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Volumn 272, Issue 5260, 1996, Pages 414-417

Homocysteine antagonism of nitric oxide-related cytostasis in Salmonella typhimurium

Author keywords

[No Author keywords available]

Indexed keywords

SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0029664555     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5260.414     Document Type: Article
Times cited : (172)

References (35)
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    • note
    • After enrichment, the library was plated as individual colonies that were screened for GSNO susceptibility. Seven clones with highly increased susceptibility to GSNO were identified. The attL-junction fragments were cloned and sequenced as described (2) All seven clones were shown to have identical insertions between nucleotides 325 and 326 of the S. typhimurium metL gene. The sequenced region was shown to be 80% identical at the nucleotide level and >90% identical at the amino acid level to the corresponding region of E coli metL (22). We used bacteriophage P22 to transduce the metL.:Mud J insertion into a new wild-type background, 100% cotransduction of GSNO hypersusceptibility was confirmed.
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    • note
    • We are grateful to D Granger, E. Groisman, and J. Stamler for helpful suggestions and to K. Sanderson and A. Hessel for gifts of bactenal strains. Supported in part by grants from the U.S. Department of Agriculture (9401954), the National Institutes of Health (A132463), and the Thorkildsen Research Fellowship Program.


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