Kuijpers, T.W., Tool, A.T., Van der School, C.E., Ginsel, L.A., Underwater, J.J., Roos, D. and Verhoeven, A.J. (1991) Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood 78, 1105.
17. Lehrer, R.I. and Ganz, T. (1990) Antimicrobial polypeptides of human neutrophils. Blood 76, 2169.
18. Macey, M.G., Jiang, X.P., Veys, P., McCarthy, D. and Newland, A.C. (1992) Expression of functional antigens on neutrophils. Effects of preparation. J. Immunol. Methods. 149, 37.
19. Ohno, Y. and Gallin, J.I. (1985) Diffusion of extrtacetlular hydrogen peroxide into intracellular compartments of human neu-trophils. J. Biol. Chem. 260, 8438.
20. Clark, R.A., Volpp, B.D., Leidal, K.G., Nauseef, W.M. (1990) Two cytosolic components of the human neurtophil respiratory burst oxidase translocate to the plasma membrane curing cell activation. J. Clin. Invest. 85, 714.
21. Repo, H., Jansson, S.E. and Leirisalo Repo, M. (1993) Flow cytometric determination of CD11b upregulation in vivo. J. Immunol. Methods. 164, 193.
22. Roesler, J., Hecht, M., Freihorst, J., Lohmann Matthes, M.L. and Emmendörffer, A. (1991) Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry. Eur. J. Pediatr. 150, 161.
23. Roos, D, (1994) The genetic basis of chronic granulomatous disease. Immunol. Rev. 138, 121.
24. Roos, D., Voetman, A.A. and Meerhof, L.J. (1983) Functional activity of enucleated polymorphonuclear leukocytes. J. Cell Biol. 97, 368.
25. Root, R.K. and Metcalf, J.A. (1977) H2O2 release from human granulocytes during phagocytosis. Relationship to superoxide anion formation and cellular catabolism of H2O2: studies with normal and cytochalasin b-treated cells. J. Clin. Invest. 60, 1266.
26. Rothe, G. and Valet, G. (1990) Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′,7′-dichlorofluorescin. J. Leukoc. Biol. 47, 440.
27. Rothe, G. and Valet, G. (1994) Flow cytometric assays of oxidative burst activity in phagocytes. Methods Enzymol. 233, 539.
28. Rothe, G., Emmendörffer, A., Oser, A., Roesler, J. and Valet, G. (1991) Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123. J. Immunol. Methods 138, 133.
29. Smith, J.A. and Weidemann, M.J. (1993) Further characterization of the neutrophil oxidative burst by flow cytometry. J. Immunol. Methods 162, 261.
30. Smith, R.M. and Cumutte, J.T. (1991) Molecular basis of chronic granulomatous disease. Blood 77, 673.
31. Sullivan, R., Fredette, J.P., Socinski, M., Elias, A., Antman, K., Schnipper, L. and Griffin, J.D. (1989) Enhancement of superoxide an ion release by granulocytes harvested from patients receiving granulocyte-macrophage colony-stimulating factor. Br. J. Haematol. 71, 475.
32. Vowells, S.J., Sekhsaria, S., Malech, H.L., Shalit, M. and Fleisher, T.A. (1995) Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes. J. Immunol. Methods 178, 89.