p-Chlorophenylalanine changes serotonin transporter mRNA levels and expression of the gene product

Journal of Neurochemistry

Volumn 67, Issue 2, 1996, Pages 463-472

  Rattray, Marcus ^a   Baldessari, Sonia ^b,c   Gobbi, Marco ^b   Mennini, Tiziana ^b   Samanin, Rosario ^b   Bendotti, Caterina ^b  

^a UMDS   (United Kingdom)

^b MARIO NEGRI INSTITUTE FOR PHARMACOLOGICAL RESEARCH   (Italy)

^c UNIVERSITY OF MILAN   (Italy)

Author keywords

Autoradiography; Gene expression; Image analysis; In situ hybridization; p Chlorophenylalanine; Serotonin transporter; Serotonin uptake serotonin uptake sites; Tryptophan hydroxylase

Indexed keywords

CARRIER PROTEIN; CITALOPRAM; FENCLONINE; MESSENGER RNA; SEROTONIN; TRYPTOPHAN HYDROXYLASE; ENZYME INHIBITOR; MEMBRANE PROTEIN; NERVE PROTEIN; PRIMER DNA; SEROTONIN TRANSPORTER; SLC6A4 PROTEIN, RAT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DORSAL RAPHE NUCLEUS; ENZYME INHIBITION; GENE EXPRESSION; INTRAPERITONEAL DRUG ADMINISTRATION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR BINDING; RECEPTOR DOWN REGULATION; SEROTONIN RELEASE; SYNAPTOSOME; ANIMAL; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RAPHE NUCLEUS; SPRAGUE DAWLEY RAT;

ANIMALIA;

ANIMALS; BASE SEQUENCE; CARRIER PROTEINS; CITALOPRAM; DNA PRIMERS; ENZYME INHIBITORS; FENCLONINE; GENE EXPRESSION REGULATION; MEMBRANE GLYCOPROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; NERVE TISSUE PROTEINS; RAPHE NUCLEI; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; SEROTONIN; SEROTONIN PLASMA MEMBRANE TRANSPORT PROTEINS; SYNAPTOSOMES; TRYPTOPHAN HYDROXYLASE;

EID: 0029665451     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1996.67020463.x     Document Type: Article

References (52)

