Extracellular PagC-HlyA(s) fusion protein for the generation and identification of Salmonella specific antibodies

Applied Microbiology and Biotechnology

Volumn 45, Issue 5, 1996, Pages 629-637

  Mollenkopf, H J ^a   Gentschev, I ^a   Bubert, A ^a   Schubert, P ^a   Goebel, W ^b  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b MERCK KGAA   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; MONOCLONAL ANTIBODY; OUTER MEMBRANE PROTEIN; SYNTHETIC PEPTIDE; BACTERIAL PROTEIN; BACTERIUM ANTIBODY; ESCHERICHIA COLI PROTEIN; HEMOLYSIN; HLYA PROTEIN, E COLI; MEMBRANE PROTEIN; PAGC PROTEIN, SALMONELLA TYPHIMURIUM;

ANTIBODY PRODUCTION; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; HUMORAL IMMUNITY; IMMUNOBLOTTING; MOLECULAR CLONING; NONHUMAN; PROTEIN DOMAIN; RABBIT; SALMONELLA TYPHIMURIUM; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; BLOOD; GENETICS; GRAM NEGATIVE BACTERIUM; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SALMONELLA; SALMONELLOSIS; SPECIES DIFFERENCE;

ESCHERICHIA COLI; NEGIBACTERIA; ORYCTOLAGUS CUNICULUS; SALMONELLA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, BACTERIAL; ANTIBODIES, MONOCLONAL; BACTERIAL PROTEINS; BASE SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GRAM-NEGATIVE BACTERIA; HEMOLYSIN PROTEINS; HUMANS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; RABBITS; RECOMBINANT FUSION PROTEINS; SALMONELLA; SALMONELLA INFECTIONS; SALMONELLA TYPHIMURIUM; SPECIES SPECIFICITY;

EID: 0029665415     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050740     Document Type: Article

References (35)

1. Alpuche Aranda CM, Swanson JA, Loomis WP, Miller SI (1992) Salmonella typhimurium activates virulence gene transcription within acidified macrophage phagosomes. Proc Natl Acad Sci USA 89:10079-10083
2. Barondess JJ, Beckwith J (1990) A bacterial virulence determinant encodes by lysogenic coliphage 2. Nature 346:871-874
3. Gentschev I, Hess J, Goebel W (1990) Change in the cellular localization of alkaline phosphatase by alteration of its carboxy-terminal sequence. Mol Gen Genet 222:211-216
4. Gentschev I, Sokolovic Z, Köhler S, Krone GF, Hof H, Wagner J, Goebel W (1992) Identification of p60 antibodies in human sera and presentation of this Listerial antigen on the surface of attenuated salmonellae by the HlyB-HlyD secretion system. Infect Immun 60:5091-5098
5. Goebel W, Hedgpeth J (1982) Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli. J Bacteriol 151:1290-1298
6. Härtlein M, Schießl S, Wagner W, Rdest U, Kreft J, Goebel W (1983) Transport of hemolysin by Escherichia coli. J Cell Biochem 22:87-97
7. Heffernan EJ, Harwood J, Fierer J, Guiney D (1992) The Salmonella typhimurium virulence plasmid complement resistance gene rck is homologous to a family of virulence-related outer membrane protein genes, including pagC and ail. J. Bacteriol 174:84-91
8. Hess J, Gentschev I, Goebel W, Jarchau T (1990) Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins. Mol Gen Genet 224:201-208
9. Jacobs E, Clad A (1986) Electroelution of fixed and stained membrane proteins from preparative SDS-polyacrylamide gels into a membrane trap. Anal Biochem 154:583-589
10. Jarchau T, Chakraborty T, Garcia F, Goebel W (1994) Selection for transport competence of C-terminal polypeptides from Escherichia coli hemolysin: the shortest peptide capable of autonomous HlyB/HlyD-dependent secretion comprises the 62 C-terminal amino acids of HlyA. Mol Gen Genet 245:53-60
11. Kearny JF (1984) Hybridomas and monoclonal antibodies. Paul WE (ed) Fundamental immunology. Raven Press, New York, pp 751-756
12. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105-132
13. Koronakis V, Koronakis E, Hughes C (1989) Isolation and analysis of the C-terminal signal directing the export of Escherichia coli haemolysin protein across both bacterial membranes. EMBO J 8:595-605
14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
15. Ludwig A, Vogel M, Goebel W (1987) Mutations affecting activity and transport of hemolysin in Escherichia coli. Mol Gen Genet 206:238-245
16. Mackman N, Baker K, Gray L, Haigh R, Nicaud J-M, Holland IB (1987) Release of a chimeric protein into the medium from Escherichia coli using the C-terminal signal of hemolysin. EMBO J 6:2835-2841
17. Miller JF, Mekalanos JJ, Falkow S (1989) Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 243:916-922
18. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
19. Miller SI (1991) PhoP/PhoQ: macrophage-specific modulators of Samonella virulence. Mol Microbiol 5:2073-2078
20. Miller SI, Kukral AM, Mekalanos JJ (1989) A two-component regulatory system (phoP-phoQ) controls Salmonella typhimurium virulence. Proc Natl Acad Sci USA 86:5054-5058
21. Miller VL, Bliska JB, Falkow S (1990) Nucleotide sequence of the Yersinia enterocolitica ail gene and characterization of the Ail protein product. J Bacteriol 172:1062-1069
22. Miller VL, Beer KB, Loomis WP, Olson JA, Miller SI (1992) An unusual payC::TnphoA mutation leads to an invasion- and virulence-defective phenotype in salmonellae. Infect Immun 60:3763-3770
23. Mollenkopf, H-J (1995) PhD thesis. University of Würzburg, Germany
24. Neu HC, Heppel LA (1965) The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Bacteriol 240:3685-3692
25. Pulkkinen WS, Miller SI (1991) A Salmonella typhimurium virulence protein is similar to a Yersinia enterocolitica invasion protein and a bacteriophage lambda outer membrane protein. J Bacteriol 173:86-93
26. Sanger F, Nicklen S, Coulsen AR (1977) DNA sequencing with chain-termination inhibitors. Proc Natl Acad Sci USA 74:5463-5467
27. Stoorvogel J, Bussel MJAWM van, Tommassen J, Klundert JAM van de (1987) Cloning of a betalactam resistance determinant of Enterobacter cloacae affecting outer membrane proteins of Enterobacteriaceae. FEMS Microbiol Lett 48:277-281
28. Stoorvogel J, Bussel MJAWM van, Tommassen J, Klundert JAM van de (1991) Molecular characterization of an Enterobacter cloacae outer membrane protein (OmpX). J Bacteriol 173: 156-160
29. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354
30. Van Regenmortel MHV, Briand JP, Muller S, Plaue S (1990) Laboratory techniques in biochemistry and molecular biology: synthetic polypeptides as antigens, vol 19. Eisevier, New York
31. Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with universal primers. Gene 19:259-268
32. Vogel M, Hess J, Then I, Juarez A, Goebel W (1988) Characterization of a sequence (hlyR) which enhances synthesis and secretion of hemolysin in Escherichia coli. Mol Gen Genet 212:76-84
33. Wagner W, Vogel M, Goebel W (1983) Transport of hemolysin across the outer membrane of 'Escherichia coli requires two functions. J Bacteriol 154:200-210
34. Wandersman C, Delepelaire P (1990) TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. Proc Natl Acad Sci USA 87:4776-4780
35. Weig M (1994) PhD thesis. University of Würzburg, Germany