Binding of GSK3β to the APC-β-catenin complex and regulation of complex assembly

Science

Volumn 272, Issue 5264, 1996, Pages 1023-1026

  Rubinfeld, Bonnee ^a   Albert, Iris ^a   Porfiri, Emilio ^a   Fiol, Carol ^b   Munemitsu, Susan ^a   Polakis, Paul ^a  

^a ONYX PHARMACEUTICALS   (United States)

^b INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA CATENIN; CASEIN KINASE I; APC PROTEIN; CATNB PROTEIN, MOUSE; CTNNB1 PROTEIN, HUMAN; CYTOSKELETON PROTEIN; GLYCOGEN SYNTHASE KINASE; GLYCOGEN SYNTHASE KINASE 3; PROTEIN KINASE (CALCIUM,CALMODULIN); TRANSACTIVATOR PROTEIN;

ARTICLE; COLON POLYPOSIS; DROSOPHILA; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE COMPLEX; GENE SEQUENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; SEQUENCE HOMOLOGY; ANIMAL; CELL CULTURE; CELL LINE; HUMAN; METABOLISM; MOUSE; MUTATION; PHOSPHORYLATION; PROTEIN BINDING;

ADENOMATOUS POLYPOSIS COLI PROTEIN; ANIMALS; BETA CATENIN; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CELL LINE; CYTOSKELETAL PROTEINS; GLYCOGEN SYNTHASE KINASE 3; GLYCOGEN SYNTHASE KINASES; HUMANS; MICE; MUTATION; PHOSPHORYLATION; PROTEIN BINDING; TRANS-ACTIVATORS; TUMOR CELLS, CULTURED;

EID: 0029664368     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5264.1023     Document Type: Article

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33. 32P]ATP (10,000 cpm/pmol) was included in the reactions, and radioactive protein was collected by vacuum filtration over nitrocellulose filters. Phosphorylations in Fig. 4B were performed with unlabeled ATP and the phosphoproteins were repurified before being used for affinity precipitation of in vitro-translated β-catenin (wheat germ TNT system, Promega) (13).
34. We thank B. Souza and D. Lowe for help with production of recombinant proteins and F. McCormick for critical reading of the manuscript.