Analysis of the specificity of the AMP-activated protein kinase by site-directed mutagenesis of bacterially expressed 3-hydroxy 3-methylglutaryl-CoA reductase, using a single primer variant of the unique-site-elimination method

European Journal of Biochemistry

Volumn 237, Issue 3, 1996, Pages 800-808

  Ching, Yick Pang ^a   Davies, Stephen P ^a   Hardie, D Grahame ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

AMP activated protein kinase; Hydroxymethylglutaryl CoA reductase; Protein kinase specificity; Site directed mutagenesis; Unique site elimination

Indexed keywords

CRICETINAE; CRICETULUS GRISEUS; ESCHERICHIA COLI;

CYCLIC AMP DEPENDENT PROTEIN KINASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; 5' AMP ACTIVATED PROTEIN KINASE; 5'-AMP-ACTIVATED PROTEIN KINASE; MULTIENZYME COMPLEX; PRIMER DNA; PROTEIN KINASE; PROTEIN SERINE THREONINE KINASE; RECOMBINANT PROTEIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GENE EXPRESSION; HAMSTER; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; ANIMAL; BINDING SITE; CATALYSIS; CHO CELL; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; POINT MUTATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BINDING SITES; CATALYSIS; CHO CELLS; CRICETINAE; DNA PRIMERS; ESCHERICHIA COLI; GENETIC VECTORS; HYDROXYMETHYLGLUTARYL COA REDUCTASES; KINETICS; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; POINT MUTATION; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 0029665214     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0800p.x     Document Type: Article

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