Yeast Nle3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex

Molecular and Cellular Biology

Volumn 16, Issue 5, 1996, Pages 2025-2036

  Kenna, Margaret A ^a   Petranka, John G ^a   Reilly, Jennifer L ^a   Davis, Laura I ^a,b  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS RECEPTOR; NUCLEOPORIN; FUNGAL PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; NUCLEAR PROTEIN; NUP170 PROTEIN, S CEREVISIAE; PRIMER DNA; SACCHAROMYCES CEREVISIAE PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL NUCLEUS; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STOICHIOMETRY; AMINO ACID SEQUENCE; BIOSYNTHESIS; CELL NUCLEUS MEMBRANE; COMPARATIVE STUDY; GENETIC COMPLEMENTATION; GENETICS; GENOTYPE; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PHENOTYPE;

MAMMALIA; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA PRIMERS; FUNGAL PROTEINS; GENETIC COMPLEMENTATION TEST; GENOTYPE; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NUCLEAR ENVELOPE; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; PHENOTYPE; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0029664656     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/mcb.16.5.2025     Document Type: Article

References (69)

1. Aitchison, J. D., M. P. Rout, M. Marelli, G. Blobel, and R. W. Wozniak. 1995. Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the venebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J. Cell Biol. 131: 1133-1148.
2. Akey, C. W., and D. S. Goldfarb. 1989. Protein import through the nuclear pore complex is a multi-step process. J. Cell Biol. 109:971-982.
3. Altschul, S. F., M. S. Boguski, W. Gish, and J. C. Wootton. 1994. Issues in searching molecular sequence databases. Nat Genet 6:119-129.
4. Baudin, A., O. Ozier-Kalogeropoulos, A. Denouel, F. Lacroute, and C. Cullin. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21:3329-3330.
5. Beddow, A. L., S. A. Richards, N. R. Orem, and I. G. Macara. 1995. The RanTC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif Proc. Natl. Acad. Sci. USA 92:3328-3332.
6. Belanger, K. D., and L. I. Davis. Unpublished data.
7. Belanger, K. D., M. A. Kenna, S. Wei, and L. I. Davis. 1994. Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p. J. Cell Biol. 126:619-630.
8. Bischoff, F. R., H. Krebber, T. Kempf, I. Hermes, and H. Ponstingl. 1995 Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Natl. Acad Sci USA 92:1749-1753.
9. Bischoff, F. R., H. Krebber, E. Smirnova, W. Dong, and H. Ponstingl. 1995. Co-activation of RanGTPase and inhibition of GTP dissociation by RanGTP binding protein RanBP1. EMBO J. 14:705-715.
10. Boeke, J. D., F. LaCroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen Genet. 197:345-346
11. Bogerd, A. M., J. A. Hoffman, D. C. Amberg, G. R. Fink, and L. I. Davis. 1994. nup1 mutants exhibit pleiotropic defects in nuclear pore complex function. J. Cell Biol. 127:319-332.
12. Bogerd, H. P., R. A. Fridell, S. Madore, and B. R. Cullen. 1995. Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins. Cell 82:485-494.
13. Butler, G., and K. H. Wolfe. 1994. Yeast homologue of mammalian Ran binding protein 1. Biochim. Biophys. Acta 1219:711-712.
14. Byrd, D. A., D. J. Sweet, N. Pante, K. N. Konstantinov, T. Guan, A. C. S. Saphire, P. J. Mitchell, C. S. Cooper, U. Aebi, and L. Gerace. 1994. Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex. J. Cell Biol. 127:1515-1526.
15. Cordes, V. C., S. Reidenhach, A. Kohler, N. Stuurman, R. van Driel, and W. W. Franke. 1993. Intranuclear filaments containing a nuclear pore complex protein J. Cell Biol. 123:1333-1344.
16. Davis, L. I. 1995. The nuclear pore complex. Annu. Rev. Biochem. 64: 865-896.
