Characterization of α-neurotoxin and phospholipase A2 activities from Micrurus venoms: Determination of the amino acid sequence and receptor-binding ability of the major α-neurotoxin from Micrurus nigrocinctus nigrocinctus

European Journal of Biochemistry

Volumn 238, Issue 1, 1996, Pages 231-239

  Rosso, Jean Pierre ^a   Vargas Rosso, Orietta ^a   Gutiérrez, José María ^b   Rochat, Hervé ^a   Bougis, Pierre E ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b UNIVERSIDAD DE COSTA RICA   (Costa Rica)

Author keywords

Micrurus; Phospholipase A2; Venom; neurotoxin

Indexed keywords

ANTHOZOA; ELAPIDAE; MARMORATA; MICRURUS; MICRURUS NIGROCINCTUS; SERPENTES; TORPEDO MARMORATA;

CHOLINERGIC RECEPTOR; NEUROTOXIN; PHOSPHOLIPASE A2; SNAKE VENOM; ALPHA NEUROTOXIN, MICRURUS; ALPHA-NEUROTOXIN, MICRURUS; AMINO ACID; PHOSPHOLIPASE A; TOXIN;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CHEMICAL ANALYSIS; CONTROLLED STUDY; DRUG PURIFICATION; ENZYME ASSAY; ENZYME PURIFICATION; FLUOROMETRY; GEOGRAPHIC DISTRIBUTION; NEUROTOXICITY; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; RECEPTOR BINDING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SNAKE; SPECIES DIFFERENCE; TORPEDO MARMORATA; ANIMAL; CHEMISTRY; COMPARATIVE STUDY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; MOLECULAR GENETICS; SEQUENCE HOMOLOGY; WESTERN HEMISPHERE;

AMERICAS; AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELAPID VENOMS; ELAPIDAE; MOLECULAR SEQUENCE DATA; NEUROTOXINS; PHOSPHOLIPASES A; PHYLOGENY; SEQUENCE HOMOLOGY, AMINO ACID; TOXINS, BIOLOGICAL;

EID: 0029665510     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0231q.x     Document Type: Article

References (47)

