A new method for in vivo evaluation of antimicrobial agents by translocation of complex dense populations of cutaneous bacteria

Skin Pharmacology and Physiology

Volumn 9, Issue 1, 1996, Pages 60-68

  Leyden, James J ^a   McGinley, Kenneth J ^a   Foglia, Arlene N ^a   Wahrman, Julie E ^a   Gropper, Charles N ^a   Vowels, Benjamin R ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Cutaneous bacteria; In vitro testing; Topical antimicrobial

Indexed keywords

2 PROPANOL; BACTERICIDE; CHLORHEXIDINE; DETERGENT; FUSIDIC ACID; POVIDONE IODINE; PSEUDOMONIC ACID; TRICLOSAN; ANTIINFECTIVE AGENT;

ACTINOMYCETALES; ANTIMICROBIAL ACTIVITY; ARTICLE; AXILLA; BACTERIAL GROWTH; BACTERIAL SKIN DISEASE; BACTERIUM CULTURE; CONTROLLED STUDY; DRUG SCREENING; FEMALE; FOREARM; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE COCCI; HUMAN; HUMAN EXPERIMENT; INGUINAL REGION; MALE; METHODOLOGY; NORMAL HUMAN; PRIORITY JOURNAL; SKIN FLORA; TOPICAL DRUG ADMINISTRATION; ADULT; BACTERIUM; CLINICAL TRIAL; CULTURE MEDIUM; DRUG EFFECT; FUNGUS; MICROBIOLOGY; PERINEUM; PROTECTIVE CLOTHING; SKIN;

ADULT; ANTI-BACTERIAL AGENTS; ANTI-INFECTIVE AGENTS; AXILLA; BACTERIA; CULTURE MEDIA; FEMALE; FOREARM; FUNGI; HUMANS; MALE; OCCLUSIVE DRESSINGS; PERINEUM; SKIN;

EID: 0029662295     PISSN: 16605527     EISSN: 16605535     Source Type: Journal    
DOI: 10.1159/000211391     Document Type: Article

References (16)

1. Michaud RN, McGrath MB, Gross WA: Applications of a glovc-hand model for multiparameter measurements of skin-degcrming activity. J Clin Microbiol 1976:3:406-423.
2. Leyden JJ, McGitiley KJ, Kammer MS, Bakel J, Nishijima S, Grove MJ, Grove GL: Computerized image analysis of full-hand touch plates: A method for quantification of surface bacteria on hands and the effect of antimicrobial agents. J HospPract 1991;18:13-22.
3. Singh G: Pseudomonas infections of skin: An experimental study. Int J Dermatol 1974;13:90-92.
4. Leyden JJ, Stewart R, Kligman AM: Experimental infections in humans with group A streptococci. J Invest Dermatol 1980:75:196-201.
5. Leyden JJ, Stewart R, Kligman AM: Experimental inoculation of Pseudomonas aeruginosa and Pseudomonas cepacia on human skin. J Soc CosmetC'hem 1980:31:19-28.
6. Levden JJ, Stewart R, Kligman AM: Updated in vivo methods forevaluation of topical antimicrobial agents on human skin. J Invest Dermatol 1979;72:165-170.
7. Levdcn JJ, McGinlcv KJ, Holzle E, Labows JN: The microbiology of the human axilla and its relationship to axillary odor. J Invest Dermatol 1981;77:413-416.
8. Kates SG, Myung KB, McGinley KJ, Leyden JJ: Microbial ecology of interdigital infections of toe web spaces. J Am Acad Dermatol 1990; 22:578-582.
9. Balows H, Hausier WJ Jr, Herrman KL, Isenberg HD, Shadowy HJ: Manual of Clinical Microbiology. Washington. American Society for Microbiology. 1991.
10. Quisno RA, Doyle RL: A new occlusive patch test system with a plastic chamber. J Cosmct Chcm 1983:34: 13-19.
11. Kishihita M, Ushijima T, Ozaki Y, Ho Y: New medium for isolating propionibacteria and its application to assay of normal flora of human facial skin. Appl Environ Microbiol 1980:40:1100-1105.
12. Russell AD, Furr JR: Inactivation of human immunodeficiency virus by chlorhexidine: The possible role of neutralizes (letter). J Hosp Infect 1991;18:249-251.
13. Williamson P, Kligman AM: A new method for the quantitative investigation of cutaneous bacteria. J Invest Dermatol 1965,45:465-473.
14. Aly R, Shirley C, Cunico B, Maibach HI: Effect of prolonged occlusion on the microbial flora, pH, carbon dioxide and transepidermal water loss on human skin. J Invest Dermatol 1978;71:378-381.
15. Godtfredsen W, Roholt K, Tybring L: Fucidin: A new orally active antibiotic. Lancet 1962.
16. Fuller AT, Mellows G, Woolford M, Banks GT, Barrow KD, Chain EB: Pscudomonic acid: An antibiotic produced by Pseudomonas Jluores-cens. Nature 1971:234:416-417.