17. Davis, L. I., and G. Blobel. 1986. Identification and characterization of a nuclear pore complex protein. Cell 45:699-709.
18. Davis, L. I., and G. R. Fink. 1990 The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61:965-978.
19. Dingwall, C., S. Kandels-Lewis, and B. Seraphin. 1995 A family of Ran binding proteins that includes nucleoporins. Proc. Natl. Acad. Sci. USA 92: 7525-7529
20. Evan, G. I., G. K. Lewis, G. Ramsay, and J. M. Bishop. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product Mol. Cell. Biol. 5:3610-3616.
21. Fabre, E., W. C. Boelens, C. Wimmer, I. W. Mattaj, and E. C. Hurt. 1994. Nup145p is required for nuclear export of mRNA and binds humopolymeric RNA in vitro via a novel conserved motif. Cell 76:275-289.
22. Featherstone, C., M. K. Darby, and L. Gerace. 1988. A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo. J. Cell Biol. 107:1289-1297.
23. Feinberg, A. P., and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
24. Gorsch, L. C., T. C. Dockendorff, and C. N. Cole. 1995. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J. Cell Biol. 129:939-955
25. Grandi, P., V. Doye, and E. C. Hurt. 1993. Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J. 12:3061-3071.
26. Grandi, P., S. Emig, C. Weise, F. Hucho, T. Pohl, and E. C. Hurt. 1995. A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J Cell Biol 130:1263-1273.
27. Grandi, P., N. Schlaich, H. Tekotte, and E. C. Hurt. 1995. Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p. Nup49p and a novel protein Nup57p. EMBO J. 14:76-87.
28. + RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene J. Cell Biol. 131:1677-1697.
29. Hoffman, J. A., and L. I. Davis. Unpublished data.
30. + RNA export J. Cell Biol. 130:1275-1281
31. Iovine, M. K., J. L. Watkins, and S. R. Wente. 1995. The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor. J. Cell Biol. 131:1699-1713
32. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
33. Kraemer, D., R. W. Wozniak, G. Blobel, and A. Radu. 1994. The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm. Proc Natl Acad. Sci. USA 91:1519-1523.
34. Lee, L., and L. I. Davis. Unpublished data.
35. + RNA and constitutive clustering of nuclear pore complexes Mol. Biol Cell 6:401-417
36. Loeb, J. D. J., L. Davis, and G. F. Fink. 1993. NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex. Mol. Biol. Cell 4:209-222
37. Mehlin, H., B. Daneholt, and U. Skoglund. 1992. Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography. Cell 69:605-613.
38. Melchior, F., and L. Gerace. 1995. Mechanisms of nuclear protein import. Curr. Opin. Cell Biol. 7:310-318
39. Melchior, F., T. Guan, N. Yokojama, T. Nishimoto, and L. Gerace. 1995. GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import. J. Cell Biol 131:571-581.
40. Melchior, F., K. Weber, and V. Gerke. 1993. A functional homologue of the RNA1 gene product in Schizosaccharomyces pombe: purification, biochemical characterization, and identification of a leucine-rich repeat motif Mol. Biol. Cell 4:569-581.
41. Moore, M. S., and G. Blobel. 1993 The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature (London) 365:661-663.
42. Mutvei, A., S. Dihlmann, W. Berth, and E. C. Hurt. 1992. NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation. Eur. J. Cell Biol. 59:280-295.
43. Nehrbass, U., E. Fabre, S. Dihlmann, W. Herth, and E. C. Hurt. 1993. Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of nuclear pore protein NSP1. Eur. J. Cell Biol. 62:1-12.
44. Nehrbass, U., H. Kern, A. Mutvei, H. Horstmann, B. Marshallsay, and E. Hurt. 1990. NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell 61: 979-989.
