Purification of a 3β-hydroxy-Δ5-C27-steroid dehydrogenase from pig liver microsomes active in major and alternative pathways of bile acid biosynthesis

Journal of Biological Chemistry

Volumn 271, Issue 34, 1996, Pages 20903-20907

  Purster, Catrin ^a   Zhang, Jie ^b   Toll, Anders ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

3BETA HYDROXY DELTA5 STEROID DEHYDROGENASE; BILE ACID; 3 BETA HYDROXY DELTA 5 C(27) STEROID DEHYDROGENASE; 3 BETA-HYDROXY-DELTA 5-C(27)-STEROID DEHYDROGENASE; 3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; CHOLESTEROL DERIVATIVE; MONOCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BILE ACID SYNTHESIS; BIOSYNTHESIS; CONTROLLED STUDY; ENZYME PURIFICATION; ENZYME SUBSTRATE; ISOELECTRIC FOCUSING; LIVER MICROSOME; MALE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SWINE; ANIMAL; BAGG ALBINO MOUSE; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; MOUSE; PH; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

3-HYDROXYSTEROID DEHYDROGENASES; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BILE ACIDS AND SALTS; HYDROGEN-ION CONCENTRATION; HYDROXYCHOLESTEROLS; MICE; MICE, INBRED BALB C; MICROSOMES, LIVER; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; SWINE;

EID: 0029664964     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.34.20903     Document Type: Article

References (25)

1. Björkhem, I. (1985) in New Comprehensive Biochemistry (Danietsson, H. and Sjövall, J., eds) Vol. 12, pp. 231-278, Eisevier Science Publishing Co., Amsterdam
2. Björkhem, I. (1969) Kur. J. Biochem. 8, 337-344
3. Wikvall, K. (1981) J. Biol. Chem. 256, 3376-3380
4. Mitropoulos, K. A., and Myant, N. B. (1967) Biochem. J. 103, 472-479
5. Mitropoulos, K. A., Avery, M. D., Myant, N. B., and Gibbons, G. F. (1972) Biochem. J. 130, 363-371
6. Javitt, N. B., Kok, E., Carubbi, F, Blizzard, T., Gut, M., and Byon, C. Y. (1986) J. Biol. Chem. 281, 12486-12489
7. Axelson, M., Shoda, J., Sjövall, J., Toll, A., and Wikvall, K. (1992) J. Biol. Chem. 267, 1701-1704
8. Shoda, J., Toll, A., Axelson, M., Pieper, F., Wikvall, K., and Sjövall, J. (1993) Hepatology 17, 395-403
9. Toll, A., Shoda, J., Axelson, M., Sjövall, J, and Wikvall, K. (1992) FEBS Lett. 298, 73-76
10. Shoda, J., Axelsson, M., and Sjövall, J. (1993) Steroids 58, 119-125
11. Andersson, S., Boström, H., Danielsson, H., and Wikvall, K. (1985) Methods Enzymol. 111, 364-377
12. Cheetham, P. S. J. (1979) Anal. Biochem. 92, 447-452
13. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. (1985) Anal. Biochem. 150, 76-85
14. Brown, R. E., Jarvis, K. L., and Hyland, K. J. (1989) Anal. Biochem. 180, 136-139
15. Andersson, S., and Jörnvall, H. (1986) J. Biol. Chem. 261, 16932-16936
16. Björkhem, J., Danielsson, H., and Wikvall, K. (1973) Eur. J. Biochem. 36, 8-15
17. Laemmli, U. K. (1970) Nature 227, 680-685
18. Wray, W., Boulikas, T., Wray, V. R, and Hancock, R. (1981) Anal. Biochem. 118, 197-203
19. Towbin, H., Staehelin, T., and Gordon, J. (1979) Proc. Nat. Acad. Sci. U. S. A. 76, 4350-4354
20. Matsudaira, P. (1987) J. Biol. Chem. 262, 10035-10038
21. Moos, M., Jr., Nguyen, N. Y., and Liu, T.-Y. (1987) J. Biol. Chem. 263, 6005-6008
22. Warner, M., Ahlgren, R., Zaphiropoulos, P. G., Hayashi, S.-I., and Gustafsson, J.-Å. (1991) Methods Enzymol. 206, 631-640
23. Labrie, F., Simard, J., Luu-The, V., Bélanger, A., and Pelletier, G. (1992) J. Steroid Biochem. Mol. Biol. 43, 805-826
24. Ichimiya, H., Egestad, B., Nazer, H., Baginski, E. S., Clayton, P. T., and Sjövall, J. (1991) J. Lipid Res. 32, 829-841
25. Buchmann, M. S., Kvittingen, E. A., Nazer, H., Gunasekaran, T., Clayton, P. T., Sjövall, J., and Björkhem, I. (1990) J. Clin. Invest. 86, 2034-2037