Dependence of fluorodeoxyuridine-induced cytotoxicity and megabase DNA fragment formation on S phase progression in HT29 cells

Cancer Chemotherapy and Pharmacology

Volumn 37, Issue 5, 1996, Pages 486-490

  Tang, Hsin Yi Y ^a   Weber, Kris L ^a   Lawrence, Theodore S ^b   Merchant, Audrea K ^a   Maybaum, Jonathan ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Cell cycle; Double strand break; Fluorodeoxyuridine

Indexed keywords

ANTINEOPLASTIC ANTIMETABOLITE; DNA; FLOXURIDINE;

AGAR GEL ELECTROPHORESIS; ARTICLE; CELL CYCLE; CELL CYCLE S PHASE; CELL SEPARATION; CELL STRAIN HT29; CELL SURVIVAL; CHEMISTRY; COLON TUMOR; DNA BASE COMPOSITION; DNA DAMAGE; DRUG EFFECT; HUMAN; PATHOLOGY; COLON CARCINOMA; CONTROLLED STUDY; DRUG CYTOTOXICITY; HUMAN CELL; PRIORITY JOURNAL; TUMOR CELL LINE;

ANTIMETABOLITES, ANTINEOPLASTIC; BASE COMPOSITION; CELL CYCLE; CELL SEPARATION; CELL SURVIVAL; COLONIC NEOPLASMS; DNA DAMAGE; DNA, NEOPLASM; ELECTROPHORESIS, AGAR GEL; FLOXURIDINE; HT29 CELLS; HUMANS; S PHASE;

EID: 0029664593     PISSN: 03445704     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002800050416     Document Type: Article

References (16)

1. Bagwell CB, Hudson JL, Irvin GL (1979) Nonparametric flow cytometry analysis. J Histochem Cytochem 27: 293
2. Bhuyan BK, Scheidt LG, Fraser TJ (1972) Cell cycle phase specificity of antitumor agents. Cancer Res 32: 398
3. Bhuyan BK, Fraser TJ, Day KJ (1977) Cell proliferation kinetics and drug sensitivity of exponential and stationary populations of cultured L1210 cells. Cancer Res 37: 1057
4. Canman CE, Tang H-Y, Normolle DP, Lawrence TS, Maybaum J (1992) Variations in patterns of DNA damage induced in human colorectal tumor cells by 5-fluorodeoxyuridine - implications for mechanisms of resistance and cytotoxicity. Proc Natl Acad Sci USA 89: 10474
5. Canman CE, Lawrence TS, Shewach DS, Tang H-Y, Maybaum J (1993) Resistance to fluorodeoxyuridine-induced DNA damage and cytotoxicity correlates with an elevation of dUTPase activity and failure to accumulate dUTP. Cancer Res 53: 5219
6. Canman CE, Radany EH, Parsels LA, Davis MA, Lawrence TS, Maybaum J (1994) Induction of resistance to fluorodeoxyuridine cytotoxicity and DNA damage in human tumor cells by expression of Escherichia eoli deoxyuridinetriphosphatase. Cancer Res 54: 2296
7. Goulian M, Bleile BM, Dickey LM, Grafstrom RH, Ingraham HA, Neynaber SA, Peterson MS, Tseng BY (1986) Mechanism of thymineless death. Adv Exp Med Biol 195: 89
8. Hartwell LH, Kastan MB (1994) Cell cycle control and cancer. Science 266: 1821
9. Houghton JA, Harwood FG, Houghton PJ (1994) Cell cycle control processes determine cytostasis or cytotoxicity in thymineless death of colon cancer cells. Cancer Res 54: 4967
10. Kastan MB, Onyekwere O, Sidransky D, Vogelstein B, Craig RW (1991) Participation of p53 protein in the cellular response to DNA damage. Cancer Res 51: 6304
11. Lozzio CB (1969) Lethal effects of fluorodeoxyuridine on cultured mammalian cells at various stages of the cell cycle. J Cell Physiol 74: 57
12. Rodrigues NR, Rowan A, Smith ME, Kerr IB, Bodmer WF, Gannon JV, Lane DP (1990) p53 mutations in colorectal cancer. Proc Natl Acad Sci USA 87: 7555
13. Sedwick WD, Kutler M, Brown OE (1981) Antifolate-induced misincorporation of deoxyuridine monophosphate into DNA: inhibition of high molecular weight DNA synthesis in human lymphoblastoid cells. Proc Natl Acad Sci USA 78: 917
14. Tomita N, Jiang W, Hibshoosh H, Warburton D, Kahn SM, Weinstein IB (1992) Isolation and characterization of a highly malignant variant of the SW480 human colon cancer cell line. Cancer Res 52: 6840
15. Wilt SR, Burgess AC, Normolle DP, Trent JM, Lawrence TS (1994) Use of fluorescence in situ hybridization (FISH) to study chromosomal damage induced by radiation and bromodeoxyuridine in human colon cancer cells. Int J Radiat Oncol Biol Phys 30: 861
16. Yao KS, Clayton M, O'Dwyer PJ (1995) Apoptosis in human adenocarcinoma HT29 cells induced by exposure to hypoxia. J Natl Cancer Inst 87: 117