1. Åberg-Wigstedt A. (1989) The antidepressant effects of 5-HT uptake inhibitors. Br. J. Psychiatry 155 (Suppl. 8), 32-40.
2. Appel N. M., Contrera J. F., and De Souza E. B. (1989) Fenfluramine selectively and differentially decreases the density of serotonergic nerve terminals in rat brain: evidence from immunocytochemical studies. J. Pharmacol. Exp. Ther. 249, 928-943.
3. Appel N. M., Mitchell W. M., Contrera J. F., and De Souza E. B. (1990) Effects of high dose fenfluramine treatment on monoamine uptake sites in rat brain: assessment using quantitative autoradiography. Synapse 6, 33-44.
4. 3H]paroxetine-labeled serotonin uptake sites. J. Pharmacol Exp. Ther. 242, 911-916.
5. Battaglia G., Sharkey J., Kuhar M. J., and de Souza E. B. (1991) Neuroanatomic specificity and time course of alterations in rat brain serotonergic pathways induced by MDMA (3,4-methylenedioxymethamphetamine): assessment using quantitative autoradiography. Synapse 8, 249-290.
6. Bendotti C., Servadio A., Forloni G., Angeretti N., and Samanin R. (1990) Increased tryptophan hydroxylase mRNA in raphe serotoninergic neurons spared by 5,7-dihydroxytryptamine. Mol. Brain Res. 8, 343-348.
7. Bendotti C., Baldessari S., Ehret M., Tarizzo G., and Samanin R. (1993) Effect of d-fenfluramine and 5,7-dihydroxytryptamine on the levels of tryptophan hydroxylase and its mRNA in rat brain. Mol. Brain Res. 19, 257-261.
8. Blakely R. D., Berson H. E., Fremeau R. T. Jr., Caron M. G., Peck M. M., Prince H. K., and Bradley C. C. (1991) Cloning and expression of a functional serotonin transporter from rat brain. Nature 354, 66-70.
9. Boularand S., Darmon M. C., Ravassard P., and Mallet J. (1995) Characterization of the human tryptophan hydroxylase gene promoter. Transcriptional regulation by cAMP requires a new motif distinct from the cAMP-responsive element. J. Biol. Chem. 270, 3757-3764.
10. Brunswick D. J., Benmansour S., Tejani-Butt S. M., and Hauptmann M. (1992) Effects of high-dose methamphetamine on monoamine uptake sites in rat brain measured by quantitative autoradiography. Synapse 11, 287-293.
11. Burnet P. W. J., Michelson D., Smith M. A., Gold P. W., and Sternberg E. M. (1994) The effect of chronic imipramine administration on the densities of 5-HT1A and 5-HT2 receptors and the abundances of 5-HT receptor and transporter mRNA in the cortex, hippocampus and dorsal raphe of three strains of rat. Brain Res. 638, 311-324.
12. Chaput Y., Lesieur P., and de Montigny C. (1990) Effects of short-term, serotonin depletion on the the efficacy of serotonin neurotransmission: electrophysiological studies in the rat central nervous system. Synapse 6, 328-337.
13. Chen L., Hamaguchi K., Ogawa M., Hamada S., and Okada N. (1994) PCPA reduces both monaminergic afferents and nonmonaminergic synapses in the cerebral cortex. Neurosci. Res. 19, 111-115.
14. Cortés R., Mengod G., Celada P., and Artigas G. (1993) p-Chlorophenylalanine increases tryptophan-5-hydroxylase mRNA levels in the rat dorsal raphe: a time course study using in situ hybridization. J. Neurochem. 60, 761-764.
15. 3H]-imipramine. J. Pharmacol. Exp. Ther. 242, 364-371.
16. Darmon M. C., Guibert B., Leviel V., Ehret M., Maître M., and Mallet J. (1988) Sequence of two mRNAs encoding active rat tryptophan hydroxylase. J. Neurochem. 51, 312-316.
17. Delort J., Dumas J. B., Darmon M. C., and Mallet J. (1989) An efficient strategy for cloning 5′ extremities of rare transcripts permits isolation of multiple 5′-untranslated regions of rat tryptophan hydroxylase mRNA. Nucleic Acids Res. 17, 6439-6448.
18. 3H]Paroxetine binding and serotonin content of rat cortical areas, hippocampus, neostriatum, ventral mesencephalic tegmentum, and midbrain raphe nuclei following p-chlorophenylalanine and p-chloroamphetamine treatment. J. Neurochem. 58, 250-257.
19. Dumas S., Darmon M. C., Delort J., and Mallet J. (1989) Differential control of tryptophan hydroxylase expression in raphe and in pineal gland: evidence for a role of translation efficiency. J. Neurosci. Res. 24, 71-76.
20. Ehret M., Pevet P., and Maitre M. (1991) Tryptophan hydroxylase synthesis is induced by 3′,5′-cyclic adenosine monophosphate during circadian rhythm in the rat pineal gland. J. Neurochem. 57, 1516-1521.
21. Fujita M., Shimada S., Maeno H., Nishimura T., and Tohyama M. (1993) Cellular localisation of serotonin transporter messenger RNA in the rat brain. Neurosci. Lett. 162, 59-62.
22. Garattini S., Mennini T., Bendotti C., Invernizzi R., and Samanin R. (1986) Neurochemical mechanism of action of drugs which modify feeding via the serotoninergic system. Appetite 7, 15-38.
23. 3H] serotonin. Naunyn Schmiedebergs Arch. Pharmacol. 345, 1-6.
24. Gobbi M., Regondi M. C., Pompeiano M., Palacios J. M., and Mennini T. (1994) Differential effects of 5,7-dihydroxytryptamine-induced serotonergic degeneration on 5-HT1A receptors and 5-HT uptake sites in the rat brain. J. Chem. Neuroanat. 7, 65-73.
25. Gray E. G. and Whittaker V. P. (1962) The isolation of nerve endings from brain: an electron-microscope study of cell fragments derived by homogenization and centrifugation. J. Anat. 96, 79-87.
26. Hoffman B. J., Mezey E., and Brownstein M. J. (1991) Cloning of a serotonin transporter affected by antidepressants. Science 254, 579-580.
27. Hrdina P. D. and Vu T. B. (1993) Chronic fluoxetine treatment upregulates 5-HT uptake sites and 5-HT receptors in rat brain: an autoradiographic study. Synapse 14, 324-331.