45. Ouspenski, I. I., U. W. Mueller, A. Matynia, S. Sazer, S. J. Elledge, and B. R. Brinkley. 1995. Ran-binding protein-1 is an essential component of the Ran/RCC1 molecular switch system in budding yeast. J. Biol. Chem. 270: 1975-1978.
46. Pante, N., R. Bastos, I. McMorrow, B. Burke, and U. Aebi. 1994. Interactions and three-dimensional localization of a group of nuclear pore complex proteins. J. Cell Biol. 126:603-617.
47. Pemberton, L. F., M. P. Rout, and G. Blond. 1995. Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes. Proc. Natl. Acad. Sci. USA 92:1187-1191.
48. Powers, M. A., and D. J. Forbes. 1994. Cytosolic factors in nuclear transport: what's importin? Cell 79:931-934.
49. Radu, A., G. Blobel, and R. W. Wozniak. 1993. Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA. J. Cell Biol. 121:1-9.
50. Radu, A., M. S. Moore, and G. Blobel. 1995. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell 81:215-222.
51. Rexach, M., and G. Blobel. 1995. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83:683-692.
52. Richards, S. A., K. A. Lounsbury, and I. G. Macara. 1995. The C terminus of the nuclear RAN/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1, RAN-GAP, and HTF9A/RANBP1. J. Biol. Chem. 270:14405-14411.
53. Richardson, W. D., A. D. Mills, S. M. Dilworth, R. A. Laskey, and C. Dingwall. 1988 Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through the nuclear pores. Cell 52:655-664.
54. Rout, M. P., and G. Blobel. 1993. Isolation of the yeast nuclear pore complex. J. Cell Biol. 123:771-783.
55. Rout, M. P., and S. R. Wente. 1994. Pores for thought: nuclear pore complex proteins. Trends Cell Biol. 4:357-365.
56. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
57. Schlenstedt, G., E. Hurt, V. Doye, and P. A. Silver. 1993. Reconstitution of nuclear protein transport with semi-intact yeast cells. J. Cell Biol. 123:785-798.
58. Schlenstedt, G., D. H. Wong, D. M. Koepp, and P. A. Silver. 1995. Mutants in a yeast Ran binding protein are defective in nuclear transport. EMBO J. 14:5367-5378.
59. Sherman, F., G. R. Fink, and J. B. Hicks. 1986. Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
60. Snow, C. M., A. Senior, and L. Gerace. 1987. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J. Cell Biol. 104:1143-1156.
61. Vallette, F., E. Merge, A. Reiss, and M. Adesnik. 1989. Construction of mutant and chimeric genes using the polymerase chain reaction. Nucleic Acids Res. 17:723-733.
62. Wente, S. R., and G. Blobel. 1993. A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic. J Cell Biol. 123:275-284.
63. Wente, S. R., and G. Blobel. 1994. NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J. Cell Biol. 123:955-969.
64. Wente, S. R., M. R. Rout, and G. Blobel. 1992. A new family of yeast nuclear pore complex proteins. J Cell Biol. 119:705-723.
65. Wozniak, R. W., E. Bartnik, and G. Blobel. 1989. Primary structure analysis of an integral membrane glycoprotein of the nuclear pore. J. Cell Biol. 108: 2083-2092.
66. Wozniak, R. W., G. Blobel, and M. P. Rout. 1994. POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope. J. Cell Biol. 125:31-42.
67. Wu, J., M. J. Matunis, D. Kraemer, G. Blobel, and E. Coutavas. 1995. Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J. Biol Chem. 270:14209-14213
68. Yano, R., M. L. Oakes, M. M. Tabb, and M. Normura. 1994. Yeast Srp1p has homology to armadillo/plakoglobin/β-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure. Proc. Natl. Acad. Sci. USA 91:6880-6884.
69. Yokoyama, N., N. Hayashi, T. Seki, N. Pante, T. Ohba, K. Nishii, K. Kuma, T. Hajashida, T. Miyata, U. Abei, M. Fukui, and T. Nishimoto. 1995. A giant nucleopore protein that binds Ran/TC4. Nature (London) 376:184-188