1. Alape-Girón, A., Lomonte, B., Gustafsson, B., Da Silva, N. J. & Thelestam, M. (1994) Electrophoretic and immunochemical studies of Micrurus snake venoms, Toxicon 32, 713-723.
2. 2 isolated from the venom of the coral snake Micrurus nigrocinctus nigrocinctus, Comp. Biochem. Physiol. 87B, 949-952.
3. Behrens, B. & Karber, C. (1935) Wie sind Reihenversuche für biologische Auswertungen am zweckmässigsten anzuordnen? Arch. Exp. Pathol. Pharmacol. 177, 379-388.
4. Bolanõs, R. & Cerdas, L. (1980) Producción y control de sueros antiofídicos en Costa Rica, Bol. Sanit. Pan-Am. 88, 189-196.
5. Bolanõs, R. (1972) Toxicity of costa rican snake venoms for the white mouse, Am. J. Trop. Med. Hyg. 21, 360-363.
6. Bolanõs, R., Cerdas, L. & Abalos, J. W. (1978) Venenos de las serpientes coral (Micrurus spp.): informe sobre un untiveneno polivalente para las américas, Bol. San. Pan. 84, 128-133.
7. Bolanõs, R., Piva, A., Taylor, R. & Flores, A. (1975) Natural resistance of bovine animals to Micrurus nigrocinctus renom, Toxicon 13, 369-370.
8. Bosc-Bierne, I., Rathelot, J., Canioni, P., Julien, R., Bechis, G., Gregoire, J., Rochat, H. & Sarda, L. (1981) Isolation and partial structural characterization of chicken pancreatic colipase, Biochem. Biophys. Acta 667, 225-232.
9. 2 activities, Biochemistry 25, 7235-7243.
10. Campbell, J. A. & Lamar, W. W. (1989) The venomous reptiles of Latin America, Comstock.
11. Changeux, J. P., Kasai, M. & Lee, C. Y. (1970) The use of a snake toxin to characterize the cholinergic receptor protein, Proc. Natl Acad. Sci. USA 67, 1241-1247.
12. Crestfeld, A., Moore, S. & Stein, W. (1963) The preparation and enzymic hydrolysis of reduced and S-carboxymethylated proteins, J. Biol. Chem. 238, 622.
13. Da Silva, N. J., Griffin, P. R. & Aird, S. D. (1991) Comparative chromatography of brasilian coral snakes (Micrurus) venoms. Comp. Biochem. Physiol. 100B, 117-126.
14. Dessen, P., Fondrat, C., Valencien, C. & Mugnier, C. (1990) BISANCE: a French service for access to biomolecular sequence databases, CABIOS 6, 355-356.
15. Dryer, S. E. & Chiappinelli, V. A. (1983) Kappa-bungarotoxin: an intracellular study demonstrating blockade of neuronal nicotinic receptors by a snake neurotoxin, Brain Res. 289, 317-321.
16. Goularte, F. C. L., Cruz-Hofling, M. A., Cogo, J. C., Gutiérrez, J. M. & Rodrigues-Simioni, L. (1995) The ability of specific antivenom and low temperature to inhibit the myotoxicity and neuromuscular block induced by Micrurus nigrocinctus venom, Toxicon 33, 679-689.
17. Gutiérrez, J. M., Arroyo, O., Chaves, F., Lomonte, B. & Cerdas, L. (1986) Pathogenesis of myonecrosis induced by coral snake (Micrurus nigrocinctus) venom in mice, Br. J. Exp. Path. 67, 1-12.
18. Gutiérrez, J. M., Chaves, F., Rojas, E. & Bolanõs, R. (1980) Effectos locales inducidos por el veneno de la serpiente coral Micrurus nigrocinctus en raton blanco, Toxicon 18, 633-639.
19. Gutiérrez, J. M., Lomonte, B., Portilla, E., Cerdas, L. & Rojas, E. (1983) Local effects induced by coral snake venoms: evidence of myonecrosis after experimental inoculations of venoms from five species, Toxicon 21, 777-784.
20. Gutiérrez, J. M., Rojas, G., Da Silva, N. J. & Nuñez, J. (1992) Experimental myonecrosis induced by the venoms of south american Micrurus (coral snakes), Toxicon 30, 1299-1302.
21. Haley, T. J. & Mc Cormick, W. G. (1957) Pharmalogical effects produced by intracerebral injection of drugs in the conscious mouse, Brit. J. Pharmacol. 12, 12-15
22. Harding, K. A. & Welch, K. R. G. (1980) Venonous snakes of the world: a checklist, Pergamon Press, England.
23. Joubert, F. & Taljaard, N. (1978) Purification, some properties and the primary structures of three reduced and S-carboxylated toxin (CM-5, CM-6 and Cm-10a) from Naja haje haje (Egyptian cobra) venom, Biochim. Biophys. Acta 537, 1-8.
24. Joubert, F. (1975) The amino sequences of three toxins (CM-10, CM-12 and CM-14) from Naja haje annulifera (Egyptian cobra) venom, Hoppe Seylers Z. Physiol. Chem. 356, 53-72.
25. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