28. Invernizzi R., Fracasso C., Caccia S., Di Clemente A., Garattini S., and Samanin R. (1989) Effect of L-cysteine on the long term depletion of brain indoles caused by p-chloroamphetamine and d-fenfluramine in rats. Relation to drug concentrations. Eur. J. Pharmacol. 163, 77-83.
29. Jéquier E., Lovenberg W., and Sjoerdsma A. (1967) Tryptophan hydroxylase inhibition: the mechanism by which p-chlorophenylalanine depletes rat brain serotonin. Mol. Pharmacol. 3, 274-278.
30. Kim K. S., Wessel T. C., Stone D. M., Carver C. H., Joh T. H., and Park D. H. (1991) Molecular cloning and characterisation of cDNA encoding tryptophan hydroxylase from rat central serotonergic system. Mol. Brain Res. 9, 277-283.
31. Koe B. K. and Weissman A. (1968) The pharmacology of parachlorophenylalanine, a selective depletor of serotonin stores, in Advances in Pharmacology, Vol. 6, Part B (Garattini S. and Shore P. A., eds), pp. 29-48. Academic Press, San Diego.
32. Kovachich G. B., Aronson C. E., and Brunswick D. J. (1992) Effect of repeated administration of antidepressants on serotonin uptake sites in limbic and neocortical structures of rat brain determined by quantitative autoradiography. Neuropsychopharmacology 7, 317-324.
33. Kuroda Y., Watanabe Y., and McEwen B. S. (1994) Tianeptine decreases both serotonin transporter messenger RNA and binding sites in rat brain. Eur. J. Pharmacol. Mol. Pharmacol. 268, R3-R5.
34. Lesch K. P., Aulakh C. S., Wolozin B. L., Tolliver T. J., Hill J. L., and Murphy D. L. (1993) Regional brain expression of serotonin transporter mRNA and its regulation by reuptake inhibiting antidepressants. Mol. Brain Res. 17, 31-35.
35. Linnet K., Koed K., Wiborg O., and Gregersen N. (1995) Serotonin depletion decreases serotonin transporter mRNA levels in rat brain. Brain Res. 697, 251-253.
36. Lopez J. F., Chalmers D. T., Vazquez D. M., Watson S. J., and Akil H. (1994) Serotonin transporter mRNA in rat brain is regulated by classical antidepressants. Biol. Psychiatry 35, 287-290.
37. Malter J. S. (1989) Identification of an AUUUA-specific messenger RNA binding protein. Science 246, 664-666.
38. Masseroli M., Bollea A., Bendotti C., and Forloni G. (1993) In situ hybridisation histochemistry quantification: automatic count on single cells in a digital image. J. Neurosci. Methods 47, 93-103.
39. Mennini T., Garattini S., and Caccia S. (1985) Anorectic effect of fenfluramine isomers and metabolites: relationship between brain levels and in vitro potencies on serotonergic mechanisms. Psychopharmacology (Berlin) 85, 111-114.
40. Miller F. P., Cox R. H. Jr., Snodgrass W. R., and Maickel R. P. (1970) Comparative effects of p-chlorophenylalanine, p-chloroamphetamine and p-chloro-N-methylamphelamine on rat brain norepinephrine, serotonin and 5-hydroxyindole-3 acetic acid. Biochem. Pharmacol. 19, 435-442.
41. Munson P. J. and Rodbard D. (1984) Computerized analysis of ligand binding data: basic principles and recent development, in Computers in Endocrinology (Rodbard D. and Forti G., eds), pp. 117-145. Raven Press, New York.
42. Park D. H., Stone D. M., Baker H., Kim K. S., and Joh T. H. (1994) Early induction of rat brain tryptophan hydroxylase (TPH) mRNA following parachlorophenylalanine (PCPA) treatment. Mol. Brain Res. 22, 20-28.
43. Paxinos G. and Watson C. (1982) The Rat Brain in Stereotaxic Coordinates. Academic Press, Sydney.
44. Rattray M., Wotherspoon G., Savery D., Marden C., Lopez Costa J. J., Bendotti C., and Priestley J. V. (1993) Fenfluramine and MDMA down-regulate serotonin transporter messenger RNA in subdivisions of the rat dorsal raphe complex. Soc. Neurosci. Abstr. 19, 495.
45. Rattray M., Wotherspoon G., Savery D., Baldessari S., Marden C., Priestley J. V., and Bendotti C. (1994) Chronic d-fenfluramine decreases serotonin transporter messenger RNA expression in dorsal raphe nucleus. Eur. J. Pharmacol. Mol. Pharmacol. 268, 439-442.
46. Richard F., Sanne J. L., Bourde O., Weissmann D., Ehret M., Cash C., Maitre M., and Pujol J. F. (1990) Variation of tryptophan-5-hydroxylase concentration in the rat raphe dorsalis nucleus after p-chlorophenylalanine administration. I. A model to study the turnover of the enzymatic protein. Brain Res. 536, 41-45.
47. Rudnick G. and Wall S. C. (1992) The molecular mechanism of "ecstasy" 3,4-methylenedioxymethamphetamine (MDMA): serotonin transporters are targets for MDMA-induced serotonin release. Proc. Natl. Acad. Sci. USA 89, 1817-1821.
48. Schuster C. R., Lewis M., and Seiden L. S. (1986) Fenfluramine: neurotoxicity. Psychopharmacol. Bull. 22, 148-151.
49. Spurlock G., Buckland P., O'Donovan M., and McGuffin P. (1994) Lack of effect of antidepressant drugs on the levels of mRNAs encoding serotonergic receptors, synthetic enzymes and 5HT transporter. Neuropharmacology 33, 433-440.
50. Tohyama I., Kameyama M., and Kimura H. (1988) Quantitative morphometric analysis of two types of serotonin-immunoreactive nerve fibres differentially responsive to p-chlorphenylalanine treatment in the rat brain. Neuroscience 26, 971-991.
51. Weissmann D., Chamba G., Debure L., Rousset C., Richard F., Maitre M., and Pujol J. F. (1990) Variation of tryptophan 5-hydroxylase concentrations in the rat dorsalis nucleus after p-chlorophenylalanine administration. II. Anatomical distribution of the tryptophan-5-hydroxylase protein and regional variation of its turnover rate. Brain Res. 536, 46-55.
52. Zaczek R., Battaglia G., Culp S., Appel N. M., Contrera J. F., and De Souza E. B. (1990) Effects of repeated fenfluramine administration on indices of monoamine function in rat brain: pharmacokinetic, dose response, regional specificity and time course data. J. Pharmacol. Exp. Ther. 253, 104-112.