Characterization of the P2′ and P3′ specificities of thrombin using fluorescence-quenched substrates and mapping of the subsites by mutagenesis

Biochemistry

Volumn 35, Issue 22, 1996, Pages 7114-7122

  Le Bonniec, Bernard F ^a   Myles, Timothy ^a   Johnson, Tony ^c   Knight, C Graham ^b   Tapparelli, Carlo ^d   Stone, Stuart R ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c Peptide Therapeutics Ltd   (United Kingdom)

^d NONE

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME INHIBITOR; LYSINE; PEPTIDE; PHENYLALANINE; RECOMBINANT PROTEIN; THROMBIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BINDING SITE; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; FLUORESCENCE; GENETICS; HUMAN; HYDROLYSIS; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; RABBIT; SITE DIRECTED MUTAGENESIS; SYNTHESIS; AMINO ACID SUBSTITUTION; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CATTLE; ENZYME INHIBITORS; FLUORESCENCE; HUMANS; HYDROLYSIS; KINETICS; LYSINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDES; PHENYLALANINE; RABBITS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; THROMBIN;

EID: 0029665226     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi952701s     Document Type: Article

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