26. Low, B., Preston, H. S., Sato, A., Rosen, L. S., Searl, J. E., Rudko, A. D. & Richardson, J. S. (1976) Three dimensional structure of erabutoxin b neurotoxic protein: Inhibitor of acetylcholine receptor, Proc. Natl Acad. Sci. USA 73, 2991-2994.
27. Marchot, P., Frachon, P. & Bougis, P. E. (1988) Selectivedistinction at equilibrium between the two α-neurotoxin binding sites of Torpedo acetylcholine receptor by microtitration, Eur. J. Biochem. 174, 537-542.
28. Martin, M. F., Rochat, H., Marchot, P. & Bougis, P. E. (1987) Use of high performance liquid chromatography to demonstrate quantitative variation in components of venom from the scorpion Androctonus australis Hector, Toxicon 25, 569-573.
29. Mc Dowell, S. B. (1987) in Systematics in snakes: ecology and evolutionary biology (Siegel, R. A., Collins, J. T. & Nowak, S. S., eds) pp. 3-50, Macmillan, New York.
30. Minton, S. A. (1979) in Snake venoms, handbook of experimental pharmacology (Lee, C. Y., ed.) vol. 52, pp. 845-862, Springer, Berlin.
31. Morel, N., Israel, M., Manaranche, R. & Mastour-Frachon, P. (1977) Isolation of pure cholinergic nerve endings from Torpedo electric-organ, Evaluation of their metabolic properties, J. Cell Biol. 75, 43-55.
32. Moussatche, H. & Melendez, T. (1979) Some pharmacological observations with Elapidae and Crotalidae snake venoms in the guinea-pig denervated diaphragm on the specificity of the cholinergic blockade by their venoms, Rev. Brasil. Biol. 39, 605-610.
33. Parmentier, J. & Carpenter, D. (1976) Blocking action of snake venom neurotoxins at receptor sites to putative central nervous system transmitters, in Animal, plant and microbial toxins (Ohsaka, A., Hayashi, K. & Sawai, Y., eds) vol. 2, pp. 179-191, Plenum Press, New-York and London.
34. Pillet, L., Trémeau, O., Ducancel, F., Drevet, P., Zin-Justin, S., Pinkasfeld, S., Boulain, J. C. & Ménez, A. (1993) Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis, J. Biol. Chem. 268, 909-916.
35. 2 from the venom of the coral snake, Micrurus fulvius microgalbineus, Biochem. J. 179, 603-606.
36. Ramsey, H. M., Snyder, G. K., Kitchen, H. & Taylor, W. S. (1971) Fractionation of coral snake venom. Preliminary studies on the separation and characterization of the protein fractions, Toxicon 10, 67-72.
37. Ramsey, H. M., Taylor, W. J., Boruchow, I. B. & Snyder G. K. (1972) Mechanism of shock produced by an elapid snake (Micrurus fulvius fulvius) venom in dogs. Am. J. Phys. 222, 782-786.
38. Reed, J. K. & Raftery, M. A. (1976) Properties of the tetrodotoxin binding component in plasma membranes isolated from Electrophorus electricus, Biochemistry 15, 944-953.
39. Riesfeld, R. A., Lewis, U. J. & Williams, D. E. (1962) Disc electrophoresis of basic proteins and peptides on polyacrylarnide gels, Nature 195, 281.
40. Rochat, H., Tessier, M., Miranda, F. & Lissitzky, S. (1977) Radioiodination of scorpion and snake toxins, Anal. Biochem. 82, 532-548.
41. Sato, S. & Tamiya, N. (1971) The amino acid sequences of erabutoxins, neurotoxic proteins of sea-snake (Laticauda semifasciata) venom, Biochem. J. 122, 453-461.
42. Swank, R. T. & Munkres, K. D. (1971) Molecular mass analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate, Anal. Biochem. 39, 462-477.
43. Tan, N. H. & Ponnudurai, G. (1992) The biological properties of venoms of some american coral snakes (genus Micrurus), Comp. Biochem. Physiol. 101B, 471-474.
44. Trémeau, O., Lemaire, C., Drevet, P., Pinkasfeld, S., Ducancel, F., Boulain, J. C. & Ménez, A. (1995) Genetic engineering of snake toxins. The functionnal site of erabutoxin a as delineated by site-directed mutagenesis includes variant residues, J. Biol. Chem. 270, 9362-9369.
45. Vital Brazil, O. & Fontana, M. D. (1983) Açoes pré-juncionas e posjuncionas da peçonha da cobra Micrurus corallinus na junçao neuromuscular, Mem. Inst. Butantan (Sao-Paulo) 47, 13-26.
46. Vital Brazil, O., Fontana, M. D. & Filho, A. P. (1976) Physiopathologie et thérapeutique de l'envenimation expérimentale causée par le venin de Micrurus fontalis, Mem. Inst. Butantan (Sao-Paulo) 40, 221-240.
47. Weis, R. & Mc Isaac, R. J. (1971) Cardiovascular and muscular effects of venom from coral snake Micrurus fulvius, Toxicon 9, 219-228.